##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721927
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.774003465724373	31.0	31.0	34.0	30.0	34.0
2	31.96413349272156	31.0	31.0	34.0	30.0	34.0
3	32.04432442615389	33.0	31.0	34.0	30.0	34.0
4	35.720193315944684	37.0	35.0	37.0	35.0	37.0
5	35.63157909317701	37.0	35.0	37.0	33.0	37.0
6	35.662936834333664	37.0	35.0	37.0	33.0	37.0
7	35.539866219160665	37.0	35.0	37.0	33.0	37.0
8	35.55582074087823	37.0	35.0	37.0	33.0	37.0
9	37.182172158680864	39.0	37.0	39.0	33.0	39.0
10	37.045850896281756	39.0	37.0	39.0	33.0	39.0
11	37.095526278972805	39.0	37.0	39.0	33.0	39.0
12	36.99788898323515	39.0	37.0	39.0	33.0	39.0
13	37.0344162221388	39.0	37.0	39.0	33.0	39.0
14	38.09320609978571	40.0	37.0	41.0	33.0	41.0
15	38.05915002486401	40.0	37.0	41.0	33.0	41.0
16	37.96133542588101	40.0	37.0	41.0	33.0	41.0
17	37.977115414716444	40.0	37.0	41.0	33.0	41.0
18	37.948124948921425	40.0	37.0	41.0	33.0	41.0
19	37.97254985614889	40.0	37.0	41.0	33.0	41.0
20	37.859772525476956	40.0	37.0	41.0	32.0	41.0
21	37.75277001691307	40.0	37.0	41.0	32.0	41.0
22	37.60517060589228	39.0	37.0	41.0	32.0	41.0
23	37.48108742296659	39.0	36.0	41.0	32.0	41.0
24	37.3393431745869	39.0	36.0	41.0	31.0	41.0
25	37.147790566082165	39.0	36.0	41.0	31.0	41.0
26	37.309222400602835	39.0	36.0	41.0	31.0	41.0
27	37.24560377988356	39.0	36.0	41.0	31.0	41.0
28	37.13092459486901	39.0	36.0	41.0	31.0	41.0
29	36.99425980743205	39.0	36.0	41.0	31.0	41.0
30	36.874785123703646	39.0	35.0	41.0	30.0	41.0
31	36.70203912583959	39.0	35.0	41.0	30.0	41.0
32	36.58690837162206	39.0	35.0	40.0	30.0	41.0
33	36.388366136742356	39.0	35.0	40.0	30.0	41.0
34	36.253118390086534	38.0	35.0	40.0	30.0	41.0
35	36.07220536148392	38.0	35.0	40.0	29.0	41.0
36	35.93867108447253	38.0	35.0	40.0	29.0	41.0
37	35.781887919415674	38.0	35.0	40.0	27.0	41.0
38	35.705657220189856	38.0	35.0	40.0	27.0	41.0
39	35.58464082933593	38.0	35.0	40.0	26.0	41.0
40	35.417133588299095	38.0	35.0	40.0	25.0	41.0
41	35.286772762343006	38.0	34.0	40.0	25.0	41.0
42	35.079678416238764	38.0	34.0	40.0	24.0	41.0
43	34.71671373975485	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	2.0
15	5.0
16	4.0
17	22.0
18	55.0
19	94.0
20	225.0
21	408.0
22	805.0
23	1441.0
24	2330.0
25	3650.0
26	5242.0
27	7557.0
28	10357.0
29	13748.0
30	17502.0
31	21990.0
32	27004.0
33	34001.0
34	42390.0
35	53415.0
36	70238.0
37	100650.0
38	140406.0
39	168384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7412009801545	21.022070098500265	13.60483816230727	23.63189075903796
2	18.954963590501535	22.45753379496819	35.352466385105416	23.235036229424857
3	19.80172510516991	24.448455314734037	32.0157024186656	23.734117161430447
4	13.895172226554761	16.203992924492365	36.19382569151728	33.707009157435586
5	13.20978436877967	38.00204175768464	34.88995424745161	13.898219626084076
6	32.72366873658971	36.875612077121374	13.978560159129664	16.422159027159257
7	26.34560003989323	32.307560182677754	22.02244825307822	19.3243915243508
8	26.55102247180117	34.72220875517885	19.181163746472983	19.545605026547005
9	24.678533979197343	14.58236082041536	21.572402749862523	39.166702450524774
10	16.70196571121457	26.872800158464777	33.773913428920096	22.651320701400557
11	33.41154992125242	22.514741795223063	23.221184413382517	20.852523870141994
12	20.99963015651167	26.793013698060886	30.858660224648755	21.34869592077869
13	30.8630928057823	20.884521565199808	26.034211215261376	22.21817441375652
14	20.657490300265817	22.768230028797927	27.414821720201626	29.15945795073463
15	23.336154486533957	29.14602168917356	24.99934203873799	22.518481785554496
