##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	616212
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81852998643324	31.0	31.0	31.0	28.0	34.0
2	30.991785294671313	31.0	31.0	33.0	28.0	34.0
3	30.943110487948953	31.0	31.0	34.0	27.0	34.0
4	34.72099861735896	35.0	35.0	37.0	32.0	37.0
5	34.48369392351983	35.0	35.0	37.0	30.0	37.0
6	34.71638981389522	35.0	35.0	37.0	32.0	37.0
7	34.61545864085737	35.0	35.0	37.0	31.0	37.0
8	34.6821288777239	35.0	35.0	37.0	31.0	37.0
9	36.15631146423633	38.0	35.0	39.0	32.0	39.0
10	35.84608706094656	37.0	35.0	39.0	30.0	39.0
11	36.027884883773766	37.0	35.0	39.0	31.0	39.0
12	35.80785671165118	37.0	35.0	39.0	30.0	39.0
13	35.94676669717565	37.0	35.0	39.0	30.0	39.0
14	36.70723711969257	38.0	36.0	40.0	31.0	41.0
15	36.696810513264914	38.0	36.0	40.0	31.0	41.0
16	36.520442639870694	38.0	35.0	40.0	30.0	41.0
17	36.57847137024271	38.0	35.0	40.0	30.0	41.0
18	36.562989685368024	38.0	35.0	40.0	30.0	41.0
19	36.580441471441645	38.0	35.0	40.0	30.0	41.0
20	36.45027360713521	38.0	35.0	40.0	30.0	41.0
21	36.317049976306855	38.0	35.0	40.0	30.0	41.0
22	36.145354196283094	38.0	34.0	40.0	29.0	41.0
23	35.98971295593075	38.0	34.0	40.0	29.0	41.0
24	35.83116687114175	38.0	34.0	40.0	27.0	41.0
25	35.60080783886065	38.0	34.0	40.0	27.0	41.0
26	35.686836673093026	38.0	34.0	40.0	27.0	41.0
27	35.57768917190837	38.0	34.0	40.0	27.0	41.0
28	35.42162762166267	38.0	34.0	40.0	27.0	41.0
29	35.24081160379869	38.0	34.0	40.0	27.0	41.0
30	35.13028470721115	38.0	33.0	40.0	26.0	41.0
31	34.92426470110936	37.0	33.0	40.0	25.0	41.0
32	34.78378382764373	37.0	33.0	40.0	25.0	41.0
33	34.546625187435495	37.0	33.0	40.0	25.0	41.0
34	34.374944012774826	37.0	33.0	40.0	24.0	41.0
35	34.171994378558026	37.0	33.0	40.0	24.0	41.0
36	34.01914763101011	37.0	33.0	40.0	24.0	41.0
37	33.84374208876166	37.0	32.0	40.0	23.0	41.0
38	33.73552608517848	37.0	32.0	40.0	22.0	41.0
39	33.545578469747426	37.0	32.0	40.0	21.0	41.0
40	33.33962824482483	37.0	31.0	40.0	20.0	41.0
41	33.17929381446645	37.0	31.0	39.0	19.0	41.0
42	32.943665491746344	36.0	31.0	39.0	18.0	41.0
43	32.53961623597074	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	4.0
15	11.0
16	45.0
17	64.0
18	154.0
19	317.0
20	630.0
21	1199.0
22	1887.0
23	3026.0
24	4680.0
25	6497.0
26	9010.0
27	12068.0
28	15378.0
29	19529.0
30	23787.0
31	28983.0
32	34840.0
33	41577.0
34	49514.0
35	61132.0
36	77538.0
37	92493.0
38	90338.0
39	41505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.384497543053364	24.776051099296996	15.388697396350606	20.450753961299036
2	23.8276761893634	25.037811662220143	30.37006744432111	20.764444704095343
3	24.42373728522002	24.635515050015254	30.550687101192448	20.390060563572277
4	19.101705257281584	18.903559164703058	32.17415434947713	29.820581228538227
5	13.209577223423107	40.08490584409262	33.649133739686995	13.056383192797282
6	32.96787469247597	37.428514861768356	13.001369658494156	16.602240787261525
7	25.91786592925811	33.24635028204579	21.210070560131904	19.625713228564194
8	27.421406918398212	35.53403698727061	18.2789364699162	18.765619624414974
9	24.953262838114153	14.043868019447853	21.063692365614433	39.93917677682356
10	17.25688561728756	28.252452078180884	32.62805657793097	21.862605726600588
11	34.79451876951439	22.115116226233827	22.355131026335094	20.735233977916693
12	20.30827053027205	27.638053137556557	30.913873796680363	21.139802535491032
13	32.34893186111273	20.491162132512837	26.05548090592199	21.104425100452442
14	20.343323401686433	22.6597339876536	27.666127891050486	29.33081471960948
15	23.736149247336954	29.414876698279162	24.948394383751047	21.900579670632833
16	22.245590803165143	27.87676968316099	26.82826689515946	23.049372618514408
17	22.049716655956068	26.968153817192785	27.313814076973507	23.66831544987764
