##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148517
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.781291319055924	31.0	31.0	34.0	30.0	34.0
2	31.96058366526209	31.0	31.0	34.0	30.0	34.0
3	32.04748013112205	33.0	31.0	34.0	30.0	34.0
4	35.71065171317163	37.0	35.0	37.0	35.0	37.0
5	35.62568155102863	37.0	35.0	37.0	33.0	37.0
6	35.66387985200652	37.0	35.0	37.0	33.0	37.0
7	35.54172901083272	37.0	35.0	37.0	33.0	37.0
8	35.5641160584491	37.0	35.0	37.0	33.0	37.0
9	37.19454524145812	39.0	37.0	39.0	33.0	39.0
10	37.06790752598763	39.0	37.0	39.0	33.0	39.0
11	37.110870292267755	39.0	37.0	39.0	33.0	39.0
12	37.02198177745269	39.0	37.0	39.0	33.0	39.0
13	37.067924676919326	39.0	37.0	39.0	33.0	39.0
14	38.14016027228057	40.0	37.0	41.0	33.0	41.0
15	38.098837008026315	40.0	37.0	41.0	33.0	41.0
16	38.006765089723196	40.0	37.0	41.0	33.0	41.0
17	38.02248804754746	40.0	37.0	41.0	33.0	41.0
18	37.99923519549045	40.0	37.0	41.0	33.0	41.0
19	38.019275423953566	40.0	37.0	41.0	33.0	41.0
20	37.90460651792574	40.0	37.0	41.0	32.0	41.0
21	37.81098212269459	40.0	37.0	41.0	32.0	41.0
22	37.65829912939965	39.0	37.0	41.0	32.0	41.0
23	37.53558929489661	39.0	36.0	41.0	32.0	41.0
24	37.40485091870236	39.0	36.0	41.0	32.0	41.0
25	37.217919738086216	39.0	36.0	41.0	31.0	41.0
26	37.38052263970625	39.0	36.0	41.0	32.0	41.0
27	37.31481773800173	39.0	36.0	41.0	31.0	41.0
28	37.206780843171565	39.0	36.0	41.0	31.0	41.0
29	37.06463042759496	39.0	36.0	41.0	31.0	41.0
30	36.9443169593812	39.0	36.0	41.0	31.0	41.0
31	36.77332693455363	39.0	35.0	41.0	30.0	41.0
32	36.651280269409376	39.0	35.0	40.0	30.0	41.0
33	36.45642821683987	39.0	35.0	40.0	30.0	41.0
34	36.32293997459757	39.0	35.0	40.0	30.0	41.0
35	36.128198450254516	38.0	35.0	40.0	30.0	41.0
36	35.97653974871344	38.0	35.0	40.0	29.0	41.0
37	35.81984439023197	38.0	35.0	40.0	28.0	41.0
38	35.73886753668473	38.0	35.0	40.0	27.0	41.0
39	35.6132671349718	38.0	35.0	40.0	26.0	41.0
40	35.434681788283186	38.0	35.0	40.0	25.0	41.0
41	35.31163719300229	38.0	34.0	40.0	25.0	41.0
42	35.107161562094156	38.0	34.0	40.0	24.0	41.0
43	34.751696115980955	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	3.0
14	3.0
15	17.0
16	32.0
17	87.0
18	207.0
19	451.0
20	905.0
21	1919.0
22	3461.0
23	6101.0
24	10184.0
25	15618.0
26	23127.0
27	32846.0
28	44976.0
29	59084.0
30	74946.0
31	92994.0
32	115788.0
33	145419.0
34	182735.0
35	229729.0
36	302590.0
37	436372.0
38	613437.0
39	755481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88622770656789	20.9669504722382	13.699370211436051	23.447451609757863
2	18.812698168693387	22.8602227651939	35.325488158393306	23.001590907719415
3	19.91064364588154	24.753018643380358	31.781406929039925	23.55493078169818
4	14.041118405903477	16.234087349695113	35.99637543643563	33.72841880796578
5	13.38271319481521	37.8957775994222	34.66400213179729	14.057507073965297
6	32.68561040007089	36.64966712900073	14.149486885413037	16.515235585515338
7	26.326299016330545	32.32868045495705	22.05546293699542	19.28955759171699
8	26.56714256267316	34.6088015405348	19.428893031227084	19.395162865564963
9	24.59821560436231	14.698284938591724	21.952271497978256	38.75122795906771
10	16.782790119919948	27.072682154804944	33.67823645227261	22.466291273002494
