FastQCFastQC Report
Wed 25 May 2016
SRR2088636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616212
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78271.2701797433350859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76011.2335040537996662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42230.6853160925136155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.4608803463742998No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG19180.311256515614756No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG15450.2507253997001032No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA14710.23871654560443484No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA10930.17737402062926397No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA9530.15465456693475624No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.14264571283908786No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.14086061290594795No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG8460.13729041303966816No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC7360.11943941370826923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.025.9397871
GTAAGAT806.94934E-720.81253
TAGACAG909.460382E-820.5555575
TTTATAC909.460382E-820.5555573
TAGACTG555.139372E-420.1818185
GATAGAC555.139372E-420.1818183
TTAGACT656.895492E-519.9230774
TACACTG1052.2542736E-819.380955
GTATCAA32100.018.730532
GGGTAAG1205.160473E-918.51
ACGGGTA701.2180328E-418.55
CTTCTAA500.0070312718.4999981
GGTAAGA1156.3906555E-817.6956522
GATTTGC953.600897E-617.5263167
AGTTCCT1005.868893E-616.6516
ATAGACA904.4405726E-516.4444454
GCTTCGT904.4405726E-516.44444528
GACAGCG803.3793817E-416.18757
CAGTACT1151.2398323E-616.0869564
CGCGGTA700.00259066415.85714312
Produced by FastQC (version 0.11.5)