##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1904251
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754574370710582	31.0	31.0	34.0	30.0	34.0
2	31.939542633822956	31.0	31.0	34.0	30.0	34.0
3	32.024590245718656	33.0	31.0	34.0	30.0	34.0
4	35.699803754862145	37.0	35.0	37.0	35.0	37.0
5	35.605385529533656	37.0	35.0	37.0	33.0	37.0
6	35.63762130097346	37.0	35.0	37.0	33.0	37.0
7	35.51268819078997	37.0	35.0	37.0	33.0	37.0
8	35.533629757841794	37.0	35.0	37.0	33.0	37.0
9	37.15994897731444	39.0	37.0	39.0	33.0	39.0
10	37.01757593930632	39.0	37.0	39.0	33.0	39.0
11	37.05994745440596	39.0	37.0	39.0	33.0	39.0
12	36.96121309638278	39.0	37.0	39.0	33.0	39.0
13	36.99942917188963	39.0	37.0	39.0	33.0	39.0
14	38.06273595235082	40.0	37.0	41.0	33.0	41.0
15	38.01988603393145	40.0	37.0	41.0	33.0	41.0
16	37.926537520526445	40.0	37.0	41.0	33.0	41.0
17	37.93265908748374	40.0	37.0	41.0	33.0	41.0
18	37.90672710687824	40.0	37.0	41.0	33.0	41.0
19	37.920695328504486	40.0	37.0	41.0	32.0	41.0
20	37.796964790881034	40.0	37.0	41.0	32.0	41.0
21	37.69147187004234	40.0	37.0	41.0	32.0	41.0
22	37.54339527719823	39.0	36.0	41.0	32.0	41.0
23	37.40750667847883	39.0	36.0	41.0	32.0	41.0
24	37.26543454618115	39.0	36.0	41.0	31.0	41.0
25	37.06912534114463	39.0	36.0	41.0	31.0	41.0
26	37.239371280361674	39.0	36.0	41.0	31.0	41.0
27	37.178827265943404	39.0	36.0	41.0	31.0	41.0
28	37.05858418874403	39.0	36.0	41.0	31.0	41.0
29	36.92413815195581	39.0	36.0	41.0	30.0	41.0
30	36.81293432430914	39.0	35.0	41.0	30.0	41.0
31	36.64439246716951	39.0	35.0	40.0	30.0	41.0
32	36.523314416009235	39.0	35.0	40.0	30.0	41.0
33	36.32464312740285	39.0	35.0	40.0	30.0	41.0
34	36.195425392976034	38.0	35.0	40.0	30.0	41.0
35	36.01542968862823	38.0	35.0	40.0	29.0	41.0
36	35.87928744687544	38.0	35.0	40.0	28.0	41.0
37	35.72493542080324	38.0	35.0	40.0	27.0	41.0
38	35.65351206327317	38.0	35.0	40.0	27.0	41.0
39	35.53096151715294	38.0	35.0	40.0	26.0	41.0
40	35.36604995875019	38.0	34.0	40.0	25.0	41.0
41	35.25010726001982	38.0	34.0	40.0	25.0	41.0
42	35.044112357036965	38.0	34.0	40.0	24.0	41.0
43	34.70018710768696	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	2.0
14	3.0
15	15.0
16	25.0
17	72.0
18	124.0
19	270.0
20	646.0
21	1285.0
22	2365.0
23	4097.0
24	6532.0
25	10237.0
26	14743.0
27	20686.0
28	28094.0
29	37091.0
30	47022.0
31	58332.0
32	72962.0
33	90279.0
34	112840.0
35	140618.0
36	184147.0
37	262730.0
38	371130.0
39	437900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99015781007861	20.85035008515159	13.338892824527859	23.820599280241943
2	19.01613810364285	22.424774885243597	35.10802935117272	23.451057659940837
3	19.762730858484517	24.703518601276826	31.596044849129655	23.937705691109
4	13.929728801507785	16.130541614524553	36.22395366997313	33.715775913994534
5	13.30969499293948	37.84401321044337	34.88557968461091	13.960712112006243
6	32.86751588944945	36.92727481828813	13.97000710515578	16.235202187106637
7	26.62112295070345	32.04819112606479	22.06494837077675	19.265737552455008
8	26.74783943923359	34.75224642129635	19.089132682613794	19.410781456856267
9	24.742221482357106	14.441583593759436	21.41393125170999	39.40226367217347
10	16.762338578265155	26.755769066157768	33.58664377752723	22.89524857804985
11	33.66520484957077	22.320376883089466	23.20308614778199	20.81133211955777
12	20.999647630485686	26.547130604106282	30.93252937769233	21.520692387715695
13	31.06654532411956	20.691337433983232	25.980818705097175	22.261298536800034
14	20.868362416509168	22.241605754703556	27.189115300451466	29.700916528335814
15	23.610070311109197	28.85638500386766	24.80786408934536	22.725680595677776
16	22.68540229202978	27.625559865795	26.171339807619898	23.517698034555316
17	22.23698451517158	26.5179852866035	26.73418577698003	24.510844421244887
