##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	715250
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.548325760223698	31.0	31.0	34.0	30.0	34.0
2	31.72955609926599	31.0	31.0	34.0	30.0	34.0
3	31.774438308283816	31.0	31.0	34.0	30.0	34.0
4	35.50507235232436	37.0	35.0	37.0	33.0	37.0
5	35.42144425026215	37.0	35.0	37.0	33.0	37.0
6	35.4637119888151	37.0	35.0	37.0	33.0	37.0
7	35.325796574624256	37.0	35.0	37.0	32.0	37.0
8	35.36553093324012	37.0	35.0	37.0	33.0	37.0
9	36.94473680531283	39.0	37.0	39.0	33.0	39.0
10	36.82585669346383	39.0	37.0	39.0	32.0	39.0
11	36.85676197133869	39.0	37.0	39.0	32.0	39.0
12	36.7476392869626	39.0	37.0	39.0	32.0	39.0
13	36.79670464872422	39.0	37.0	39.0	32.0	39.0
14	37.71069556099266	40.0	37.0	41.0	32.0	41.0
15	37.67958476057323	39.0	37.0	41.0	32.0	41.0
16	37.56673890248165	39.0	36.0	41.0	32.0	41.0
17	37.68016218105557	39.0	37.0	41.0	32.0	41.0
18	37.69392380286613	39.0	37.0	41.0	32.0	41.0
19	37.755665851101014	40.0	37.0	41.0	32.0	41.0
20	37.64359314924852	39.0	37.0	41.0	32.0	41.0
21	37.54917441454037	39.0	36.0	41.0	32.0	41.0
22	37.40455505068158	39.0	36.0	41.0	32.0	41.0
23	37.27228102062216	39.0	36.0	41.0	31.0	41.0
24	37.120588605382736	39.0	36.0	41.0	31.0	41.0
25	36.923630898287314	39.0	36.0	41.0	31.0	41.0
26	37.02996714435512	39.0	36.0	41.0	31.0	41.0
27	36.93846207619713	39.0	35.0	41.0	31.0	41.0
28	36.791605732261445	39.0	35.0	41.0	30.0	41.0
29	36.61992590003495	39.0	35.0	40.0	30.0	41.0
30	36.47100034952814	39.0	35.0	40.0	30.0	41.0
31	36.22275847605732	38.0	35.0	40.0	30.0	41.0
32	36.08376092275428	38.0	35.0	40.0	30.0	41.0
33	35.85640685075148	38.0	35.0	40.0	29.0	41.0
34	35.69363579168123	38.0	35.0	40.0	28.0	41.0
35	35.47722894092974	38.0	34.0	40.0	27.0	41.0
36	35.31525900034953	38.0	34.0	40.0	26.0	41.0
37	35.12373296050332	38.0	34.0	40.0	25.0	41.0
38	35.00314155889549	38.0	34.0	40.0	24.0	41.0
39	34.8422341838518	38.0	34.0	40.0	24.0	41.0
40	34.60526529185599	38.0	33.0	40.0	23.0	41.0
41	34.452078294302694	38.0	33.0	40.0	22.0	41.0
42	34.17318280321566	38.0	33.0	40.0	20.0	41.0
43	33.78633205173016	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	6.0
15	4.0
16	18.0
17	20.0
18	60.0
19	103.0
20	283.0
21	585.0
22	1100.0
23	1903.0
24	3158.0
25	4706.0
26	6757.0
27	9528.0
28	13090.0
29	16562.0
30	20841.0
31	24549.0
32	30262.0
33	37033.0
34	44875.0
35	55324.0
36	72088.0
37	102706.0
38	130185.0
39	139503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.621950367004544	22.36602586508214	16.948199930094372	21.063823837818944
2	18.417756029360362	23.491506466270533	37.166165676336945	20.924571828032157
3	18.90024466969591	25.364557846906678	34.090457881859486	21.644739601537925
4	14.003495281370151	17.5698007689619	35.25480601188396	33.17189793778399
5	12.633904229290458	38.479972037749036	34.47116392869626	14.414959804264244
6	30.383921705697308	38.160782943026916	13.76903180706047	17.686263544215308
7	23.89234533379937	33.96001398112548	21.906606081789583	20.241034603285566
8	25.563928696260046	35.42411744145404	19.17818944425026	19.833764418035653
9	23.23439356868228	15.550786438308284	22.917022020272633	38.29779797273681
10	17.860048933939183	27.43795875567983	32.54861936385879	22.153372946522197
11	31.792520097867875	23.571758126529186	23.364837469416287	21.270884306186648
12	21.274938832576023	28.769521146452288	30.027403005941977	19.92813701502971
13	31.78217406501223	21.37140859839217	26.830059419783293	20.016357916812304
14	20.00880810905278	24.11981824536875	28.84208318769661	27.02929045788186
15	22.468926948619362	29.86927647675638	26.437329605033206	21.224466969591052
16	20.593918210415936	28.061516952114644	28.030618664802514	23.3139461726669
17	21.445508563439354	26.660747990213213	27.96183152743796	23.93191191890947
18	22.472841663753933	24.671094023068857	31.08060118839567	21.775463124781545
