##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088632_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3722592
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63610382228297	31.0	31.0	34.0	30.0	34.0
2	31.822368124145758	31.0	31.0	34.0	30.0	34.0
3	31.900846238319968	31.0	31.0	34.0	30.0	34.0
4	35.59482022203884	37.0	35.0	37.0	33.0	37.0
5	35.49588405068297	37.0	35.0	37.0	33.0	37.0
6	35.534057452441736	37.0	35.0	37.0	33.0	37.0
7	35.40837862435636	37.0	35.0	37.0	33.0	37.0
8	35.43054919797818	37.0	35.0	37.0	33.0	37.0
9	37.03252330634139	39.0	37.0	39.0	33.0	39.0
10	36.881941131340746	39.0	37.0	39.0	33.0	39.0
11	36.930031279280676	39.0	37.0	39.0	33.0	39.0
12	36.83074857518632	39.0	37.0	39.0	32.0	39.0
13	36.87893704171717	39.0	37.0	39.0	33.0	39.0
14	37.910317864541696	40.0	37.0	41.0	33.0	41.0
15	37.86885374491752	40.0	37.0	41.0	33.0	41.0
16	37.764297564707604	40.0	37.0	41.0	32.0	41.0
17	37.77882453946068	40.0	37.0	41.0	32.0	41.0
18	37.748176807987555	40.0	37.0	41.0	32.0	41.0
19	37.772387358055894	40.0	37.0	41.0	32.0	41.0
20	37.65077021602153	39.0	37.0	41.0	32.0	41.0
21	37.54485691690091	39.0	37.0	41.0	32.0	41.0
22	37.3908537384704	39.0	36.0	41.0	32.0	41.0
23	37.2543794753763	39.0	36.0	41.0	31.0	41.0
24	37.12287030112352	39.0	36.0	41.0	31.0	41.0
25	36.91406176126742	39.0	36.0	40.0	30.0	41.0
26	37.070065158900036	39.0	36.0	41.0	31.0	41.0
27	37.00276581478712	39.0	36.0	41.0	31.0	41.0
28	36.88403107297281	39.0	36.0	41.0	30.0	41.0
29	36.7432033916153	39.0	35.0	40.0	30.0	41.0
30	36.62666711796512	39.0	35.0	40.0	30.0	41.0
31	36.45120631001195	39.0	35.0	40.0	30.0	41.0
32	36.32336044347594	38.0	35.0	40.0	30.0	41.0
33	36.128084410002494	38.0	35.0	40.0	30.0	41.0
34	35.99575000429808	38.0	35.0	40.0	29.0	41.0
35	35.80834429343855	38.0	35.0	40.0	28.0	41.0
36	35.66687512356982	38.0	35.0	40.0	27.0	41.0
37	35.510705981208794	38.0	34.0	40.0	27.0	41.0
38	35.426107669065	38.0	34.0	40.0	26.0	41.0
39	35.29424390317284	38.0	34.0	40.0	25.0	41.0
40	35.117054192347695	38.0	34.0	40.0	24.0	41.0
41	34.98909926201958	38.0	34.0	40.0	24.0	41.0
42	34.78186247646801	38.0	33.0	40.0	23.0	41.0
43	34.42864353654658	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	5.0
13	4.0
14	16.0
15	28.0
16	66.0
17	125.0
18	338.0
19	702.0
20	1429.0
21	2925.0
22	5313.0
23	8892.0
24	14537.0
25	21737.0
26	31562.0
27	44104.0
28	59963.0
29	77845.0
30	98357.0
31	121315.0
32	149341.0
33	184762.0
34	227734.0
35	283927.0
36	369920.0
37	521046.0
38	732775.0
39	763819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54607864627658	21.24946811254094	13.705907066903922	23.498546174278566
2	19.37297990217569	22.96848539942062	34.54611732900087	23.112417369402824
3	20.196787614651296	24.6007889126716	31.689451865796737	23.512971606880367
4	14.344118291770897	16.4069551538283	35.82205624468113	33.42687030971968
5	13.359481780436857	38.076936715063056	34.62060843627236	13.94297306822773
6	32.91910582733751	36.74305430194875	13.901953262672889	16.435886608040846
7	26.653793915637277	32.17142786531535	21.794384128048414	19.38039409099896
8	26.815240563564313	34.48798579054594	19.110017965976393	19.586755679913352
9	24.77061681752929	14.456351918233318	21.541307776946816	39.23172348729057
10	16.831068244921816	27.040459980572678	33.516377835658595	22.61209393884691
11	33.669362637646024	22.29650737980418	23.135922497012835	20.89820748553696
12	20.98543165622233	26.7495605212712	30.72539241474757	21.539615407758895
13	31.107867851217648	20.626434484359287	26.090691647110397	22.17500601731267
14	20.79217921276358	22.464025066405345	27.330661001796596	29.413134719034478
15	23.569437639150355	29.00108311628027	24.941008845449623	22.488470399119752
16	22.455590083468724	27.676656480215932	26.36259896330299	23.50515447301235
