##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3466964
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752925037583314	31.0	31.0	34.0	30.0	34.0
2	31.936356420199346	31.0	31.0	34.0	30.0	34.0
3	32.01933074586295	33.0	31.0	34.0	30.0	34.0
4	35.69418776774146	37.0	35.0	37.0	35.0	37.0
5	35.60306019906754	37.0	35.0	37.0	33.0	37.0
6	35.6311314452645	37.0	35.0	37.0	33.0	37.0
7	35.50698247804131	37.0	35.0	37.0	33.0	37.0
8	35.5244799196069	37.0	35.0	37.0	33.0	37.0
9	37.14509524759992	39.0	37.0	39.0	33.0	39.0
10	37.00566028375258	39.0	37.0	39.0	33.0	39.0
11	37.05454368721452	39.0	37.0	39.0	33.0	39.0
12	36.96233563428983	39.0	37.0	39.0	33.0	39.0
13	37.00500207097622	39.0	37.0	39.0	33.0	39.0
14	38.06167644082834	40.0	37.0	41.0	33.0	41.0
15	38.02417388816267	40.0	37.0	41.0	33.0	41.0
16	37.93109908265561	40.0	37.0	41.0	33.0	41.0
17	37.94456648525915	40.0	37.0	41.0	33.0	41.0
18	37.91777503314139	40.0	37.0	41.0	33.0	41.0
19	37.94071268118157	40.0	37.0	41.0	33.0	41.0
20	37.81770794274183	40.0	37.0	41.0	32.0	41.0
21	37.714520254608935	40.0	37.0	41.0	32.0	41.0
22	37.55976641234233	39.0	36.0	41.0	32.0	41.0
23	37.44286240064795	39.0	36.0	41.0	32.0	41.0
24	37.303693952403314	39.0	36.0	41.0	31.0	41.0
25	37.10817101071716	39.0	36.0	41.0	31.0	41.0
26	37.2657330736633	39.0	36.0	41.0	31.0	41.0
27	37.20134475004644	39.0	36.0	41.0	31.0	41.0
28	37.087466440378385	39.0	36.0	41.0	31.0	41.0
29	36.94162558365186	39.0	36.0	41.0	31.0	41.0
30	36.82142791214446	39.0	35.0	41.0	30.0	41.0
31	36.653075428530556	39.0	35.0	40.0	30.0	41.0
32	36.53191005156096	39.0	35.0	40.0	30.0	41.0
33	36.336501907720994	39.0	35.0	40.0	30.0	41.0
34	36.205139424580125	38.0	35.0	40.0	30.0	41.0
35	36.02745687581411	38.0	35.0	40.0	29.0	41.0
36	35.89271102901559	38.0	35.0	40.0	28.0	41.0
37	35.72915813374468	38.0	35.0	40.0	27.0	41.0
38	35.64690778444772	38.0	35.0	40.0	27.0	41.0
39	35.5199967464329	38.0	35.0	40.0	26.0	41.0
40	35.357391942921815	38.0	34.0	40.0	25.0	41.0
41	35.23019535247554	38.0	34.0	40.0	25.0	41.0
42	35.0253821499156	38.0	34.0	40.0	24.0	41.0
43	34.66816528813106	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	3.0
14	6.0
15	21.0
16	47.0
17	99.0
18	214.0
19	533.0
20	1103.0
21	2314.0
22	4259.0
23	7293.0
24	11672.0
25	18234.0
26	26537.0
27	38005.0
28	51610.0
29	67681.0
30	85380.0
31	106728.0
32	131894.0
33	164882.0
34	205310.0
35	256663.0
36	335719.0
37	476378.0
38	666772.0
39	807598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.008310441066016	20.8477503660263	13.455057508529075	23.68888168437861
2	18.9834679564022	22.81289912442125	34.95386741829451	23.249765500882038
3	19.87586257024878	24.590419744768045	31.939010615627968	23.594707069355206
4	13.86083039800817	16.215541897752615	36.34136379841267	33.58226390582654
5	13.3254051671722	37.99078963611967	34.808379896647324	13.875425300060803
6	32.70094526507919	37.09755855555466	14.06752420850058	16.133971970865577
7	26.492631593521015	32.30950768453321	21.94597347996691	19.25188724197886
8	26.777318714587174	34.6056953576674	19.23212932121591	19.384856606529517
9	24.880616008703868	14.433117851815016	21.524682690676915	39.1615834488042
10	16.66152287707631	27.11879904146683	33.56660178761591	22.65307629384095
11	33.562044486184455	22.501704661484805	23.248957877843555	20.687292974487185
12	20.878959227727776	26.788510062406186	30.990832324765993	21.341698385100045
13	30.952672136197553	20.79462030756593	26.12680720076701	22.12590035546951
14	20.72438594689763	22.59083163251767	27.507381097698158	29.17740132288654
15	23.519252002616696	29.041374528261617	24.969973729176306	22.46939973994538
16	22.331382731404194	27.878253134442698	26.36283503376441	23.427529100388696
17	22.081221495233294	26.690787674749433	27.10847300404619	24.11951782597108
18	22.9025164380132	25.576037132199815	28.983023763731037	22.53842266605595
19	23.105547101152478	25.14107443861546	29.84213277092003	21.911245689312032
