##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088641_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3427323
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.627602650815227	31.0	31.0	34.0	30.0	34.0
2	31.800758492852875	31.0	31.0	34.0	30.0	34.0
3	31.87653483491343	31.0	31.0	34.0	30.0	34.0
4	35.57180224916064	37.0	35.0	37.0	33.0	37.0
5	35.48741539679803	37.0	35.0	37.0	33.0	37.0
6	35.52452657657303	37.0	35.0	37.0	33.0	37.0
7	35.38561057711806	37.0	35.0	37.0	33.0	37.0
8	35.4174327893811	37.0	35.0	37.0	33.0	37.0
9	37.001634803606194	39.0	37.0	39.0	33.0	39.0
10	36.8755495178015	39.0	37.0	39.0	33.0	39.0
11	36.91893352333585	39.0	37.0	39.0	33.0	39.0
12	36.82033091132642	39.0	37.0	39.0	32.0	39.0
13	36.86827445210154	39.0	37.0	39.0	33.0	39.0
14	37.85638412253528	40.0	37.0	41.0	32.0	41.0
15	37.82067257740225	40.0	37.0	41.0	32.0	41.0
16	37.71535597899585	40.0	37.0	41.0	32.0	41.0
17	37.77535003266398	40.0	37.0	41.0	32.0	41.0
18	37.7630217519621	40.0	37.0	41.0	32.0	41.0
19	37.793740770858186	40.0	37.0	41.0	32.0	41.0
20	37.68211458330598	40.0	37.0	41.0	32.0	41.0
21	37.57646886505882	39.0	37.0	41.0	32.0	41.0
22	37.427183256436585	39.0	36.0	41.0	32.0	41.0
23	37.297531922144486	39.0	36.0	41.0	31.0	41.0
24	37.15054431694941	39.0	36.0	41.0	31.0	41.0
25	36.946536991115224	39.0	36.0	41.0	31.0	41.0
26	37.079543713854804	39.0	36.0	41.0	31.0	41.0
27	36.99720481553679	39.0	36.0	41.0	31.0	41.0
28	36.86527006646295	39.0	35.0	41.0	30.0	41.0
29	36.70815940020827	39.0	35.0	41.0	30.0	41.0
30	36.55931232626747	39.0	35.0	40.0	30.0	41.0
31	36.34866191485308	38.0	35.0	40.0	30.0	41.0
32	36.22181189225527	38.0	35.0	40.0	30.0	41.0
33	36.003169820877694	38.0	35.0	40.0	29.0	41.0
34	35.85139072097961	38.0	35.0	40.0	29.0	41.0
35	35.64930005138121	38.0	35.0	40.0	27.0	41.0
36	35.48286519829033	38.0	34.0	40.0	27.0	41.0
37	35.291676039871355	38.0	34.0	40.0	26.0	41.0
38	35.16787212643804	38.0	34.0	40.0	25.0	41.0
39	35.010729656936334	38.0	34.0	40.0	24.0	41.0
40	34.799278620661084	38.0	33.0	40.0	24.0	41.0
41	34.643149770243426	38.0	33.0	40.0	23.0	41.0
42	34.38217290871039	38.0	33.0	40.0	21.0	41.0
43	34.003816681415785	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	6.0
14	12.0
15	19.0
16	47.0
17	114.0
18	265.0
19	591.0
20	1263.0
21	2694.0
22	4937.0
23	8385.0
24	13821.0
25	21118.0
26	30551.0
27	43167.0
28	58204.0
29	75416.0
30	95091.0
31	115620.0
32	141381.0
33	174538.0
34	214703.0
35	263389.0
36	340281.0
37	483446.0
38	633108.0
39	705148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08030582469175	21.11041182870713	14.026165610886398	22.783116735714724
2	19.08938842355973	23.421311618426394	34.80042587173721	22.688874086276662
3	20.16935666699637	24.6594499555484	32.583710376874315	22.587483000580917
4	13.779675857805056	16.19179166947498	36.13187318499015	33.89665928772981
5	12.963937160285155	38.5348273273339	34.49307228994758	14.008163222433367
6	32.37325457798988	36.89961523906559	13.590490303948592	17.13663987899594
7	25.850817095441542	33.03377008820003	21.537012998191297	19.578399818167124
8	27.284151508334638	34.119457080642825	18.919576590826136	19.6768148201964
9	24.434288802076722	14.446260244511533	22.09561223147045	39.023838721941296
10	16.898319767351953	27.221507864884636	33.05113057625441	22.829041791509
11	32.99426403639225	22.458315133998166	23.5504503077183	20.996970521891285
12	21.110032523926108	27.226730599946375	30.854226461877097	20.809010414250423
13	31.596059081679783	20.7275765954945	26.459309496070254	21.217054826755458
14	20.435570268690753	23.094584315513885	27.993684867168923	28.476160548626435
15	23.024792235806196	29.26730278996173	25.73699648384468	21.970908490387394
16	21.67683057593346	28.167698229784587	26.88856579902157	23.26690539526038
17	21.701543741281462	26.540743314826177	27.300023954555787	24.457688989336575
18	22.756419514589084	25.234038344212085	29.851694748350244	22.157847392848588
19	22.725900068362392	24.767814413756742	30.91990454357526	21.5863809743056
