FastQCFastQC Report
Wed 25 May 2016
SRR2088638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642364
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT349401.3223007882335667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT291831.1044277018609094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238390.9021845589782482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124380.4707148598754751No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG66250.25072245913129304No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG64320.24341839352943045No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA62440.23630355242502546No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA61060.2310809562951963No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA54500.2062547022287618No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG50560.19134381182910457No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.13154887063250936No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32350.12242824985505404No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31790.12030893548352914No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA30810.1166001353333606No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.11652444553437755No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC29010.10978805342488772No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28880.10929606973149801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98400.022.2789651
GTATTGG12650.015.6482221
AGTTCCG3000.014.832
TCTATAC2251.8189894E-1214.83
GTATCAA150100.014.5806132
TAATACT7250.014.54482654
TAGCGAA3600.014.38888810
ACCGTTA4700.014.1702128
TGGACCC18000.014.0805565
CCGTTTA4800.013.87499927
GTAGCGA3750.013.8133339
GTATTAG6700.013.805971
ATTGGAC15300.013.7843133
CGTTTAC5100.013.78431328
GGTAGCG3900.013.7564118
AATACTG7000.013.7428585
TTAGAGT2304.0017767E-1113.6739134
GGACCCT18700.013.6524076
CTAAGGT5850.013.598294
TTGGACC19750.013.5822774
Produced by FastQC (version 0.11.5)