17	22.1490832194342	26.53833672881691	27.009540664139397	24.303039387609495
18	22.98594635135948	25.489712544377678	29.000196637181837	22.524144467081
19	23.213126767585596	24.87841804849954	29.773072096001922	22.13538308791294
20	23.84198966741453	24.67036409039723	29.63212191935082	21.85552432283742
21	22.95943256741539	25.415678108156897	29.840766863518752	21.784122460908957
22	22.04885735530512	25.72446832744496	29.4668875880032	22.759786729246716
23	22.570563736235396	25.881831798918604	30.334804351376675	21.212800113469324
24	22.703723642021473	26.11188655646388	28.17539499359586	23.008994807918786
25	22.852571541549544	25.89330230119229	29.085513534655423	22.168612622602744
26	22.450808468937772	26.53336707326508	29.221735822781547	21.7940886350156
27	21.825222855472745	26.502420893828816	28.805198098529196	22.867158152169242
28	21.970444249598128	26.22876748244234	29.277772047003808	22.52301622095572
29	22.328716120380637	26.259310716833863	28.720982584177907	22.690990578607597
30	22.17763859160499	26.293158100592272	29.939300358459914	21.589902949342825
31	22.662462069439787	25.935826434914166	29.758592937394155	21.643118558251885
32	22.070535798712296	25.913422690426458	29.06055780488434	22.955483705976913
33	21.975091549114165	25.931770121463753	29.565931480001034	22.52720684942105
34	21.58976863432791	26.160374276847957	29.95208714787976	22.297769940944374
35	21.43493028513466	27.62687933568868	28.99450168054947	21.94368869862719
36	22.900629453885895	25.834526050665772	29.014326576750822	22.25051791869751
37	21.909868177871765	26.07916741883075	29.142006429928397	22.868957973369096
38	21.853214104580896	25.0489175284318	30.327981148619028	22.769887218368275
39	21.233968159819824	25.054827389088036	30.905267082720854	22.805937368371286
40	20.68435111879035	25.70499265028238	30.584684005123314	23.025972225803955
41	21.489086099148118	24.678584169309985	30.733263274621553	23.09906645692034
42	20.42359194883565	26.32907393558037	30.17891834506709	23.068415770516886
43	20.732973154189338	25.93426838074116	29.68600910333445	23.64674936173505
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	434.5
2	670.0
3	1745.0
4	2820.0
5	2820.0
6	4576.5
7	6333.0
8	7003.5
9	7674.0
10	11107.0
11	14540.0
12	14540.0
13	26438.0
14	38336.0
15	58413.5
16	78491.0
17	72042.5
18	65594.0
19	65594.0
20	70884.5
21	76175.0
22	61330.0
23	46485.0
24	52258.0
25	58031.0
26	58031.0
27	63865.5
28	69700.0
29	77602.5
30	85505.0
31	95497.0
32	105489.0
33	105489.0
34	119071.0
35	132653.0
36	146386.5
37	160120.0
38	173149.5
39	186179.0
40	186179.0
41	194714.0
42	203249.0
43	219275.0
44	235301.0
45	245888.0
46	256475.0
47	256475.0
48	309253.0
49	362031.0
50	345694.5
51	329358.0
52	308609.5
53	287861.0
54	287861.0
55	260739.0
56	233617.0
57	203791.0
58	173965.0
59	155988.5
60	138012.0
61	138012.0
62	123311.0
63	108610.0
64	94682.5
65	80755.0
66	69483.5
67	58212.0
68	58212.0
69	50532.0
70	42852.0
71	36375.0
72	29898.0
73	24735.5
74	19573.0
75	19573.0
76	15705.5
77	11838.0
78	9450.0
79	7062.0
80	5626.5
81	4191.0
82	4191.0
83	3154.5
84	2118.0
85	1638.5
86	1159.0
87	925.0
88	691.0
89	691.0
90	591.5
91	492.0
92	330.0
93	168.0
94	121.5
95	75.0
96	75.0
97	49.5
98	24.0
99	17.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3722592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.52238752670709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34049619234872	34.43383542983564
2	12.014818935465707	10.698568494160453
3	4.117205813643238	5.499234982867069
4	1.8729009452538785	3.3354408673491687
5	1.0369648527522568	2.3084075512905367
6	0.6703934359047958	1.7908509809228392
7	0.4615746872418621	1.4385284968530567
