FastQCFastQC Report
Wed 25 May 2016
SRR2088631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466964
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT350231.0101922027456876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT299010.8624548740627246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT242620.699805362847725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133720.38569768823673967No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64810.18693588972945782No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60130.17343704751477085No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59270.17095649103942237No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58230.1679567483250475No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA52280.15079475875722967No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG46710.1347288290273565No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36170.1043275903643649No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34870.1005779119713963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112300.020.707481
GTATTAG8000.017.806251
GTTATCG1950.017.07692311
GTATTGG15300.015.5980391
TTAACGG4500.015.21111135
ATTAGAG6100.014.5573763
TATACAG4350.014.459775
GGACCGT8450.014.230776
TAAACGT2009.858923E-1013.8749994
GTATCAA168400.013.8090852
CGAATTA2304.0017767E-1113.67391315
ACCGTTA6600.013.4545458
TACCCCG4000.013.4124995
GACCGTT7200.013.3611117
ATTGGAC17500.013.3200013
CGGAATA850.0094097313.05882428
TCTAACG1703.737532E-713.0588242
TCTATAC3700.013.03
TTGGACC20700.012.8695654
TGGACCC19950.012.7969925
Produced by FastQC (version 0.11.5)