FastQCFastQC Report
Wed 25 May 2016
SRR2088642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647076
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175991.068499571361613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149770.9093083743555245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124010.7529100053670869No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61630.37417824071263256No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37430.22725120152318415No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35990.21850843555488636No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA35740.21699059424094574No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG34380.20873353749310902No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA30600.18578377682632738No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG27290.16568755782975406No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19430.11796662691946212No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.10946671556139485No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA16670.10120965881355809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.022.9170821
GTATTAG2150.019.7906971
GTATTGG6750.017.2666681
ATAGGAC752.0679514E-417.2666663
ACGTTTA700.002593132715.85714326
ACCGTTA2450.015.8571428
GTTATAC1059.347934E-615.8571423
CCGTTTA2400.015.41666627
GTATCAA75000.015.3180012
CTAATAC3900.015.1794873
CGTTTAC2450.015.1020428
TCTAATA3850.014.8961052
CGTTATT1252.9604435E-614.80000110
TGTTACG1252.9604435E-614.80000116
TAATACT4250.014.84
CTGAGCG1508.107054E-814.89
TCGGGTA1904.5656634E-1014.60526425
CTCTAAT3300.014.5757571
GACCGTT2950.014.4237297
GGACCGT3500.014.2714296
Produced by FastQC (version 0.11.5)