8	0.3330925661477619	1.1864061049836778
9	0.2539434418472568	1.0175551495010438
>10	1.6163699147707082	13.744877114009322
>50	0.16194750499311966	5.021090648733948
>100	0.10575955245032308	9.067991274464053
>500	0.009385351514654205	2.907484711783782
>1k	0.004541299116965227	3.5051298521243273
>5k	3.633039293572181E-4	1.0536228552025095
>10k+	2.4220261957147874E-4	2.990975485918502
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37319	1.0025004083176454	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33192	0.8916367950073497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25750	0.6917223268088472	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14686	0.39451006180639725	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7356	0.1976042499419759	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6977	0.187423171811469	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6837	0.1836623513938675	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6574	0.17659738160937327	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6200	0.16655061849378067	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5139	0.13804897232895788	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4129	0.11091733931626135	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3820	0.1026166713945552	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3771	0.10130038424839467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.6863002982867852E-5	0.0
4	0.0	0.0	0.0	2.6863002982867852E-5	0.0
5	2.6863002982867852E-5	0.0	0.0	2.6863002982867852E-5	0.0
6	5.3726005965735705E-5	0.0	0.0	2.6863002982867852E-5	0.0
7	5.3726005965735705E-5	0.0	0.0	2.6863002982867852E-5	0.0
8	5.3726005965735705E-5	0.0	0.0	2.6863002982867852E-5	0.0
9	8.058900894860356E-5	0.0	0.0	1.6117801789720712E-4	0.0
10	1.6117801789720712E-4	0.0	0.0	2.9549303281154637E-4	0.0
11	1.6117801789720712E-4	0.0	0.0	5.641230626402249E-4	0.0
12	1.6117801789720712E-4	0.0	0.0	9.402051044003748E-4	0.0
13	1.6117801789720712E-4	0.0	0.0	0.0010476571163318463	0.0
14	1.8804102088007497E-4	0.0	0.0	0.0011819721312461855	0.0
15	2.1490402386294282E-4	0.0	0.0	0.0017460951938864104	0.0
16	2.1490402386294282E-4	0.0	0.0	0.002310218256526635	0.0
17	2.1490402386294282E-4	0.0	0.0	0.0033310123698756136	0.0
18	2.1490402386294282E-4	0.0	0.0	0.003975724441464442	0.0
19	2.686300298286785E-4	0.0	0.0	0.004942792548847685	0.0
20	2.686300298286785E-4	0.0	0.0	0.008488708942586241	0.0
21	2.9549303281154637E-4	0.0	0.0	0.014183665574954225	0.0
22	3.2235603579441424E-4	0.0	0.0	0.023075319562283484	0.0
23	3.2235603579441424E-4	0.0	0.0	0.0294955772751889	0.0
24	3.2235603579441424E-4	0.0	0.0	0.03943488837885001	0.0
25	3.2235603579441424E-4	0.0	0.0	0.0467953511961558	0.0
26	3.4921903877728206E-4	0.0	0.0	0.05853448349966905	0.0
27	3.4921903877728206E-4	0.0	0.0	0.10925183313132356	0.0
28	3.4921903877728206E-4	0.0	0.0	0.22454784193379237	0.0
29	3.4921903877728206E-4	0.0	0.0	0.3718645502918397	0.0
30	3.4921903877728206E-4	0.0	0.0	0.5751637568661836	0.0
31	3.4921903877728206E-4	0.0	0.0	0.8914756169894525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11845	0.0	20.819334	1
TAATACT	1100	0.0	17.322727	4
CTAATAC	1025	0.0	16.604877	3
TCTAATA	1020	0.0	15.960784	2
GTATTGG	1605	0.0	15.560748	1
AATACTG	1165	0.0	15.403434	5
TCGGTAC	85	5.368158E-4	15.235294	3
GCTAGAG	900	0.0	15.005556	17
ATACTGG	995	0.0	14.874372	6
CTCTAAT	940	0.0	14.760639	1
TTGGACC	2335	0.0	14.498929	4
TGCTAGA	940	0.0	14.170214	16
TACTGGT	1045	0.0	14.16268	7
GTATCAA	17600	0.0	14.053694	2
ATTGGAC	1950	0.0	14.041027	3
GTATTAG	1000	0.0	13.875	1
CGTATAC	190	7.1413524E-9	13.631579	3
TGGACCC	2205	0.0	13.591837	5
GGTAAAC	900	0.0	13.566667	35
GATGCTA	1035	0.0	13.227053	14
>>END_MODULE