FastQCFastQC Report
Wed 25 May 2016
SRR2088633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523584
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT292131.1575996677740863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT247200.9795592300474246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT199520.7906215921483097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110860.4392958585884203No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54090.21433802084654205No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG53650.21259446881894956No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG51160.20272754939007379No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50010.19817053840886614No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA45330.17962548502447315No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG38850.153947718799929No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31460.124663969972864No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.11376676980041085No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27590.10932863736653903No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.10829835662296164No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27200.10778321625117293No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA25870.10251293398595016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT450.003827057520.5555551
GGTATCA91700.020.39641
GTATTAG6000.016.651
GTATTGG11700.016.2863241
ATAGTAC3850.015.3766233
CTAATAC5750.014.7999993
TATTAGA5600.014.5357152
GGACCGT4800.014.2604176
CGTTTAT1304.448746E-614.2307691
ACCGTTA3150.014.0952398
ATTAGAG5650.014.0796463
CAATGCG1458.921361E-714.03448319
CGAACGT3050.013.9508212
ATCGCGT800.00630137313.87500124
GTATCAA135800.013.7728282
TGCGACG1356.5739496E-613.70370422
CGAATTA1356.5739496E-613.70370415
TAGACAG2750.013.4545465
TTACTCG1102.4581855E-413.45454519
TGGACCC16650.013.3333335
Produced by FastQC (version 0.11.5)