##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961970
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.677669781801928	31.0	31.0	34.0	30.0	34.0
2	31.87081717725085	31.0	31.0	34.0	30.0	34.0
3	31.95844153144069	31.0	31.0	34.0	30.0	34.0
4	35.65106500202709	37.0	35.0	37.0	33.0	37.0
5	35.552824932170445	37.0	35.0	37.0	33.0	37.0
6	35.5714866367974	37.0	35.0	37.0	33.0	37.0
7	35.44567606058401	37.0	35.0	37.0	33.0	37.0
8	35.45978149006726	37.0	35.0	37.0	33.0	37.0
9	37.07453766749483	39.0	37.0	39.0	33.0	39.0
10	36.933083152281256	39.0	37.0	39.0	33.0	39.0
11	36.98502863914675	39.0	37.0	39.0	33.0	39.0
12	36.876216514028506	39.0	37.0	39.0	33.0	39.0
13	36.916447498362736	39.0	37.0	39.0	33.0	39.0
14	37.95224591203468	40.0	37.0	41.0	33.0	41.0
15	37.905636350405935	40.0	37.0	41.0	33.0	41.0
16	37.81573749701134	40.0	37.0	41.0	32.0	41.0
17	37.83530359574623	40.0	37.0	41.0	32.0	41.0
18	37.801174672806845	40.0	37.0	41.0	32.0	41.0
19	37.81550776011726	40.0	37.0	41.0	32.0	41.0
20	37.70106760086073	40.0	37.0	41.0	32.0	41.0
21	37.59345613688576	39.0	37.0	41.0	32.0	41.0
22	37.442327723317774	39.0	36.0	41.0	32.0	41.0
23	37.31173113506658	39.0	36.0	41.0	31.0	41.0
24	37.18062517542127	39.0	36.0	41.0	31.0	41.0
25	36.98478850691809	39.0	36.0	41.0	31.0	41.0
26	37.142927534122684	39.0	36.0	41.0	31.0	41.0
27	37.077711363140224	39.0	36.0	41.0	31.0	41.0
28	36.96431905360874	39.0	36.0	41.0	31.0	41.0
29	36.821629572647794	39.0	35.0	41.0	30.0	41.0
30	36.70261858477915	39.0	35.0	40.0	30.0	41.0
31	36.53780991091198	39.0	35.0	40.0	30.0	41.0
32	36.441018950694925	39.0	35.0	40.0	30.0	41.0
33	36.25100263001965	38.0	35.0	40.0	30.0	41.0
34	36.1138382693847	38.0	35.0	40.0	29.0	41.0
35	35.93659157769993	38.0	35.0	40.0	29.0	41.0
36	35.80342630227554	38.0	35.0	40.0	28.0	41.0
37	35.647582564944855	38.0	35.0	40.0	27.0	41.0
38	35.57469359751344	38.0	35.0	40.0	27.0	41.0
39	35.45707142634386	38.0	34.0	40.0	26.0	41.0
40	35.28676569955404	38.0	34.0	40.0	25.0	41.0
41	35.17860432238012	38.0	34.0	40.0	25.0	41.0
42	34.97097310726946	38.0	34.0	40.0	24.0	41.0
43	34.62636880568001	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	3.0
15	7.0
16	16.0
17	16.0
18	73.0
19	139.0
20	331.0
21	672.0
22	1162.0
23	2049.0
24	3404.0
25	4989.0
26	7603.0
27	10652.0
28	14951.0
29	19692.0
30	24770.0
31	31391.0
32	38221.0
33	47274.0
34	58649.0
35	72636.0
36	94425.0
37	133502.0
38	184326.0
39	211013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.058692059003924	20.60989427944738	13.312785221992371	24.018628439556327
2	19.09664542553302	22.472946141771573	34.932586255288626	23.49782217740678
3	19.851866482322734	24.07195650592014	32.065345073131176	24.010831938625945
4	13.831512417227149	15.852989178456708	36.328783641901516	33.98671476241463
5	13.316111729055999	37.82508810046051	34.94246182313378	13.916338347349711
6	33.05539673794401	36.90063099680863	13.846169838976266	16.19780242627109
7	26.8937700759899	32.17553561961392	21.54453881098163	19.386155493414556
8	27.152613906878592	34.20033888790711	18.967223510088672	19.679823695125627
9	24.91179558614094	14.200234934561367	21.34058234664283	39.54738713265486
10	16.708213353846794	26.75135399232824	33.62558083931931	22.91485181450565
11	34.01509402580122	22.07438901421042	23.173487738702867	20.73702922128549
12	21.152322837510525	26.388348909009636	30.769878478538832	21.689449774941004
13	31.258771063546682	20.612285206399367	25.93635976173893	22.19258396831502
14	20.96822146220776	22.22782415252035	26.98181855983035	29.822135825441542
15	23.457072465877317	29.035416904893086	24.62581993201451	22.88169069721509
