FastQCFastQC Report
Wed 25 May 2016
SRR2088630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148517
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT326271.0362656450640095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282030.8957550491231269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT231900.7365372332434603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128720.4088273939762752No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60940.1935514402494889No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59280.1882791168032442No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG56330.17890962634154428No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56320.17887786535692834No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA52790.1676662377875044No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG46440.14749801255638767No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.10954363594034906No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.10760621587877722No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA33400.10608168861721248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.10157162880175016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114650.018.8953341
TAATACT8950.017.1564254
GTATTGG14700.016.9897961
CTCTAAT7300.016.9794521
CTAATAC8750.016.2799993
AATACTG8900.016.2134825
TGCGACG1557.223207E-915.516129522
TCTAATA8250.014.82
TACTGGT8650.014.7572257
ATACTGG8850.014.632776
ATTGGAC17100.014.6052633
GCTAGAG7350.014.59863917
GACCGTG3300.014.5757597
TCTAGTA3050.014.5573772
AAGACCG2101.364242E-1014.0952395
TCTACGC1051.6570745E-414.0952393
TGCTAGA7750.014.08387216
GGTAAAC7500.014.05999935
TATACCG1753.5723133E-813.7428575
TTAACGG4750.013.6315835
Produced by FastQC (version 0.11.5)