##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2642364
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60383164469392	31.0	31.0	34.0	30.0	34.0
2	31.781647418750786	31.0	31.0	34.0	30.0	34.0
3	31.857100687111995	31.0	31.0	34.0	30.0	34.0
4	35.56082318711578	37.0	35.0	37.0	33.0	37.0
5	35.46747457958101	37.0	35.0	37.0	33.0	37.0
6	35.51009096400042	37.0	35.0	37.0	33.0	37.0
7	35.36864943664083	37.0	35.0	37.0	33.0	37.0
8	35.398765650758186	37.0	35.0	37.0	33.0	37.0
9	36.98653024337298	39.0	37.0	39.0	33.0	39.0
10	36.85028141467262	39.0	37.0	39.0	32.0	39.0
11	36.90078543304405	39.0	37.0	39.0	33.0	39.0
12	36.79617758946156	39.0	37.0	39.0	32.0	39.0
13	36.84312683642375	39.0	37.0	39.0	32.0	39.0
14	37.83528461635112	40.0	37.0	41.0	32.0	41.0
15	37.798698816665684	40.0	37.0	41.0	32.0	41.0
16	37.69726767394651	39.0	37.0	41.0	32.0	41.0
17	37.75612746767667	39.0	37.0	41.0	32.0	41.0
18	37.7404585439402	39.0	37.0	41.0	32.0	41.0
19	37.7738316901078	40.0	37.0	41.0	32.0	41.0
20	37.66011647146267	39.0	37.0	41.0	32.0	41.0
21	37.55986230511769	39.0	37.0	41.0	32.0	41.0
22	37.42182757561032	39.0	36.0	41.0	32.0	41.0
23	37.29177433540572	39.0	36.0	41.0	31.0	41.0
24	37.1511309569764	39.0	36.0	41.0	31.0	41.0
25	36.94982977364209	39.0	36.0	41.0	31.0	41.0
26	37.08079545437343	39.0	36.0	41.0	31.0	41.0
27	36.9995163421845	39.0	36.0	41.0	31.0	41.0
28	36.875057713471726	39.0	35.0	41.0	30.0	41.0
29	36.728933636698045	39.0	35.0	40.0	30.0	41.0
30	36.592344960800254	39.0	35.0	40.0	30.0	41.0
31	36.39582018223076	38.0	35.0	40.0	30.0	41.0
32	36.2787810460633	38.0	35.0	40.0	30.0	41.0
33	36.0687108210678	38.0	35.0	40.0	30.0	41.0
34	35.92790811561163	38.0	35.0	40.0	29.0	41.0
35	35.74413290523183	38.0	35.0	40.0	28.0	41.0
36	35.59336752998451	38.0	35.0	40.0	27.0	41.0
37	35.4149746212104	38.0	34.0	40.0	26.0	41.0
38	35.320710545556935	38.0	34.0	40.0	25.0	41.0
39	35.17072704593311	38.0	34.0	40.0	25.0	41.0
40	34.97983964359187	38.0	34.0	40.0	24.0	41.0
41	34.843031088828035	38.0	34.0	40.0	23.0	41.0
42	34.60981757244649	38.0	33.0	40.0	23.0	41.0
43	34.23416758629772	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	10.0
15	9.0
16	31.0
17	70.0
18	186.0
19	420.0
20	993.0
21	1938.0
22	3641.0
23	6219.0
24	9998.0
25	15500.0
26	23071.0
27	32417.0
28	43620.0
29	56988.0
30	72707.0
31	88597.0
32	108130.0
33	134387.0
34	164282.0
35	203328.0
36	263105.0
37	371933.0
38	497235.0
39	543544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.247080266004225	21.513765703741043	14.468975508294847	22.77017852195988
2	18.890281581190177	23.363473011288377	35.225426928311165	22.520818479210284
3	19.910769295978902	24.70121451851448	32.51845695748201	22.869559228024603
4	13.88362844785957	16.34767200885268	36.05827963142095	33.71041991186679
5	12.875251100908125	38.550139193540325	34.607608944112165	13.967000761439378
6	32.24737394242428	37.18999350581525	13.662387165432166	16.9002453863283
7	25.73827829928049	32.981943441554606	21.738299492424208	19.541478766740692
8	26.839829788780044	34.63440313295216	19.0645951882481	19.461171890019692
9	24.448826883805562	14.57395725948431	22.02751021433837	38.949705642371754
10	16.841775016613912	27.31149077114281	33.180515629186594	22.66621858305669
11	33.04783141156934	22.567178481087392	23.44839696574734	20.936593141595935
12	20.988137894703378	27.262443781401807	30.86300751902463	20.886410804870184
13	31.224464154068098	20.922136389990175	26.50565932626996	21.347740129671763
14	20.44994557903453	22.858054378579183	28.004430880832466	28.68756916155382
15	22.928029597738995	29.369647785089413	25.72268620068999	21.979636416481604
16	21.67116264072626	28.183437255427336	26.871543814553938	23.273856289292468
17	21.699470625545914	26.61264685713248	27.32712071463281	24.360761802688806
18	22.687373881872443	25.419889159858368	29.813152162230487	22.0795847960387
19	22.81082394401377	24.736713034237525	30.828644350286332	21.623818671462374
20	23.609805462078654	24.363940774246093	30.71495827221382	21.311295491461436
