FastQCFastQC Report
Wed 25 May 2016
SRR2088640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215348
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT281471.2705453048460107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT233401.053559079657011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT199070.8985947128848379No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102620.46322293382348956No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58200.2627126753900516No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57510.2595980405787262No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG52380.23644140785104642No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA51570.23278509742036013No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA44910.20272210054582845No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG41430.18701350758436147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.13343276090257603No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28540.1288285181380081No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26540.11980059114865926No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA25760.11627969962281322No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC25580.11546718619377182No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.11311992517654111No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.11262338919212692No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC24890.11235255138244646No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG24830.11208171357276599No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC22480.1014738993602811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79850.021.5234811
TGCTACG450.003826912320.5555552
CTAACGG555.145163E-420.18181827
CTCTAAT3300.017.3787881
GTATTGG12700.016.7519681
ATAACGC1005.8827427E-616.6500023
CTCGTAT803.3843366E-416.18759
TCGTATG855.366939E-415.23529410
TCTAACG1001.094091E-414.82
TGGACCC16850.014.7121665
TTGGACC17350.014.6080694
ATTGGAC14850.014.5757573
TATTGGA15250.014.5573772
TAGAACA2201.8189894E-1114.2954544
CAATGCG1854.9058144E-914.019
GTATCAA123850.013.8918052
TAGATGT4000.013.8750014
CCTATCG800.006301052413.87500113
CAATACT2700.013.7037044
GGACCCT18900.013.6058216
Produced by FastQC (version 0.11.5)