20	23.724784620533285	24.30059845541258	30.66346533431486	21.31115158973928
21	22.498346377041205	25.28413575259758	31.185388712998453	21.032129157362757
22	21.248186996089952	25.743298778667782	30.7477877048647	22.260726520377567
23	22.020188934629157	25.907187621359295	31.70033871916945	20.3722847248421
24	22.175616362974836	26.01870322697919	29.213908347710444	22.59177206233553
25	22.09742122350301	26.100603882388672	30.269834503488585	21.532140390619734
26	21.76958518353829	26.800712976279158	30.345958055310224	21.083743784872333
27	20.8225778544946	26.86507807988917	29.956032740421605	22.356311325194618
28	21.121820149428576	26.606859055887057	30.179384901860722	22.091935892823642
29	21.51688066750639	26.5866975479113	29.596276744269506	22.300145040312806
30	21.40443138857937	26.63548197820865	31.299034260850235	20.661052372361752
31	21.957953773251017	26.368217994043746	31.00314735436374	20.670680878341493
32	21.614828832882107	26.173926414288935	29.91419250534601	22.29705224748295
33	21.211131836713378	26.252209085633304	30.548886113155955	21.987772964497367
34	20.582857232889925	26.74492599617836	31.072034938055154	21.600181832876565
35	20.302959481787973	28.56620166818243	29.971117399789865	21.159721450239736
36	22.279516695683483	26.348202372522227	29.92673874041052	21.445542191383772
37	21.27255003394778	26.883868255195086	29.925950953557628	21.917630757299502
38	21.094393496031742	25.42555808133637	31.297487864435304	22.182560558196588
39	20.5470566970198	25.418001163006814	31.95067987464269	22.084262265330697
40	19.574956897847095	26.175939647357428	31.597693009967255	22.651410444828223
41	20.831768701111624	25.04499867680986	31.65170017532634	22.471532446752175
42	19.639555419783896	26.886114906590365	31.052281912151265	22.42204776147448
43	19.876299957722104	26.71035090652384	30.330844218651116	23.082504917102938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	451.0
2	691.0
3	2038.0
4	3385.0
5	3385.0
6	5227.0
7	7069.0
8	7881.0
9	8693.0
10	12777.0
11	16861.0
12	16861.0
13	30682.0
14	44503.0
15	70445.0
16	96387.0
17	87372.5
18	78358.0
19	78358.0
20	82541.0
21	86724.0
22	68026.0
23	49328.0
24	54514.0
25	59700.0
26	59700.0
27	64043.0
28	68386.0
29	73684.5
30	78983.0
31	87079.5
32	95176.0
33	95176.0
34	105425.5
35	115675.0
36	127852.5
37	140030.0
38	148927.0
39	157824.0
40	157824.0
41	164962.5
42	172101.0
43	186468.5
44	200836.0
45	214851.5
46	228867.0
47	228867.0
48	294170.0
49	359473.0
50	340159.5
51	320846.0
52	295663.5
53	270481.0
54	270481.0
55	240252.0
56	210023.0
57	180017.5
58	150012.0
59	132627.0
60	115242.0
61	115242.0
62	101372.5
63	87503.0
64	75845.0
65	64187.0
66	55318.5
67	46450.0
68	46450.0
69	40105.5
70	33761.0
71	28667.0
72	23573.0
73	19521.5
74	15470.0
75	15470.0
76	12254.0
77	9038.0
78	7145.0
79	5252.0
80	4069.5
81	2887.0
82	2887.0
83	2213.5
84	1540.0
85	1189.0
86	838.0
87	640.0
88	442.0
89	442.0
90	390.5
91	339.0
92	214.5
93	90.0
94	73.0
95	56.0
96	56.0
97	38.0
98	20.0
99	16.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3427323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.17227839026078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94128487110434	33.64902848549938
2	11.785443619757814	10.17608905809814
3	4.091050265367111	5.298598828949374
4	1.87162007500416	3.232084116755215
5	1.0091061304276454	2.178270539407056
6	0.5983075775376443	1.549818078027463
7	0.41585376569379107	1.2567348179519438
8	0.30175422579436856	1.0421933953145672
9	0.2285444257930224	0.8880105217390792
>10	1.4522608753355464	12.186682446798217
>50	0.16306657197329247	4.9349532142159225
>100	0.12572259062257377	10.678342296557721
>500	0.009753562732047768	2.9045113432974987
>1k	0.005554112111142607	4.657476211725933
>5k	4.0639844715677616E-4	1.4068868459293926