21	22.37295845689693	25.301472469349417	31.1263323296866	21.199236744067054
22	21.2904807967411	25.70788884498881	30.666441111065694	22.335189247204397
23	22.08363420028429	25.909110175585198	31.625582243778677	20.381673380351835
24	22.210565993178836	25.991271452381277	29.16642067481997	22.631741879619916
25	22.135670937085123	26.02586169051652	30.285607887482573	21.55285948491578
26	21.8025601317608	26.75816049567736	30.300480932982737	21.138798439579105
27	20.94128590913288	26.80724533031785	29.839908506170985	22.411560254378276
28	21.153860709576726	26.383004007017956	30.249995837061057	22.213139446344258
29	21.75582168088878	26.250357634300194	29.661507649967984	22.332313034843043
30	21.42199182247412	26.386712807168127	31.230443648187762	20.960851722169995
31	21.94951187648636	26.163768504263608	31.05771952690848	20.829000092341555
32	21.58586023727238	25.8591170633569	30.12363171765888	22.43139098171183
33	21.326963279850922	25.96712640650569	30.666895249859593	22.039015063783793
34	20.734690602808698	26.32956700893594	31.15180194704439	21.783940441210977
35	20.380878637462516	28.096961660089224	30.051423649429072	21.47073605301919
36	22.276529652992547	25.875693129334188	30.154399620945487	21.693377596727778
37	21.277121547220595	26.28615890921917	30.198943067647	22.237776475913236
38	21.080441604563184	25.094498714030316	31.52226566816684	22.30279401323966
39	20.503988095508415	25.03247092376372	32.20824231635005	22.255298664377808
40	19.650169318080323	25.821158629166913	31.698017381405442	22.830654671347325
41	20.73775603966751	24.763885672072433	31.834259019574894	22.664099268685163
42	19.624661855823046	26.75657100989871	31.042695101810345	22.576072032467895
43	19.8804555314862	26.472317969817937	30.391270846862884	23.25595565183298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	418.5
2	622.0
3	1556.0
4	2490.0
5	2490.0
6	3818.0
7	5146.0
8	5731.5
9	6317.0
10	9157.5
11	11998.0
12	11998.0
13	22280.5
14	32563.0
15	51108.5
16	69654.0
17	63246.5
18	56839.0
19	56839.0
20	60027.5
21	63216.0
22	49323.0
23	35430.0
24	38889.0
25	42348.0
26	42348.0
27	45996.5
28	49645.0
29	54345.5
30	59046.0
31	65869.5
32	72693.0
33	72693.0
34	82528.5
35	92364.0
36	102710.0
37	113056.0
38	122296.5
39	131537.0
40	131537.0
41	137241.0
42	142945.0
43	155468.5
44	167992.0
45	176755.0
46	185518.0
47	185518.0
48	231808.5
49	278099.0
50	261358.0
51	244617.0
52	225073.0
53	205529.0
54	205529.0
55	182397.5
56	159266.0
57	135864.5
58	112463.0
59	99688.5
60	86914.0
61	86914.0
62	77021.0
63	67128.0
64	58071.0
65	49014.0
66	41817.5
67	34621.0
68	34621.0
69	29300.5
70	23980.0
71	20008.5
72	16037.0
73	13026.5
74	10016.0
75	10016.0
76	7929.5
77	5843.0
78	4587.0
79	3331.0
80	2598.5
81	1866.0
82	1866.0
83	1388.5
84	911.0
85	720.5
86	530.0
87	405.0
88	280.0
89	280.0
90	231.0
91	182.0
92	125.0
93	68.0
94	48.0
95	28.0
96	28.0
97	16.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2642364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.08470135182264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81391578575472	36.78200472706029
2	10.990029043570523	10.129444126416091
3	3.5408561898031246	4.895379001104888
4	1.6073734613909436	2.9630130371618826
5	0.884818309080424	2.0388293762298018
6	0.5560514988214329	1.5375280355651457
7	0.3727556659943238	1.2024833481183714
8	0.30328938866272737	1.1181600719758924
9	0.2088209118272091	0.8661104421814999
>10	1.451258102942994	12.952940815885668
>50	0.1536996447823646	4.9487349324206376
>100	0.10306426525631585	9.132303422671779
>500	0.008802616000441323	2.740064833869143
>1k	0.004442441719848557	3.525009451090127
>5k	4.936046355387285E-4	1.3615589276595257
>10k+	3.2906975702581907E-4	3.8064354505893796