16	22.307518627229623	27.95989068146779	26.427879827184743	23.30471086411784
17	22.017323081142553	26.68081398811791	27.059661156875976	24.242201773863563
18	22.876551230249042	25.589152365820922	29.068174483015596	22.466121920914443
19	23.005788673924094	25.16210087723551	29.84096730001787	21.991143148822527
20	23.810302149663332	24.764138202339016	29.662555909392502	21.76300373860515
21	22.746067123130178	25.574607958976465	29.971035852655465	21.7082890652379
22	21.85775016033477	25.969661752504063	29.506861497076574	22.665726590084592
23	22.366250327249155	26.090588106553707	30.429946518138262	21.113215048058876
24	22.599376391241773	26.341998567722218	28.296628329457135	22.76199671157887
25	22.550756516933152	26.1508435063379	29.248386609726467	22.05001336700248
26	22.258483198439734	26.863519441716406	29.260853244164576	21.617144115679285
27	21.649834401539213	26.820163257503875	28.87701942163127	22.652982919325638
28	21.79610957894635	26.452259023419266	29.200736362540813	22.550895035093575
29	22.16816935784366	26.533846219908664	28.752076040929346	22.545908381318334
30	21.972443197165365	26.661837000139904	30.02976755267499	21.335952250019737
31	22.395339140938074	26.362083700983618	29.897759745791475	21.344817412286837
32	21.93587440281358	26.225920349287396	29.05169082192521	22.786514425973817
33	21.812039167395042	26.233538848110683	29.60908789946906	22.34533408502522
34	21.43277644415571	26.457938267996628	30.005942429082168	22.103342858765497
35	21.090498069749433	27.887861238047613	29.195334154284296	21.826306537918654
36	22.68456505990218	26.10513251339817	29.056538957540027	22.153763469159625
37	21.838773172356763	26.43577536232888	29.12275063822242	22.602700827091937
38	21.694021694714287	25.414480965526987	30.25347438175882	22.638022957999908
39	21.152000132977435	25.389547696650773	30.930135595427238	22.528316574944558
40	20.489052217190935	25.96619879849348	30.623733424570627	22.921015559744962
41	21.311988608266486	24.918724469371554	30.7489538415934	23.020333080768555
42	20.301498627977622	26.596179392099202	30.284086895212397	22.818235084710782
43	20.53711801885786	26.331748223850887	29.70494246648207	23.426191290809182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	84.5
2	123.0
3	366.5
4	610.0
5	610.0
6	924.5
7	1239.0
8	1362.0
9	1485.0
10	2172.5
11	2860.0
12	2860.0
13	5078.0
14	7296.0
15	11178.5
16	15061.0
17	14053.0
18	13045.0
19	13045.0
20	13987.0
21	14929.0
22	12194.5
23	9460.0
24	10756.0
25	12052.0
26	12052.0
27	13341.0
28	14630.0
29	15961.0
30	17292.0
31	19561.0
32	21830.0
33	21830.0
34	24175.5
35	26521.0
36	29227.5
37	31934.0
38	33991.0
39	36048.0
40	36048.0
41	37793.5
42	39539.0
43	42120.5
44	44702.0
45	46659.5
46	48617.0
47	48617.0
48	59498.5
49	70380.0
50	67419.0
51	64458.0
52	60279.0
53	56100.0
54	56100.0
55	50469.5
56	44839.0
57	39072.0
58	33305.0
59	29683.0
60	26061.0
61	26061.0
62	22985.5
63	19910.0
64	17566.5
65	15223.0
66	13035.5
67	10848.0
68	10848.0
69	9219.0
70	7590.0
71	6553.5
72	5517.0
73	4540.0
74	3563.0
75	3563.0
76	2843.0
77	2123.0
78	1682.0
79	1241.0
80	957.5
81	674.0
82	674.0
83	517.5
84	361.0
85	277.0
86	193.0
87	152.5
88	112.0
89	112.0
90	93.5
91	75.0
92	48.5
93	22.0
94	16.5
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	721927.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.95038016046088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6512319868362	51.34823918822657
2	8.10602332601049	9.71918359967785
3	2.2779185171103	4.096862432259474
4	1.0449447317617289	2.505793356631459
5	0.6052045304941699	1.8141120838979365
6	0.4233275715232718	1.5227189307134903
7	0.2958373170663129	1.2414891736643379