20	23.76987473766673	24.844446034051696	29.484903794789908	21.900775433491663
21	22.736636434644257	25.641252692557526	29.85626617409353	21.76584469870469
22	22.01392918991948	25.931968142732376	29.516689530090307	22.537413137257843
23	22.484138860397742	26.00782702099012	30.199102153930646	21.30893196468149
24	22.573900392389422	26.21529961084107	28.375143208870927	22.835656787898575
25	22.64370209785853	26.000818006763264	29.224329990158537	22.13114990521967
26	22.339747398588507	26.693181700184947	29.20298566699856	21.764085234227988
27	21.73218412420781	26.71916985581621	28.873590842016245	22.675055177959734
28	21.95304018155366	26.424675883568447	29.14593286806555	22.47635106681235
29	22.220940280891295	26.48726666905108	28.811807679572098	22.47998537048553
30	22.0843654563474	26.51230298324413	29.932644238590306	21.470687321818165
31	22.387224095779477	26.24809487493957	29.828374335585835	21.536306693695117
32	22.07490472932514	26.096550180503748	29.07442938547963	22.754115704691483
33	21.882027041526822	26.13447961963262	29.541783531643244	22.441709807197306
34	21.500280937442675	26.36121978768744	29.908675140555253	22.229824134314633
35	21.294423593668697	27.78272286646184	29.097042830557225	21.82581070931224
36	22.7204551301946	26.09929033009861	29.114118289085205	22.066136250621582
37	21.857942568771985	26.321069385202733	29.168661687862922	22.65232635816236
38	21.8095140301428	25.294378597527984	30.319870641864178	22.57623673046504
39	21.149224508820975	25.30750247190337	30.912579421072735	22.63069359820292
40	20.634105228666925	25.835168752833894	30.62405609057377	22.906669927925414
41	21.367542322331584	24.89769146723185	30.74831466378076	22.986451546655807
42	20.378204100186792	26.481122965222596	30.21231255934587	22.92836037524474
43	20.667131242204995	26.04777551771521	29.80143433851635	23.48365890156344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	423.0
2	670.0
3	1797.0
4	2924.0
5	2924.0
6	4488.5
7	6053.0
8	6496.0
9	6939.0
10	10151.0
11	13363.0
12	13363.0
13	24082.5
14	34802.0
15	53324.5
16	71847.0
17	66425.5
18	61004.0
19	61004.0
20	65986.0
21	70968.0
22	58095.0
23	45222.0
24	51489.0
25	57756.0
26	57756.0
27	64110.5
28	70465.0
29	77867.0
30	85269.0
31	94384.5
32	103500.0
33	103500.0
34	115701.5
35	127903.0
36	141774.5
37	155646.0
38	166083.5
39	176521.0
40	176521.0
41	182947.0
42	189373.0
43	203146.0
44	216919.0
45	225663.0
46	234407.0
47	234407.0
48	281454.0
49	328501.0
50	315427.5
51	302354.0
52	283329.5
53	264305.0
54	264305.0
55	239532.0
56	214759.0
57	187958.0
58	161157.0
59	144154.0
60	127151.0
61	127151.0
62	112809.5
63	98468.0
64	86521.0
65	74574.0
66	64195.0
67	53816.0
68	53816.0
69	46722.5
70	39629.0
71	33591.0
72	27553.0
73	22912.5
74	18272.0
75	18272.0
76	14619.0
77	10966.0
78	8541.0
79	6116.0
80	4838.0
81	3560.0
82	3560.0
83	2726.5
84	1893.0
85	1448.5
86	1004.0
87	761.0
88	518.0
89	518.0
90	462.5
91	407.0
92	271.5
93	136.0
94	97.0
95	58.0
96	58.0
97	44.0
98	30.0
99	20.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3466964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.27176318426919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55247938874894	35.88489960629108
2	12.202066467563775	11.292222598916462
3	4.105775208600353	5.69944374420597
4	1.8588512554529737	3.440493003484059
5	1.0171841223796811	2.3533451412775652
6	0.6118404157889471	1.6986560895570575
7	0.4330566659578306	1.4026806844798936
8	0.2986519543127887	1.1055322003584434
9	0.23139051934334265	0.9636162582726191
>10	1.4265350709827742	12.553930326580872
>50	0.14658963051796645	4.748065658044931
>100	0.10279101034339574	9.31765475463565
>500	0.008359174932094962	2.6736108575040305
>1k	0.003867677952825558	3.0547759046553153
>5k	3.119095123246418E-4	0.8507153564902007