>10k+	2.709322981045174E-4	3.9603197997330275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47373	1.3822157993279303	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38526	1.1240843071983586	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32381	0.944789854939263	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17153	0.5004780699105396	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9045	0.2639085957174156	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8289	0.24185056383655695	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8228	0.2400707490948475	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7948	0.23190110765749244	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7621	0.22236013355029566	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6981	0.20368666740776986	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4987	0.14550714945746288	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4688	0.1367831394940016	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4641	0.13541180682415985	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4398	0.12832172514816959	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4250	0.12400348610271048	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4232	0.12347829486745193	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4052	0.11822638251486656	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3786	0.11046522314937927	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3703	0.10804350800902045	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3555	0.10372526896356137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.9177290847696583E-5	0.0
3	0.0	0.0	0.0	2.9177290847696583E-5	0.0
4	0.0	0.0	0.0	2.9177290847696583E-5	0.0
5	0.0	0.0	0.0	2.9177290847696583E-5	0.0
6	5.8354581695393165E-5	0.0	0.0	2.9177290847696583E-5	0.0
7	5.8354581695393165E-5	0.0	0.0	5.8354581695393165E-5	0.0
8	8.753187254308975E-5	0.0	0.0	8.753187254308975E-5	0.0
9	8.753187254308975E-5	0.0	0.0	2.3341832678157266E-4	0.0
10	1.1670916339078633E-4	0.0	0.0	3.2095019932466243E-4	0.0
11	1.4588645423848292E-4	2.9177290847696583E-5	0.0	5.835458169539317E-4	0.0
12	1.4588645423848292E-4	2.9177290847696583E-5	0.0	9.336733071262906E-4	0.0
13	1.4588645423848292E-4	2.9177290847696583E-5	0.0	0.0010212051796693804	0.0
14	2.042410359338761E-4	2.9177290847696583E-5	0.0	0.001254623506450953	0.0
15	2.042410359338761E-4	2.9177290847696583E-5	0.0	0.0015463964149279189	0.0
16	2.6259561762926925E-4	5.8354581695393165E-5	0.0	0.0021299422318818506	0.0
17	2.6259561762926925E-4	5.8354581695393165E-5	0.0	0.003355388447485107	0.0
18	3.2095019932466243E-4	5.8354581695393165E-5	0.0	0.003997288846134432	0.0
19	3.793047810200556E-4	5.8354581695393165E-5	0.0	0.005018494025803812	0.0
20	3.793047810200556E-4	8.753187254308975E-5	0.0	0.008986605581090547	0.0
21	3.793047810200556E-4	1.1670916339078633E-4	0.0	0.01581409163945155	0.0
22	4.084820718677522E-4	1.1670916339078633E-4	0.0	0.02623038447207923	0.0
23	4.084820718677522E-4	1.1670916339078633E-4	0.0	0.033203756984678715	0.0
24	4.668366535631453E-4	1.1670916339078633E-4	0.0	0.042686376510180105	0.0
25	4.668366535631453E-4	1.750637450861795E-4	0.0	0.05056424503905818	0.0
26	4.96013944410842E-4	1.750637450861795E-4	0.0	0.0615057291069444	0.0
27	4.96013944410842E-4	2.042410359338761E-4	0.0	0.11948100602131752	0.0
28	5.251912352585385E-4	2.3341832678157266E-4	0.0	0.24908653196678573	0.0
29	6.127231078016282E-4	2.3341832678157266E-4	0.0	0.4202405200793739	0.0
30	6.127231078016282E-4	2.3341832678157266E-4	0.0	0.657568603834538	0.0
31	6.127231078016282E-4	2.3341832678157266E-4	0.0	0.9839166019660243	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14185	0.0	20.593233	1
ACGTTTA	170	0.0	18.5	26
GTATTGG	1645	0.0	18.331306	1
TATACCG	190	0.0	17.526316	5
ACCGTTA	685	0.0	16.474453	8
GTATTAG	710	0.0	16.15493	1
GAACCGT	150	4.6802597E-9	16.033333	6
ATTGGAC	2075	0.0	15.245783	3
TACCCCG	440	0.0	15.136364	5
TTGGACC	2560	0.0	15.03125	4
ATTAGAG	600	0.0	14.8	3
TGGACCC	2565	0.0	14.641326	5
TAATACT	835	0.0	14.622754	4
TGTTACG	495	0.0	14.575758	16
TATTGGA	2255	0.0	14.274943	2
AATACTG	870	0.0	14.034482	5
GACCGTT	795	0.0	13.729559	7
GGACCCT	2725	0.0	13.713761	6
TATACAC	1900	0.0	13.53421	37
TCTAGAC	260	1.8189894E-12	13.519231	3
>>END_MODULE