19	22.73974134917861	24.230548759175115	32.50625655365257	20.523453337993708
20	23.09262495630898	23.873610625655363	32.51506466270534	20.518699755330307
21	22.09325410695561	24.765047186298496	33.04397063963649	20.0977280671094
22	20.36672492135617	25.449702901083537	32.51198881509961	21.671583362460677
23	21.623068857042995	25.441034603285566	33.711988815099616	19.223907724571827
24	21.507305138063614	25.736176162181057	31.095421181405104	21.66109751835023
25	21.459210066410346	25.686822789234533	32.037189793778396	20.81677735057672
26	20.93226144704649	26.679063264592802	32.30968192939532	20.0789933589654
27	19.871094023068856	26.514365606431316	31.98671793079343	21.627822439706396
28	20.390073400908772	26.41468018175463	31.85207969241524	21.343166724921357
29	20.63698007689619	26.127368053128276	31.140999650471862	22.09465221950367
30	20.76700454386578	26.1511359664453	32.93981125480601	20.14204823488291
31	21.398531981824537	25.582243970639635	32.999650471862985	20.01957357567284
32	20.71723173715484	25.606151695211466	31.928276826284517	21.74833974134918
33	20.296819293953163	25.679133170220204	32.4883607130374	21.535686822789234
34	19.89947570779448	25.98504019573576	33.06144704648724	21.054037049982526
35	19.46564138413142	28.09884655714785	31.767074449493183	20.668437609227546
36	21.42649423278574	25.661377140859837	31.856693463823838	21.055435162530582
37	20.32883607130374	26.544145403705	31.784690667598742	21.342327857392522
38	20.466410346032855	25.048724222299896	33.1440754980776	21.340789933589654
39	19.613282069206573	25.186578119538623	33.929255505068156	21.270884306186648
40	18.491017126878713	26.32506116742398	33.350297098916464	21.833624606780848
41	19.989933589653965	25.23914715134568	33.06550157287661	21.70541768612373
42	18.83998601887452	27.362041244320167	32.08500524292205	21.71296749388326
43	19.15442153093324	27.44592799720377	30.910031457532334	22.489619014330653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59.0
1	122.0
2	185.0
3	468.0
4	751.0
5	751.0
6	1149.0
7	1547.0
8	1805.5
9	2064.0
10	3016.5
11	3969.0
12	3969.0
13	7058.0
14	10147.0
15	17525.0
16	24903.0
17	22480.0
18	20057.0
19	20057.0
20	20853.5
21	21650.0
22	16393.5
23	11137.0
24	11931.5
25	12726.0
26	12726.0
27	13702.0
28	14678.0
29	15922.0
30	17166.0
31	18827.5
32	20489.0
33	20489.0
34	22614.5
35	24740.0
36	27465.0
37	30190.0
38	32335.0
39	34480.0
40	34480.0
41	35788.5
42	37097.0
43	40311.0
44	43525.0
45	46998.5
46	50472.0
47	50472.0
48	65037.5
49	79603.0
50	73363.5
51	67124.0
52	60753.0
53	54382.0
54	54382.0
55	47249.0
56	40116.0
57	33694.0
58	27272.0
59	23586.5
60	19901.0
61	19901.0
62	17414.0
63	14927.0
64	12659.5
65	10392.0
66	8750.0
67	7108.0
68	7108.0
69	5983.0
70	4858.0
71	4068.5
72	3279.0
73	2598.5
74	1918.0
75	1918.0
76	1513.5
77	1109.0
78	858.5
79	608.0
80	448.0
81	288.0
82	288.0
83	220.5
84	153.0
85	114.0
86	75.0
87	60.0
88	45.0
89	45.0
90	39.0
91	33.0
92	22.5
93	12.0
94	11.0
95	10.0
96	10.0
97	7.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	715250.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.038283873696116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65621079225126	46.287146343368256
2	7.643514049442689	8.26084763992737
3	2.295930801796994	3.7220448126560606
4	1.0955807973739022	2.368132245402451
5	0.652556554386717	1.7631518164795215
6	0.4438873684140156	1.4392146973382702
7	0.3049194198721708	1.1534125518758571
8	0.27008312137135443	1.1675862705727336
9	0.19906385642253613	0.9681362264118322
>10	1.255127388609293	12.928114213787484
>50	0.1144860654625922	4.344357579132103
>100	0.059833170723761465	6.1023646205264646
>500	0.0051803611016121584	1.918553875256841
>1k	0.002849198605886687	2.8934373465608383
>5k	2.590180550806079E-4	1.2822552018113313
