FastQCFastQC Report
Wed 25 May 2016
SRR2088637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715250
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133631.8682977979727369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109371.5291156938133519No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91611.280810905277875No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34200.47815449143656064No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG25420.35540020971688224No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA23650.3306536176162181No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA22020.30786438308283814No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19390.27109402306885705No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18700.2614470464872422No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.20706046836770362No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG13710.1916812303390423No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.1660957707095421No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA11590.16204124432016778No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11350.1586857742048235No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9120.12750786438308284No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC8930.1248514505417686No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.12359314924851451No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8270.11562390772457184No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8150.11394617266689969No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG8010.11198881509961552No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7840.10961202376791332No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG7310.1022020272631947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC352.3870563E-531.7142853
TGGTACG400.001930478123.12526
GGTATCA34650.022.3708531
ATCTCGT555.1404844E-420.1818187
CTCGTAT609.232165E-418.59
TCGTATG701.2183804E-418.510
GGTACGT500.007032349718.49999827
GACGTTT500.007032349718.49999823
GTACGTG500.007032349718.49999828
TCGCTAC852.7207876E-517.41176427
TCTAATA2050.017.1463412
TTCGTTA650.001579145617.07692330
TCTCACG650.001579145617.07692317
GGTAAAC1554.0017767E-1016.70967935
TATTAAC1005.8715505E-616.652
TCCAGAC904.442179E-516.4444453
GCTCGGA1352.2149834E-816.44444511
TAGTACT904.442179E-516.4444454
GTACTGC1708.54925E-1116.323536
TGTACTG1951.8189894E-1216.1282065
Produced by FastQC (version 0.11.5)