##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647076
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543574795577133	31.0	31.0	34.0	30.0	34.0
2	31.730047672359987	31.0	31.0	34.0	30.0	34.0
3	31.806475232472575	31.0	31.0	34.0	30.0	34.0
4	35.52717543088479	37.0	35.0	37.0	33.0	37.0
5	35.41938805495314	37.0	35.0	37.0	33.0	37.0
6	35.44889064014047	37.0	35.0	37.0	33.0	37.0
7	35.31635820083591	37.0	35.0	37.0	32.0	37.0
8	35.334880721958186	37.0	35.0	37.0	32.0	37.0
9	36.921571317899115	39.0	37.0	39.0	33.0	39.0
10	36.7699590061418	39.0	37.0	39.0	32.0	39.0
11	36.824386974250125	39.0	37.0	39.0	32.0	39.0
12	36.70803047339649	39.0	35.0	39.0	32.0	39.0
13	36.754147956742734	39.0	35.0	39.0	32.0	39.0
14	37.74192872702899	40.0	37.0	41.0	32.0	41.0
15	37.70150193433697	39.0	37.0	41.0	32.0	41.0
16	37.598831505042874	39.0	36.0	41.0	32.0	41.0
17	37.628661943954015	39.0	36.0	41.0	32.0	41.0
18	37.60608314370436	39.0	36.0	41.0	32.0	41.0
19	37.62966371922121	39.0	37.0	41.0	32.0	41.0
20	37.50966197066802	39.0	36.0	41.0	32.0	41.0
21	37.406262977543236	39.0	36.0	41.0	32.0	41.0
22	37.2528438274858	39.0	36.0	41.0	31.0	41.0
23	37.112871537196824	39.0	36.0	40.0	31.0	41.0
24	36.96848779291302	39.0	36.0	40.0	31.0	41.0
25	36.75928797456827	39.0	35.0	40.0	30.0	41.0
26	36.91466271137458	39.0	36.0	40.0	31.0	41.0
27	36.84167579395243	39.0	35.0	40.0	30.0	41.0
28	36.715864355986	39.0	35.0	40.0	30.0	41.0
29	36.56636730788379	39.0	35.0	40.0	30.0	41.0
30	36.445429354808155	39.0	35.0	40.0	30.0	41.0
31	36.26188773317078	38.0	35.0	40.0	30.0	41.0
32	36.14870169925371	38.0	35.0	40.0	30.0	41.0
33	35.94772250946526	38.0	35.0	40.0	29.0	41.0
34	35.80575152573409	38.0	35.0	40.0	28.0	41.0
35	35.616907780818856	38.0	34.0	40.0	27.0	41.0
36	35.479112074974076	38.0	34.0	40.0	26.0	41.0
37	35.31436497162244	38.0	34.0	40.0	26.0	41.0
38	35.24249093545167	38.0	34.0	40.0	25.0	41.0
39	35.11114726946419	38.0	34.0	40.0	25.0	41.0
40	34.92927284472605	38.0	34.0	40.0	24.0	41.0
41	34.81138818123754	38.0	33.0	40.0	24.0	41.0
42	34.59568532356734	38.0	33.0	40.0	23.0	41.0
43	34.2328514288351	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	3.0
14	6.0
15	15.0
16	22.0
17	41.0
18	146.0
19	290.0
20	719.0
21	1371.0
22	2508.0
23	4299.0
24	6916.0
25	10592.0
26	15070.0
27	20963.0
28	28666.0
29	36951.0
30	46591.0
31	57374.0
32	69882.0
33	85548.0
34	104129.0
35	128334.0
36	166049.0
37	230635.0
38	313955.0
39	315996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32851185980489	21.382619866964244	13.940826045671237	23.348042227559628
2	19.143500360639095	22.962328392861046	34.951696218025155	22.942475028474703
3	19.96695963027814	24.717863656564724	32.017769671830564	23.29740704132657
4	14.185198497215673	16.37283282617196	36.01655297023331	33.42541570637906
5	13.110323992335509	38.22780491003451	34.70022026913148	13.961650828498504
6	32.700919690408945	37.197676367089315	13.72183190089589	16.379572041605854
7	26.28075450070306	32.58884228778757	21.706527203359165	19.423876008150202
8	26.736289035842915	34.785462237322385	19.067365440331837	19.41088328650287
9	24.666742761111205	14.494352416039089	21.439751414020968	39.39915340882874
10	16.67749393470611	27.057828539788087	33.444661934239825	22.820015591265978
11	33.58958542289487	22.311478037443326	23.201540183938082	20.897396355723718
12	20.955985030441827	26.840048668063893	30.820678584351906	21.38328771714238
13	31.273056009558758	20.80353304886963	26.167644965988213	21.755765975583397
14	20.739419431768784	22.50466887988168	27.313858012623584	29.442053675725955
15	23.22151497562954	29.16811367538596	25.073281378637052	22.537089970347452
16	22.250885812190816	27.97454397975564	26.19120186318057	23.583368344872973
17	22.03899516476471	26.5255519478154	26.828877355993285	24.6065755314266
18	23.150904997705023	25.415342097146702	29.031690098088976	22.4020628070593
