FastQCFastQC Report
Wed 25 May 2016
SRR2088635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721927
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.9901278107066227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64110.8880399264745604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51530.7137840806618951No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.37524569658705104No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14070.1948950517157552No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14030.1943409790740615No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG13730.19018543426135884No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG13280.18395211704230482No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA12590.1743943639730887No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG10810.14973813141771952No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.10749009248857572No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.10596639272391806No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7560.10471972928010725No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA7450.10319602951544962No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.10042566630698117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG551.9013882E-523.54545421
ATTAGAC603.7238147E-521.5833343
GGTATCA23900.021.3640171
GACAGTA450.003824449120.5555577
GACCGTC450.003824449120.55555722
ACCGTCG450.003824449120.55555723
GGCGTCG555.1405473E-420.1818188
GTCGTCA656.8975874E-519.92307724
TAGTGTC656.8975874E-519.92307719
GTATAGA759.257945E-619.7333341
TACCCGA1457.2759576E-1219.1379311
TTCGCTA1551.8189894E-1219.09677526
GGGCGTC701.2184004E-418.57
TATACTG801.6153132E-518.55
TCTATAC609.232278E-418.53
GGCTAAC500.007032410718.4999981
CGAAATT1156.395021E-817.69565213
TTACCCG1603.45608E-1117.3437510
TGTGCGC752.0659246E-417.26666610
TCCTACA752.0659246E-417.2666662
Produced by FastQC (version 0.11.5)