16	22.780440138465856	27.787456989303198	25.92263792010146	23.509464952129484
17	22.45236337931536	26.2843955632712	26.535027079846564	24.728213977566867
18	23.350312379803945	25.447363223385345	28.617940268407537	22.584384128403173
19	23.494599623688888	24.734347224965436	29.331995800284833	22.439057351060843
20	24.247429753526617	24.495774296495732	29.107664480181295	22.149131469796355
21	23.339813091884363	25.021362412549248	29.525452976704052	22.113371518862333
22	22.403401353472564	25.54352006819339	29.02658087050532	23.026497707828728
23	22.902793226399993	25.752986059856337	29.914134536420057	21.430086177323616
24	23.01631027994636	25.765148601307736	27.745251930933396	23.47328918781251
25	23.16132519725147	25.65828456188862	28.62916722974729	22.55122301111261
26	22.823476823601567	26.364959406218492	28.66565485410148	22.145908916078465
27	22.08509620882148	26.44864184953793	28.33404368119588	23.132218260444713
28	22.403817166855518	26.09249768703806	28.584883104462715	22.91880204164371
29	22.748838321361372	26.103724648377806	28.059191035063463	23.088245995197358
30	22.4367703774546	26.26724326122436	29.426697298252545	21.869289063068496
31	22.94998804536524	25.90548561805462	29.443433786916433	21.701092549663713
32	22.49508820441386	25.78791438402445	28.45161491522605	23.265382496335647
33	22.331465638221566	25.80912086655509	29.08562637088475	22.773787124338597
34	21.834568645591858	26.275143715500484	29.439275653086895	22.451011985820763
35	21.520213728078836	27.937981433932453	28.490805326569436	22.050999511419274
36	23.198956308408786	25.976069939811016	28.512635529174506	22.312338222605693
37	22.364522802166388	26.37888915454744	28.45462956225246	22.80195848103371
38	22.19653419545308	25.246213499381476	29.65560256556857	22.90164973959687
39	21.71564601806709	25.150160607919165	30.37402413796688	22.760169236046863
40	21.02799463600736	25.79519111822614	29.936796365791036	23.240017879975465
41	21.932076883894506	24.70960632867969	30.129110055407132	23.22920673201867
42	20.72216389284489	26.44219674210214	29.74957639011612	23.08606297493685
43	21.092653617056666	26.151647140763224	29.10745657348982	23.64824266869029
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	95.5
2	147.0
3	423.5
4	700.0
5	700.0
6	1049.5
7	1399.0
8	1539.5
9	1680.0
10	2423.5
11	3167.0
12	3167.0
13	5877.5
14	8588.0
15	13568.0
16	18548.0
17	17229.5
18	15911.0
19	15911.0
20	16915.5
21	17920.0
22	14794.5
23	11669.0
24	13358.0
25	15047.0
26	15047.0
27	16674.5
28	18302.0
29	20108.5
30	21915.0
31	24273.5
32	26632.0
33	26632.0
34	30106.5
35	33581.0
36	37073.0
37	40565.0
38	43914.5
39	47264.0
40	47264.0
41	49395.5
42	51527.0
43	55359.0
44	59191.0
45	61381.0
46	63571.0
47	63571.0
48	79094.0
49	94617.0
50	91307.0
51	87997.0
52	82242.0
53	76487.0
54	76487.0
55	69478.0
56	62469.0
57	54641.5
58	46814.0
59	42037.0
60	37260.0
61	37260.0
62	33077.5
63	28895.0
64	25475.0
65	22055.0
66	18940.5
67	15826.0
68	15826.0
69	13505.0
70	11184.0
71	9699.5
72	8215.0
73	6801.0
74	5387.0
75	5387.0
76	4373.0
77	3359.0
78	2608.5
79	1858.0
80	1443.5
81	1029.0
82	1029.0
83	781.5
84	534.0
85	409.0
86	284.0
87	231.5
88	179.0
89	179.0
90	134.5
91	90.0
92	66.0
93	42.0
94	27.0
95	12.0
96	12.0
97	9.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	961970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.84965856417141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.76638736786553	47.62090521017277
2	9.104687163483767	10.351967131552929
3	2.6853886484225162	4.579902833247654
