FastQCFastQC Report
Wed 25 May 2016
SRR2088632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3722592
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT373191.0025004083176454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT331920.8916367950073497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257500.6917223268088472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146860.39451006180639725No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG73560.1976042499419759No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69770.187423171811469No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68370.1836623513938675No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG65740.17659738160937327No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA62000.16655061849378067No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG51390.13804897232895788No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.11091733931626135No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.1026166713945552No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37710.10130038424839467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118450.020.8193341
TAATACT11000.017.3227274
CTAATAC10250.016.6048773
TCTAATA10200.015.9607842
GTATTGG16050.015.5607481
AATACTG11650.015.4034345
TCGGTAC855.368158E-415.2352943
GCTAGAG9000.015.00555617
ATACTGG9950.014.8743726
CTCTAAT9400.014.7606391
TTGGACC23350.014.4989294
TGCTAGA9400.014.17021416
TACTGGT10450.014.162687
GTATCAA176000.014.0536942
ATTGGAC19500.014.0410273
GTATTAG10000.013.8751
CGTATAC1907.1413524E-913.6315793
TGGACCC22050.013.5918375
GGTAAAC9000.013.56666735
GATGCTA10350.013.22705314
Produced by FastQC (version 0.11.5)