>10k+	5.180361101612158E-4	3.4012445588926266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13363	1.8682977979727369	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10937	1.5291156938133519	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9161	1.280810905277875	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3420	0.47815449143656064	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2542	0.35540020971688224	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2365	0.3306536176162181	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2202	0.30786438308283814	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1939	0.27109402306885705	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1870	0.2614470464872422	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.20706046836770362	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1371	0.1916812303390423	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1188	0.1660957707095421	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1159	0.16204124432016778	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1135	0.1586857742048235	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	912	0.12750786438308284	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	893	0.1248514505417686	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.12359314924851451	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	827	0.11562390772457184	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	815	0.11394617266689969	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	801	0.11198881509961552	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	784	0.10961202376791332	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	731	0.1022020272631947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.398112548060119E-4	0.0
9	0.0	0.0	0.0	8.388675288360713E-4	0.0
10	0.0	0.0	0.0	0.0018175463124781544	0.0
11	1.398112548060119E-4	0.0	0.0	0.0030758476057322613	0.0
12	1.398112548060119E-4	0.0	0.0	0.0047535826634044044	0.0
13	1.398112548060119E-4	0.0	0.0	0.005592450192240475	0.0
14	2.796225096120238E-4	0.0	0.0	0.006011883956658511	0.0
15	2.796225096120238E-4	0.0	0.0	0.008947920307584761	0.0
16	2.796225096120238E-4	0.0	0.0	0.01048584411045089	0.0
17	2.796225096120238E-4	0.0	0.0	0.014959804264243272	0.0
18	2.796225096120238E-4	0.0	0.0	0.018035651869975532	0.0
19	2.796225096120238E-4	0.0	0.0	0.022090178259349877	0.0
20	2.796225096120238E-4	0.0	0.0	0.03467319119189095	0.0
21	2.796225096120238E-4	0.0	0.0	0.050611674239776305	0.0
22	2.796225096120238E-4	0.0	0.0	0.08220901782593498	0.0
23	2.796225096120238E-4	0.0	0.0	0.10555749737853898	0.0
24	2.796225096120238E-4	0.0	0.0	0.13337993708493534	0.0
25	2.796225096120238E-4	0.0	0.0	0.15001747640685076	0.0
26	2.796225096120238E-4	0.0	0.0	0.1729465221950367	0.0
27	2.796225096120238E-4	0.0	0.0	0.258790632645928	0.0
28	2.796225096120238E-4	0.0	0.0	0.5152044739601538	0.0
29	2.796225096120238E-4	0.0	0.0	0.882348829080741	0.0
30	4.1943376441803563E-4	0.0	0.0	1.375183502271933	0.0
31	5.592450192240476E-4	0.0	0.0	1.9923103809856693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	35	2.3870563E-5	31.714285	3
TGGTACG	40	0.0019304781	23.125	26
GGTATCA	3465	0.0	22.370853	1
ATCTCGT	55	5.1404844E-4	20.181818	7
CTCGTAT	60	9.232165E-4	18.5	9
TCGTATG	70	1.2183804E-4	18.5	10
GGTACGT	50	0.0070323497	18.499998	27
GACGTTT	50	0.0070323497	18.499998	23
GTACGTG	50	0.0070323497	18.499998	28
TCGCTAC	85	2.7207876E-5	17.411764	27
TCTAATA	205	0.0	17.146341	2
TTCGTTA	65	0.0015791456	17.076923	30
TCTCACG	65	0.0015791456	17.076923	17
GGTAAAC	155	4.0017767E-10	16.709679	35
TATTAAC	100	5.8715505E-6	16.65	2
TCCAGAC	90	4.442179E-5	16.444445	3
GCTCGGA	135	2.2149834E-8	16.444445	11
TAGTACT	90	4.442179E-5	16.444445	4
GTACTGC	170	8.54925E-11	16.32353	6
TGTACTG	195	1.8189894E-12	16.128206	5
>>END_MODULE