8	0.22811714613354778	1.0940567705460478
9	0.16589670362825762	0.8951013404893219
>10	1.0619323600438562	11.857831682947783
>50	0.08826857313798943	3.6235884703015095
>100	0.046444794986788045	5.190173243932299
>500	0.0025417549494359485	1.0359013403088917
>1k	0.001617480422368331	1.4628400847368142
>5k	6.932058953007132E-4	2.5921083016662183
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7148	0.9901278107066227	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6411	0.8880399264745604	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5153	0.7137840806618951	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2709	0.37524569658705104	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1407	0.1948950517157552	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1403	0.1943409790740615	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1373	0.19018543426135884	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1328	0.18395211704230482	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1259	0.1743943639730887	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1081	0.14973813141771952	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.10749009248857572	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.10596639272391806	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	756	0.10471972928010725	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	745	0.10319602951544962	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.10042566630698117	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.770363208468446E-4	0.0
10	1.385181604234223E-4	0.0	0.0	4.1555448127026696E-4	0.0
11	1.385181604234223E-4	0.0	0.0	9.696271229639562E-4	0.0
12	1.385181604234223E-4	0.0	0.0	0.0019392542459279124	0.0
13	1.385181604234223E-4	0.0	0.0	0.0019392542459279124	0.0
14	1.385181604234223E-4	0.0	0.0	0.002077772406351335	0.0
15	1.385181604234223E-4	0.0	0.0	0.002493326887621602	0.0
16	1.385181604234223E-4	0.0	0.0	0.003047399529315291	0.0
17	1.385181604234223E-4	0.0	0.0	0.004571099293972937	0.0
18	1.385181604234223E-4	0.0	0.0	0.004986653775243204	0.0
19	1.385181604234223E-4	0.0	0.0	0.006094799058630582	0.0
20	1.385181604234223E-4	0.0	0.0	0.008865162267099028	0.0
21	1.385181604234223E-4	0.0	0.0	0.015375515806999876	0.0
22	1.385181604234223E-4	0.0	0.0	0.023409569111558372	0.0
23	1.385181604234223E-4	0.0	0.0	0.030058440811882643	0.0
24	1.385181604234223E-4	0.0	0.0	0.03961619388109878	0.0
25	1.385181604234223E-4	0.0	0.0	0.04668062006269332	0.0
26	1.385181604234223E-4	0.0	0.0	0.0583161455382608	0.0
27	1.385181604234223E-4	0.0	0.0	0.1098449012157739	0.0
28	1.385181604234223E-4	0.0	0.0	0.21650388474180907	0.0
29	1.385181604234223E-4	0.0	0.0	0.36707312512206913	0.0
30	1.385181604234223E-4	0.0	0.0	0.5657081671692568	0.0
31	1.385181604234223E-4	0.0	0.0	0.867539238731894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	55	1.9013882E-5	23.545454	21
ATTAGAC	60	3.7238147E-5	21.583334	3
GGTATCA	2390	0.0	21.364017	1
GACAGTA	45	0.0038244491	20.555557	7
GACCGTC	45	0.0038244491	20.555557	22
ACCGTCG	45	0.0038244491	20.555557	23
GGCGTCG	55	5.1405473E-4	20.181818	8
GTCGTCA	65	6.8975874E-5	19.923077	24
TAGTGTC	65	6.8975874E-5	19.923077	19
GTATAGA	75	9.257945E-6	19.733334	1
TACCCGA	145	7.2759576E-12	19.13793	11
TTCGCTA	155	1.8189894E-12	19.096775	26
GGGCGTC	70	1.2184004E-4	18.5	7
TATACTG	80	1.6153132E-5	18.5	5
TCTATAC	60	9.232278E-4	18.5	3
GGCTAAC	50	0.0070324107	18.499998	1
CGAAATT	115	6.395021E-8	17.695652	13
TTACCCG	160	3.45608E-11	17.34375	10
TGTGCGC	75	2.0659246E-4	17.266666	10
TCCTACA	75	2.0659246E-4	17.266666	2
>>END_MODULE