19	23.19012601725725	24.80966269923185	29.79152145984763	22.208689823663267
20	24.001624697342443	24.522729977244524	29.6375516369615	21.838093688451536
21	23.024924168647956	25.26173655617591	29.91993083500701	21.793408440169124
22	21.967049486483926	25.607257952881348	29.6363980775629	22.78929448307182
23	22.681831318044825	25.87543015622837	30.427071974820834	21.01566655090597
24	22.74351638904337	25.883080076450632	28.274955132610756	23.09844840189524
25	22.703081096439995	25.809980838771253	29.176127877523562	22.31081018726519
26	22.485969074893934	26.465688286393586	29.23016302829985	21.81817961041263
27	21.668277602247862	26.561980139349977	28.957194446400774	22.812547812001387
28	21.994309916482297	26.161330746122218	29.208852536252124	22.63550680114336
29	22.278449810451978	26.176509159261624	28.6953971765723	22.849643853714095
30	22.128729943244878	26.293807936002956	30.019076229633608	21.558385891118565
31	22.570786047516933	25.8824122262725	29.97129458507076	21.575507141139813
32	22.263332110965127	25.778470453093842	29.063929047597075	22.89426838834395
33	22.002263404967348	25.775616911423636	29.618973259278867	22.603146424330145
34	21.49827937508652	26.16673426119985	30.044333109097575	22.290653254616057
35	21.227860766594866	27.73581789789906	29.15099242536471	21.885328910141368
36	22.815583494629273	25.686367842163932	29.18001355128725	22.318035111919546
37	21.94853182245385	26.207412408413454	29.160463755163697	22.683592013968997
38	21.800026228297906	24.938800638221917	30.479225002367833	22.781948131112348
39	21.233203568019935	24.93278998661871	31.22709577457264	22.606910670788718
40	20.463354453589268	25.57095118865189	30.771196957517443	23.1944974002414
41	21.38887337317768	24.71926007057355	30.804346611813905	23.087519944434863
42	20.2633029684119	26.4064924751499	30.278930662580233	23.051273893857964
43	20.598867326097885	26.197576796699117	29.6339695314606	23.569586345742394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	173.5
2	259.0
3	715.0
4	1171.0
5	1171.0
6	1863.0
7	2555.0
8	2869.0
9	3183.0
10	4791.0
11	6399.0
12	6399.0
13	11516.5
14	16634.0
15	26163.5
16	35693.0
17	32980.0
18	30267.0
19	30267.0
20	32120.0
21	33973.0
22	27496.5
23	21020.0
24	23932.5
25	26845.0
26	26845.0
27	29623.5
28	32402.0
29	35834.5
30	39267.0
31	43342.0
32	47417.0
33	47417.0
34	52912.0
35	58407.0
36	63851.0
37	69295.0
38	74592.0
39	79889.0
40	79889.0
41	83265.5
42	86642.0
43	92989.5
44	99337.0
45	105759.0
46	112181.0
47	112181.0
48	139019.0
49	165857.0
50	157845.0
51	149833.0
52	139573.5
53	129314.0
54	129314.0
55	116804.5
56	104295.0
57	90525.5
58	76756.0
59	68739.0
60	60722.0
61	60722.0
62	53872.0
63	47022.0
64	41032.5
65	35043.0
66	30254.0
67	25465.0
68	25465.0
69	21713.5
70	17962.0
71	15314.0
72	12666.0
73	10389.5
74	8113.0
75	8113.0
76	6480.0
77	4847.0
78	3839.5
79	2832.0
80	2242.5
81	1653.0
82	1653.0
83	1248.5
84	844.0
85	645.5
86	447.0
87	337.0
88	227.0
89	227.0
90	187.5
91	148.0
92	109.0
93	70.0
94	45.5
95	21.0
96	21.0
97	15.5
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1647076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.43946923501559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05964811019449	45.21723157966153
2	9.610542892191198	10.46385708222481
3	2.810562471361153	4.5901658757826445
4	1.2474107581811875	2.716335183737289
5	0.7120996946400032	1.9383164709309224
6	0.46055518657296357	1.504342794627943
7	0.32505788576224626	1.2387185140087111
8	0.2303308905747781	1.00312731450555
9	0.18553291852400636	0.9090282249063268
>10	1.1809409369955062	12.219816051933568
>50	0.10321264559533118	3.915605820403547
>100	0.06638179721172398	6.697138474421154
>500	0.004813390208791926	1.8147683246112365
>1k	0.002462664757986567	2.6551194057525715
>5k	1.119393071812076E-4	0.3755680720140715
