FastQCFastQC Report
Wed 25 May 2016
SRR2088639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403197
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT273731.1390243912588107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236400.9836896434208264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT197440.8215722639467343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106460.44299322943562264No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59990.2496258109509957No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA58100.24176128715207285No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57060.2374337185008137No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG51510.2143394819484212No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA48160.20039971754292302No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG43160.1795940990272541No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA32250.13419623942606454No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.12054775367978572No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.11351545462148963No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG26860.11176778266617343No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26270.10931271968132451No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25690.1068992679335069No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC25520.10619187690397416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87650.020.8956091
TAGACCG450.003827004720.5555555
TAAACGT1301.3951649E-817.0769234
CGCGATA904.4492048E-516.44444514
GTTTAGG3650.016.2191771
GAACCGT700.002593596515.8571436
ACGTTTA1802.0190782E-1015.41666626
GTCTAAT1353.977093E-715.0740751
GTATTAG4850.014.8762881
TTAACGG3000.014.835
TCTAATA6150.014.4390252
TAATACT6550.014.404584
GTATTGG13000.014.2307691
TAACGGC3400.014.14705936
AACGGCC3550.014.07042337
GTATCAA131450.013.9330542
TATACCG1203.303304E-513.87500131
CTAATAC6550.013.8396953
CAACCGT950.001245765513.6315796
TCACGTT1907.1358954E-913.63157924
Produced by FastQC (version 0.11.5)