18	23.110398786714565	25.521793082949674	28.734709867554226	22.633098262781534
19	23.393712278475896	24.808179173858907	29.467123819286428	22.33098472837877
20	23.95860629717406	24.649429093118503	29.228394786191526	22.163569823515914
21	23.075792004310355	25.45662310273173	29.46197743889855	22.005607454059366
22	22.22753197976527	25.698870579561202	29.1396459815434	22.933951459130125
23	22.766326497924904	25.827162490659056	30.0057082811037	21.40080273031234
24	22.899292162640325	25.986805310854503	27.99588919737997	23.118013329125205
25	22.971394002156227	25.743980179083536	28.82177822146345	22.462847597296783
26	22.63708933328642	26.41432248164764	28.95133047061548	21.99725771445046
27	22.099817723608915	26.409858784372435	28.52906470838141	22.96125878363724
28	22.232100705211653	26.20626167453765	28.817104467845887	22.744533152404802
29	22.58951157174133	26.16776884979974	28.348101169436173	22.894618409022762
30	22.278628185044933	26.234776823013352	29.587486103460105	21.899108888481614
31	22.765118673956323	25.877943611425174	29.56758326502126	21.78935444959724
32	22.379035116694176	25.662845916846045	28.77656359376994	23.18155537268984
33	22.239137592680798	25.85089885734601	29.254966913500375	22.65499663647282
34	21.86949094420851	25.98953604330522	29.652918654105996	22.48805435838028
35	21.652240172120166	27.464026538518294	28.74710319175361	22.13663009760793
36	23.010884594520363	25.697400185164664	28.89004653273124	22.40166868758373
37	22.060012046731234	26.014742804388707	28.858328025034513	23.066917123845542
38	21.989853228382184	25.030996439019855	29.995625576670303	22.983524755927657
39	21.49711356328551	24.89635032356554	30.643176766088082	22.963359347060866
40	20.94792125617894	25.56879318955327	30.296268716676533	23.187016837591262
41	21.875267493623475	24.496718132221016	30.364274457516366	23.26373991663914
42	20.506855451303426	26.225744400291767	30.013217795343156	23.25418235306165
43	21.051006406193302	25.801535616890842	29.360782795965445	23.78667518095041
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	218.0
2	334.0
3	895.5
4	1457.0
5	1457.0
6	2207.5
7	2958.0
8	3241.5
9	3525.0
10	5266.5
11	7008.0
12	7008.0
13	12423.5
14	17839.0
15	26815.0
16	35791.0
17	33575.5
18	31360.0
19	31360.0
20	33537.0
21	35714.0
22	29055.0
23	22396.0
24	25860.5
25	29325.0
26	29325.0
27	32922.5
28	36520.0
29	40850.5
30	45181.0
31	50472.0
32	55763.0
33	55763.0
34	62393.5
35	69024.0
36	76674.0
37	84324.0
38	90141.0
39	95958.0
40	95958.0
41	100232.0
42	104506.0
43	111721.0
44	118936.0
45	123667.0
46	128398.0
47	128398.0
48	155418.0
49	182438.0
50	175353.0
51	168268.0
52	158156.0
53	148044.0
54	148044.0
55	134995.5
56	121947.0
57	105990.5
58	90034.0
59	81013.0
60	71992.0
61	71992.0
62	64640.5
63	57289.0
64	49960.0
65	42631.0
66	36802.5
67	30974.0
68	30974.0
69	26861.5
70	22749.0
71	19457.0
72	16165.0
73	13399.5
74	10634.0
75	10634.0
76	8577.5
77	6521.0
78	5144.5
79	3768.0
80	2955.0
81	2142.0
82	2142.0
83	1625.5
84	1109.0
85	822.0
86	535.0
87	417.5
88	300.0
89	300.0
90	246.5
91	193.0
92	125.5
93	58.0
94	40.0
95	22.0
96	22.0
97	16.5
98	11.0
99	9.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1904251.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.56989926656964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1745151918888	41.86161571458332
2	10.505800789317055	10.835661768394566
3	3.222383662068478	4.985340025533336
4	1.3972556674509375	2.882253360803535
5	0.8232300656808692	2.1226945780186965
6	0.517357684213123	1.6008050195793901
7	0.35987114347831656	1.2990963032685412
8	0.2575276419396528	1.0624539642548085
9	0.21046787424728697	0.9768426366403473
>10	1.3216917076477925	12.955385260308638
>50	0.12247228136054818	4.385548814572643