>10k+	2.495276098597134E-4	2.960357815245725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35023	1.0101922027456876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29901	0.8624548740627246	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24262	0.699805362847725	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13372	0.38569768823673967	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6481	0.18693588972945782	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6013	0.17343704751477085	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5927	0.17095649103942237	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5823	0.1679567483250475	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5228	0.15079475875722967	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4671	0.1347288290273565	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3617	0.1043275903643649	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3487	0.1005779119713963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.884367994591233E-5	0.0	0.0	0.0	0.0
3	2.884367994591233E-5	0.0	0.0	0.0	0.0
4	2.884367994591233E-5	0.0	0.0	0.0	0.0
5	2.884367994591233E-5	0.0	0.0	0.0	2.884367994591233E-5
6	2.884367994591233E-5	0.0	0.0	0.0	2.884367994591233E-5
7	2.884367994591233E-5	0.0	0.0	0.0	8.653103983773699E-5
8	5.768735989182466E-5	0.0	0.0	2.884367994591233E-5	8.653103983773699E-5
9	5.768735989182466E-5	0.0	0.0	4.0381151924277265E-4	8.653103983773699E-5
10	1.1537471978364932E-4	0.0	0.0	4.3265519918868497E-4	8.653103983773699E-5
11	2.0190575962138633E-4	0.0	0.0	5.768735989182466E-4	8.653103983773699E-5
12	2.0190575962138633E-4	0.0	0.0	8.364667184314576E-4	8.653103983773699E-5
13	2.0190575962138633E-4	0.0	0.0	8.941540783232823E-4	8.653103983773699E-5
14	2.884367994591233E-4	0.0	0.0	9.806851181610192E-4	8.653103983773699E-5
15	2.884367994591233E-4	0.0	0.0	0.001211434557728318	1.1537471978364932E-4
16	2.884367994591233E-4	0.0	0.0	0.0015575587170792659	1.1537471978364932E-4
17	3.1728047940503566E-4	0.0	0.0	0.0023651817555648113	1.1537471978364932E-4
18	3.1728047940503566E-4	0.0	0.0	0.0027113059149157593	1.1537471978364932E-4
19	3.1728047940503566E-4	0.0	0.0	0.003720834713022691	1.1537471978364932E-4
20	3.1728047940503566E-4	0.0	0.0	0.006287922228208888	1.1537471978364932E-4
21	3.4612415935094797E-4	0.0	2.884367994591233E-5	0.01113366045912216	1.1537471978364932E-4
22	3.749678392968603E-4	0.0	2.884367994591233E-5	0.01952717132338265	1.1537471978364932E-4
23	3.749678392968603E-4	0.0	2.884367994591233E-5	0.02486325211337643	1.1537471978364932E-4
24	3.749678392968603E-4	0.0	2.884367994591233E-5	0.032218390499584075	1.1537471978364932E-4
25	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.037785220729145155	1.1537471978364932E-4
26	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.04727479143135031	1.1537471978364932E-4
27	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.09832810493561514	1.1537471978364932E-4
28	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.21136648664364557	1.1537471978364932E-4
29	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.35422923341574936	1.1537471978364932E-4
30	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.5483760431316852	1.1537471978364932E-4
31	4.0381151924277265E-4	0.0	2.884367994591233E-5	0.8460428201735005	1.1537471978364932E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11230	0.0	20.70748	1
GTATTAG	800	0.0	17.80625	1
GTTATCG	195	0.0	17.076923	11
GTATTGG	1530	0.0	15.598039	1
TTAACGG	450	0.0	15.211111	35
ATTAGAG	610	0.0	14.557376	3
TATACAG	435	0.0	14.45977	5
GGACCGT	845	0.0	14.23077	6
TAAACGT	200	9.858923E-10	13.874999	4
GTATCAA	16840	0.0	13.809085	2
CGAATTA	230	4.0017767E-11	13.673913	15
ACCGTTA	660	0.0	13.454545	8
TACCCCG	400	0.0	13.412499	5
GACCGTT	720	0.0	13.361111	7
ATTGGAC	1750	0.0	13.320001	3
CGGAATA	85	0.00940973	13.058824	28
TCTAACG	170	3.737532E-7	13.058824	2
TCTATAC	370	0.0	13.0	3
TTGGACC	2070	0.0	12.869565	4
TGGACCC	1995	0.0	12.796992	5
>>END_MODULE