18	22.805300773110552	25.263707944668397	29.01566344050424	22.915327841716813
19	23.26066353787333	24.681116239216376	30.42199762419427	21.636222598716024
20	23.760329237340397	24.268920436473163	30.662174706107635	21.308575620078805
21	22.94697279507702	25.09931646900742	30.849934762711534	21.103775973204026
22	21.81765366464788	25.440432838049244	30.504761348367122	22.237152148935756
23	22.38060927083536	25.5994690139108	31.37021025231576	20.64971146293808
24	22.62565480711184	25.828935496225323	29.04698383024024	22.498425866422593
25	22.791019973645437	26.036818497530074	29.558496101990873	21.613665426833624
26	22.38060927083536	26.48617683524501	29.872349126599286	21.260864767320335
27	22.049229810519755	26.098647867941555	29.599553400453093	22.2525689210856
28	21.83777660934873	26.25103048950686	30.14060096200658	21.77059193913783
29	22.135726016370988	25.694241592179313	29.974748950036677	22.195283441413018
30	22.273827838471174	25.91332203851921	30.67353443295489	21.139315690054723
31	22.52455323817128	25.843703141126756	30.38808072546461	21.243662895237353
32	22.345394117608876	25.652210602844477	29.80727411994573	22.195121159600916
33	22.1943097505404	25.95616443691457	29.99730612191908	21.852219690625954
34	21.711196795907902	26.127858594120205	30.589634736097317	21.571309873874576
35	21.293483411553165	27.425950809137117	29.78747573886909	21.493090040440627
36	23.14398291497082	25.36334897730002	29.89798965291166	21.594678454817497
37	22.068379064347983	26.10254263143204	29.77400634846449	22.055071955755487
38	22.086230063679384	24.847130532998385	30.980247057830745	22.086392345491486
39	21.37154096317501	24.839503287829512	31.45978981259696	22.329165936398514
40	20.881449890622058	25.657403620831793	30.658117660805047	22.8030288277411
41	21.74105664933497	24.761283454395567	30.863079589491925	22.634580306777536
42	21.069209947225957	26.14587187526371	30.046315229174375	22.738602948335963
43	21.493901449501145	25.80053617910719	29.59776830051995	23.107794070871712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	70.5
2	96.0
3	174.5
4	253.0
5	253.0
6	507.0
7	761.0
8	894.0
9	1027.0
10	1617.5
11	2208.0
12	2208.0
13	4706.0
14	7204.0
15	11542.0
16	15880.0
17	13840.0
18	11800.0
19	11800.0
20	13595.0
21	15390.0
22	11938.5
23	8487.0
24	9074.0
25	9661.0
26	9661.0
27	10464.0
28	11267.0
29	12600.0
30	13933.0
31	15356.5
32	16780.0
33	16780.0
34	19110.0
35	21440.0
36	23777.0
37	26114.0
38	28219.5
39	30325.0
40	30325.0
41	31821.5
42	33318.0
43	35483.0
44	37648.0
45	39624.0
46	41600.0
47	41600.0
48	51826.5
49	62053.0
50	58458.0
51	54863.0
52	50655.0
53	46447.0
54	46447.0
55	41703.0
56	36959.0
57	32824.5
58	28690.0
59	25709.0
60	22728.0
61	22728.0
62	20308.0
63	17888.0
64	15545.0
65	13202.0
66	11252.5
67	9303.0
68	9303.0
69	8035.5
70	6768.0
71	5770.0
72	4772.0
73	3933.0
74	3094.0
75	3094.0
76	2508.5
77	1923.0
78	1496.0
79	1069.0
80	809.5
81	550.0
82	550.0
83	433.5
84	317.0
85	236.5
86	156.0
87	133.0
88	110.0
89	110.0
90	81.5
91	53.0
92	35.0
93	17.0
94	12.0
95	7.0
96	7.0
97	5.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	616212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.87221703851628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20112602858508	53.953269956436266
2	7.041232576663689	8.71313340404014
3	2.1287365639589417	3.9512895210927907
4	0.9454652429559649	2.3399212285818
5	0.5735430127614849	1.7743188883251555
6	0.39031191158132017	1.4489677983646574
7	0.2829016953855152	1.2252628568219772
8	0.20562970218177493	1.0178212450364996
9	0.15899806474547676	0.8853806493572581
>10	0.9544662908747531	11.104315338581367
>50	0.07060585331830126	2.9645986368676804
>100	0.042783454128518854	5.009366100726009
>500	0.002099801429619724	0.9810536411359374
>1k	0.001574851072214793	2.12580568820881