4	1.1978462119967104	2.723885926575963
5	0.6987359178188065	1.9861449177261044
6	0.4798550522831425	1.6367757529553564
7	0.3276596110158493	1.3039135908064174
8	0.24885290218388023	1.131776201748539
9	0.17936089201077088	0.917694492351979
>10	1.146381352203065	12.142132250126703
>50	0.10500503647882396	4.098425804179015
>100	0.05451666797535379	5.962463694434729
>500	0.0031204826361659163	1.3124348999674562
>1k	0.0016520202191466617	1.7853580904463076
>5k	5.506734063822205E-4	2.446219203708127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9187	0.9550193872989802	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7655	0.7957628616277015	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6600	0.6860920818736551	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3302	0.3432539476283044	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2264	0.23535037475181136	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2093	0.21757435263053942	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2092	0.217470399284801	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1880	0.19543228998825327	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1678	0.1744337141490899	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1531	0.15915257232554028	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1203	0.12505587492333442	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1066	0.11081426655716914	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	985	0.10239404555235611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.1186003721529775E-4	0.0
10	0.0	0.0	0.0	3.1186003721529775E-4	0.0
11	0.0	0.0	0.0	4.1581338295373037E-4	0.0
12	0.0	0.0	0.0	7.276734201690281E-4	0.0
13	1.0395334573843259E-4	0.0	0.0	8.316267659074607E-4	0.0
14	1.0395334573843259E-4	0.0	0.0	9.355801116458934E-4	0.0
15	1.0395334573843259E-4	0.0	0.0	0.0013513934945996236	0.0
16	2.0790669147686519E-4	0.0	0.0	0.0019751135690302193	0.0
17	2.0790669147686519E-4	0.0	0.0	0.002494880297722382	0.0
18	3.1186003721529775E-4	0.0	0.0	0.0027027869891992472	0.0
19	3.1186003721529775E-4	0.0	0.0	0.0029106936806761125	0.0
20	3.1186003721529775E-4	0.0	0.0	0.0047818539039679	0.0
21	3.1186003721529775E-4	0.0	0.0	0.009979521190889528	0.0
22	4.1581338295373037E-4	0.0	0.0	0.017360208738318242	0.0
23	4.1581338295373037E-4	0.0	0.0	0.021102529184901814	0.0
24	4.1581338295373037E-4	0.0	0.0	0.027547636620684636	0.0
25	4.1581338295373037E-4	0.0	0.0	0.03305716394482156	0.0
26	4.1581338295373037E-4	0.0	0.0	0.03991808476355811	0.0
27	4.1581338295373037E-4	0.0	0.0	0.08368244331943823	0.0
28	4.1581338295373037E-4	0.0	0.0	0.1789037080158425	0.0
29	4.1581338295373037E-4	0.0	0.0	0.3012567959499777	0.0
30	4.1581338295373037E-4	0.0	0.0	0.4673742424399929	0.0
31	5.19766728692163E-4	0.0	0.0	0.7054274041810036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCATCG	65	2.6820908E-6	22.76923	19
GGTATCA	2480	0.0	22.080647	1
TATAGAC	80	6.957689E-7	20.8125	3
AGCCTAC	50	0.007034069	18.5	6
TAATACT	210	0.0	17.619047	4
GTATTGG	505	0.0	17.58416	1
TACACTA	85	2.7223678E-5	17.411764	5
TAAGCCG	75	2.0668253E-4	17.266666	10
ATATAAC	120	1.04064384E-7	16.958332	3
ATTAGAC	110	7.8043377E-7	16.818182	3
ACCGTTA	165	5.4569682E-11	16.818182	8
CTTAATA	80	3.3818482E-4	16.1875	2
GCGATTT	70	0.0025920807	15.857143	30
TCTATGC	70	0.0025920807	15.857143	3
CGCCGTT	190	2.7284841E-11	15.578948	25
CGTTTAT	95	7.0577626E-5	15.578948	1
GACCGTT	170	1.4842954E-9	15.235294	7
ATACTGC	110	1.4513002E-5	15.136364	6
ATAACTG	220	1.8189894E-12	15.136364	5
AATACTG	220	1.8189894E-12	15.136364	5
>>END_MODULE