>100	0.07814371775647083	7.619901443313516
>500	0.006324390361987949	2.1504737659081705
>1k	0.002550157404027399	2.511235729866053
>5k	1.0200629616109596E-4	0.35255565506645203
>10k+	3.0601888848328783E-4	2.398135959888006
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17040	0.8948400184639524	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15879	0.8338711650932572	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12669	0.665300950347407	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6702	0.3519494016282517	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3683	0.19340937723020757	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3673	0.1928842363743015	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3643	0.19130881380658327	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3204	0.16825513023230657	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3027	0.15896013708276904	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2687	0.14110534798196245	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1953	0.10256000915845652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.251408559060754E-5	0.0
5	5.251408559060754E-5	0.0	0.0	5.251408559060754E-5	0.0
6	1.0502817118121507E-4	0.0	0.0	5.251408559060754E-5	0.0
7	1.0502817118121507E-4	0.0	0.0	5.251408559060754E-5	0.0
8	1.0502817118121507E-4	0.0	0.0	5.251408559060754E-5	0.0
9	1.0502817118121507E-4	0.0	0.0	2.625704279530377E-4	0.0
10	2.1005634236243015E-4	0.0	0.0	3.675985991342528E-4	0.0
11	2.625704279530377E-4	5.251408559060754E-5	0.0	6.826831126778981E-4	0.0
12	2.625704279530377E-4	5.251408559060754E-5	0.0	0.0013653662253557961	0.0
13	3.1508451354364525E-4	5.251408559060754E-5	0.0	0.0015754225677182263	0.0
14	3.1508451354364525E-4	5.251408559060754E-5	0.0	0.0018379929956712639	0.0
15	3.675985991342528E-4	5.251408559060754E-5	0.0	0.002153077509214909	0.0
16	4.201126847248603E-4	5.251408559060754E-5	0.0	0.00299330287866463	0.0
17	4.7262677031546785E-4	5.251408559060754E-5	0.0	0.003991070504886173	0.0
18	4.7262677031546785E-4	5.251408559060754E-5	0.0	0.004883809959926501	0.0
19	4.7262677031546785E-4	5.251408559060754E-5	0.0	0.006091633928510475	0.0
20	4.7262677031546785E-4	5.251408559060754E-5	0.0	0.009925162176624825	0.0
21	4.7262677031546785E-4	5.251408559060754E-5	0.0	0.015229084821276186	0.0
22	4.7262677031546785E-4	1.0502817118121507E-4	0.0	0.02520676108349162	0.0
23	4.7262677031546785E-4	1.0502817118121507E-4	0.0	0.031455937268773916	0.0
24	4.7262677031546785E-4	1.0502817118121507E-4	0.0	0.04232635298602968	0.0
25	4.7262677031546785E-4	1.0502817118121507E-4	0.0	0.049468268626352306	0.0
26	4.7262677031546785E-4	1.0502817118121507E-4	0.0	0.06002359983006442	0.0
27	6.301690270872905E-4	1.0502817118121507E-4	0.0	0.10760136137515484	0.0
28	6.301690270872905E-4	1.0502817118121507E-4	0.0	0.2291714695174113	0.0
29	6.301690270872905E-4	1.0502817118121507E-4	0.0	0.3727974936077229	0.0
30	6.826831126778981E-4	1.0502817118121507E-4	0.0	0.5676247511488769	0.0
31	7.351971982685056E-4	1.0502817118121507E-4	0.0	0.8768276871063742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5240	0.0	21.571564	1
TTAGTAC	70	1.21973135E-4	18.5	3
CTAATAC	495	0.0	17.565657	3
GTATTAG	485	0.0	17.546392	1
CGGGTAA	220	0.0	16.818182	26
ATTAGAG	420	0.0	16.738094	3
TTAACGG	355	0.0	16.676056	35
TCTAATA	505	0.0	16.48515	2
GTTCCGA	215	0.0	16.348837	33
ACGTTCG	210	0.0	15.857142	22
TCGGGTA	245	0.0	15.857142	25
ACAGCGT	105	9.3494655E-6	15.857142	8
TAACGGC	380	0.0	15.578948	36
GTACCGT	155	7.215931E-9	15.516129	6
TAGCGAA	275	0.0	15.472727	10
TACTGGT	480	0.0	15.416667	7
ACCGTCC	85	5.36645E-4	15.235293	8
TAATACT	550	0.0	15.136363	4
GGTAAAC	410	0.0	14.890244	35
TTCGTTA	200	6.184564E-11	14.8	30
>>END_MODULE