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34940	1.3223007882335667	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29183	1.1044277018609094	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23839	0.9021845589782482	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12438	0.4707148598754751	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6625	0.25072245913129304	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6432	0.24341839352943045	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6244	0.23630355242502546	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6106	0.2310809562951963	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5450	0.2062547022287618	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5056	0.19134381182910457	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3476	0.13154887063250936	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3235	0.12242824985505404	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3179	0.12030893548352914	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3081	0.1166001353333606	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3079	0.11652444553437755	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2901	0.10978805342488772	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2888	0.10929606973149801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.784489949151593E-5	0.0	3.784489949151593E-5	0.0
3	0.0	3.784489949151593E-5	0.0	3.784489949151593E-5	0.0
4	0.0	3.784489949151593E-5	0.0	7.568979898303186E-5	0.0
5	0.0	3.784489949151593E-5	0.0	7.568979898303186E-5	0.0
6	0.0	3.784489949151593E-5	0.0	7.568979898303186E-5	0.0
7	0.0	3.784489949151593E-5	0.0	7.568979898303186E-5	0.0
8	7.568979898303186E-5	3.784489949151593E-5	0.0	7.568979898303186E-5	0.0
9	7.568979898303186E-5	3.784489949151593E-5	0.0	3.784489949151593E-4	0.0
10	1.1353469847454779E-4	3.784489949151593E-5	0.0	7.947428893218346E-4	0.0
11	1.1353469847454779E-4	3.784489949151593E-5	0.0	0.001097502085253962	0.0
12	1.1353469847454779E-4	3.784489949151593E-5	0.0	0.0018165551755927646	0.0
13	1.1353469847454779E-4	3.784489949151593E-5	0.0	0.0019679347735588283	0.0
14	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.002195004170507924	0.0
15	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.0029140572608467264	0.0
16	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.003860179748134625	0.0
17	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.0055632002252528415	0.0
18	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.006357943114574677	0.0
19	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.008439412586608052	0.0
20	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.01627330678135185	0.0
21	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.02724832763389147	0.0
22	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.044316377304565155	0.0
23	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.05445881036829142	0.0
24	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.0691426313709996	0.0
25	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.07833894194743797	0.0
26	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.09116836287506187	0.0
27	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.15247710005131768	0.0
28	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.3024564367361953	0.0
29	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.5043589755234328	0.0
30	1.5137959796606372E-4	3.784489949151593E-5	0.0	0.7997762609542062	0.0
31	1.5137959796606372E-4	3.784489949151593E-5	0.0	1.2226173229729136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9840	0.0	22.278965	1
GTATTGG	1265	0.0	15.648222	1
AGTTCCG	300	0.0	14.8	32
TCTATAC	225	1.8189894E-12	14.8	3
GTATCAA	15010	0.0	14.580613	2
TAATACT	725	0.0	14.5448265	4
TAGCGAA	360	0.0	14.388888	10
ACCGTTA	470	0.0	14.170212	8
TGGACCC	1800	0.0	14.080556	5
CCGTTTA	480	0.0	13.874999	27
GTAGCGA	375	0.0	13.813333	9
GTATTAG	670	0.0	13.80597	1
ATTGGAC	1530	0.0	13.784313	3
CGTTTAC	510	0.0	13.784313	28
GGTAGCG	390	0.0	13.756411	8
AATACTG	700	0.0	13.742858	5
TTAGAGT	230	4.0017767E-11	13.673913	4
GGACCCT	1870	0.0	13.652407	6
CTAAGGT	585	0.0	13.59829	4
TTGGACC	1975	0.0	13.582277	4
>>END_MODULE