>5k	5.24950357404931E-4	2.5054950464236456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7827	1.2701797433350859	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7601	1.2335040537996662	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4223	0.6853160925136155	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2840	0.4608803463742998	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1918	0.311256515614756	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1545	0.2507253997001032	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1471	0.23871654560443484	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1093	0.17737402062926397	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	953	0.15465456693475624	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	879	0.14264571283908786	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	868	0.14086061290594795	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	846	0.13729041303966816	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	736	0.11943941370826923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.6228181210362668E-4	0.0	0.0	0.0	0.0
6	1.6228181210362668E-4	0.0	0.0	0.0	0.0
7	1.6228181210362668E-4	0.0	0.0	0.0	0.0
8	1.6228181210362668E-4	0.0	0.0	0.0	0.0
9	1.6228181210362668E-4	0.0	1.6228181210362668E-4	1.6228181210362668E-4	0.0
10	1.6228181210362668E-4	0.0	1.6228181210362668E-4	3.2456362420725337E-4	0.0
11	1.6228181210362668E-4	0.0	1.6228181210362668E-4	3.2456362420725337E-4	0.0
12	1.6228181210362668E-4	0.0	1.6228181210362668E-4	8.114090605181334E-4	0.0
13	1.6228181210362668E-4	0.0	1.6228181210362668E-4	8.114090605181334E-4	0.0
14	1.6228181210362668E-4	0.0	1.6228181210362668E-4	8.114090605181334E-4	0.0
15	1.6228181210362668E-4	0.0	3.2456362420725337E-4	0.0011359726847253867	0.0
16	1.6228181210362668E-4	0.0	3.2456362420725337E-4	0.0016228181210362668	0.0
17	1.6228181210362668E-4	0.0	3.2456362420725337E-4	0.00194738174524352	0.0
18	1.6228181210362668E-4	0.0	3.2456362420725337E-4	0.002596508993658027	0.0
19	1.6228181210362668E-4	0.0	3.2456362420725337E-4	0.004219327114694293	0.0
20	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.007627245168870454	0.0
21	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.010710599598839361	0.0
22	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.018662408391917067	0.0
23	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.023530862755025868	0.0
24	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.033754616917554346	0.0
25	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.03862307128066315	0.0
26	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.04397837108008283	0.0
27	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.08536023316650763	0.0
28	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.17477751163560593	0.0
29	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.2896730346049736	0.0
30	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.44432760153972983	0.0
31	1.6228181210362668E-4	0.0	4.8684543631088E-4	0.6791493836536776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2325	0.0	25.939787	1
GTAAGAT	80	6.94934E-7	20.8125	3
TAGACAG	90	9.460382E-8	20.555557	5
TTTATAC	90	9.460382E-8	20.555557	3
TAGACTG	55	5.139372E-4	20.181818	5
GATAGAC	55	5.139372E-4	20.181818	3
TTAGACT	65	6.895492E-5	19.923077	4
TACACTG	105	2.2542736E-8	19.38095	5
GTATCAA	3210	0.0	18.73053	2
GGGTAAG	120	5.160473E-9	18.5	1
ACGGGTA	70	1.2180328E-4	18.5	5
CTTCTAA	50	0.00703127	18.499998	1
GGTAAGA	115	6.3906555E-8	17.695652	2
GATTTGC	95	3.600897E-6	17.526316	7
AGTTCCT	100	5.868893E-6	16.65	16
ATAGACA	90	4.4405726E-5	16.444445	4
GCTTCGT	90	4.4405726E-5	16.444445	28
GACAGCG	80	3.3793817E-4	16.1875	7
CAGTACT	115	1.2398323E-6	16.086956	4
CGCGGTA	70	0.002590664	15.857143	12
>>END_MODULE