>10k+	3.358179215436228E-4	2.740860810478159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17599	1.068499571361613	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14977	0.9093083743555245	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12401	0.7529100053670869	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6163	0.37417824071263256	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3743	0.22725120152318415	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3599	0.21850843555488636	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3574	0.21699059424094574	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3438	0.20873353749310902	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3060	0.18578377682632738	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2729	0.16568755782975406	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1943	0.11796662691946212	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1803	0.10946671556139485	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1667	0.10120965881355809	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.071365255762333E-5	0.0	0.0
2	0.0	0.0	6.071365255762333E-5	0.0	0.0
3	0.0	0.0	6.071365255762333E-5	0.0	0.0
4	0.0	0.0	6.071365255762333E-5	0.0	0.0
5	6.071365255762333E-5	0.0	6.071365255762333E-5	0.0	0.0
6	6.071365255762333E-5	0.0	1.2142730511524665E-4	0.0	0.0
7	6.071365255762333E-5	0.0	1.2142730511524665E-4	0.0	0.0
8	1.2142730511524665E-4	0.0	1.2142730511524665E-4	0.0	0.0
9	1.2142730511524665E-4	0.0	1.2142730511524665E-4	3.035682627881166E-4	0.0
10	1.2142730511524665E-4	0.0	1.2142730511524665E-4	6.678501781338566E-4	0.0
11	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.0010321320934795965	0.0
12	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.0014571276613829598	0.0
13	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.0015785549664982066	0.0
14	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.00218569149207444	0.0
15	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.002428546102304933	0.0
16	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.003157109932996413	0.0
17	1.8214095767286998E-4	0.0	1.2142730511524665E-4	0.004432096636706503	0.0
18	2.428546102304933E-4	0.0	1.2142730511524665E-4	0.005282087772513229	0.0
19	2.428546102304933E-4	0.0	1.2142730511524665E-4	0.006921356391569059	0.0
20	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.011839162248736549	0.0
21	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.020824782827264802	0.0
22	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.0361853369243435	0.0
23	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.04504953019775651	0.0
24	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.05962080681158611	0.0
25	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.06787786355942288	0.0
26	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.07801704353654598	0.0
27	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.12998793012587154	0.0
28	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.2591258691159364	0.0
29	3.6428191534573995E-4	0.0	1.2142730511524665E-4	0.42305273102151936	0.0
30	4.857092204609866E-4	0.0	1.2142730511524665E-4	0.6638430770650534	0.0
31	5.4642287301861E-4	0.0	1.2142730511524665E-4	1.024967882477797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5005	0.0	22.917082	1
GTATTAG	215	0.0	19.790697	1
GTATTGG	675	0.0	17.266668	1
ATAGGAC	75	2.0679514E-4	17.266666	3
ACGTTTA	70	0.0025931327	15.857143	26
ACCGTTA	245	0.0	15.857142	8
GTTATAC	105	9.347934E-6	15.857142	3
CCGTTTA	240	0.0	15.416666	27
GTATCAA	7500	0.0	15.318001	2
CTAATAC	390	0.0	15.179487	3
CGTTTAC	245	0.0	15.10204	28
TCTAATA	385	0.0	14.896105	2
CGTTATT	125	2.9604435E-6	14.800001	10
TGTTACG	125	2.9604435E-6	14.800001	16
TAATACT	425	0.0	14.8	4
CTGAGCG	150	8.107054E-8	14.8	9
TCGGGTA	190	4.5656634E-10	14.605264	25
CTCTAAT	330	0.0	14.575757	1
GACCGTT	295	0.0	14.423729	7
GGACCGT	350	0.0	14.271429	6
>>END_MODULE