FastQCFastQC Report
Wed 25 May 2016
SRR2088634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904251
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT170400.8948400184639524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158790.8338711650932572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126690.665300950347407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67020.3519494016282517No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36830.19340937723020757No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36730.1928842363743015No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36430.19130881380658327No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32040.16825513023230657No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA30270.15896013708276904No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG26870.14110534798196245No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19530.10256000915845652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.021.5715641
TTAGTAC701.21973135E-418.53
CTAATAC4950.017.5656573
GTATTAG4850.017.5463921
CGGGTAA2200.016.81818226
ATTAGAG4200.016.7380943
TTAACGG3550.016.67605635
TCTAATA5050.016.485152
GTTCCGA2150.016.34883733
ACGTTCG2100.015.85714222
TCGGGTA2450.015.85714225
ACAGCGT1059.3494655E-615.8571428
TAACGGC3800.015.57894836
GTACCGT1557.215931E-915.5161296
TAGCGAA2750.015.47272710
TACTGGT4800.015.4166677
ACCGTCC855.36645E-415.2352938
TAATACT5500.015.1363634
GGTAAAC4100.014.89024435
TTCGTTA2006.184564E-1114.830
Produced by FastQC (version 0.11.5)