FastQCFastQC Report
Wed 25 May 2016
SRR2088641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427323
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT473731.3822157993279303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385261.1240843071983586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323810.944789854939263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171530.5004780699105396No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG90450.2639085957174156No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG82890.24185056383655695No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA82280.2400707490948475No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA79480.23190110765749244No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA76210.22236013355029566No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69810.20368666740776986No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49870.14550714945746288No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA46880.1367831394940016No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46410.13541180682415985No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43980.12832172514816959No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42500.12400348610271048No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.12347829486745193No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40520.11822638251486656No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG37860.11046522314937927No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC37030.10804350800902045No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35550.10372526896356137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141850.020.5932331
ACGTTTA1700.018.526
GTATTGG16450.018.3313061
TATACCG1900.017.5263165
ACCGTTA6850.016.4744538
GTATTAG7100.016.154931
GAACCGT1504.6802597E-916.0333336
ATTGGAC20750.015.2457833
TACCCCG4400.015.1363645
TTGGACC25600.015.031254
ATTAGAG6000.014.83
TGGACCC25650.014.6413265
TAATACT8350.014.6227544
TGTTACG4950.014.57575816
TATTGGA22550.014.2749432
AATACTG8700.014.0344825
GACCGTT7950.013.7295597
GGACCCT27250.013.7137616
TATACAC19000.013.5342137
TCTAGAC2601.8189894E-1213.5192313
Produced by FastQC (version 0.11.5)