11	33.06248624352354	22.564655042358037	23.455900031665703	20.916958682452723
12	20.918356165775823	26.83069521301616	31.071421878935386	21.179526742272632
13	30.895815394993896	20.761075769957728	26.3132452516534	22.029863583394977
14	20.650642826448134	22.636688955466973	27.674235203430698	29.038433014654203
15	23.22674452766175	29.08401002757806	25.2891758246819	22.400069620078277
16	22.19346441515164	27.91599981832717	26.598046000704457	23.292489765816732
17	21.947952004070487	26.638191885258994	27.28363861462396	24.13021749604655
18	22.79597029331587	25.66897367871922	29.24570520025777	22.28935082770714
19	22.941657929749148	25.05023793741625	30.151496720519532	21.856607412315068
20	23.65180178477677	24.765468949349806	29.884545644822623	21.6981836210508
21	22.573961010850503	25.637466781980216	30.204251715966596	21.584320491202682
22	21.735153407143745	26.02393444278687	29.70010960715791	22.54080254291147
23	22.29589359053802	26.1264589011271	30.51134867621804	21.066298832116832
24	22.4310048190942	26.247245925621492	28.55455441403048	22.767194841253836
25	22.514440925680248	26.139258577927322	29.333397278782357	22.01290321761007
26	22.153858467335574	26.78330782396919	29.542511601493654	21.52032210720158
27	21.47893754424702	26.80677919160036	29.18208794807206	22.532195316080557
28	21.689481111266034	26.538970569318828	29.520310673247117	22.251237646168022
29	22.053684321856927	26.552246660888283	28.96817771668376	22.42589130057103
30	21.848095468437997	26.626249755043407	30.200186309935756	21.32546846658284
31	22.28217284518394	26.25756824562167	30.06682193553346	21.393436973660933
32	21.847682575637993	26.129952609434852	29.32660042807455	22.695764386852606
33	21.61477927544936	26.196460111220617	29.84287523300652	22.345885380323498
34	21.2897055979053	26.43917755565557	30.224324658243866	22.04679218819527
35	21.056675253778206	27.82309893832557	29.31122811152044	21.808997696375787
36	22.558239323465617	26.066907054972226	29.389550699583328	21.985302921978825
37	21.58339942264882	26.313531100514943	29.479497808015648	22.623571668820592
38	21.48868816652411	25.36136854271392	30.63051589049702	22.51942740026495
39	20.963234437038135	25.24363057274266	31.207549458999267	22.585585531219937
40	20.185185596901654	26.00779986260198	30.93405562047148	22.87295892002489
41	21.15243462239524	24.911696522521556	31.0124417305036	22.923427124579604
42	19.928779168097236	26.729345911106723	30.517827917079693	22.824047003716352
43	20.286185527980315	26.384072247346925	29.938380513746633	23.391361710926127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	414.0
2	630.0
3	1929.5
4	3229.0
5	3229.0
6	4729.5
7	6230.0
8	6717.5
9	7205.0
10	10344.5
11	13484.0
12	13484.0
13	23928.5
14	34373.0
15	51601.5
16	68830.0
17	63926.5
18	59023.0
19	59023.0
20	62681.5
21	66340.0
22	53513.5
23	40687.0
24	45257.5
25	49828.0
26	49828.0
27	55622.5
28	61417.0
29	68158.5
30	74900.0
31	83112.0
32	91324.0
33	91324.0
34	102459.5
35	113595.0
36	126268.0
37	138941.0
38	149510.5
39	160080.0
40	160080.0
41	168029.5
42	175979.0
43	187710.0
44	199441.0
45	209605.5
46	219770.0
47	219770.0
48	265401.5
49	311033.0
50	295873.5
51	280714.0
52	260802.5
53	240891.0
54	240891.0
55	217851.0
56	194811.0
57	167300.0
58	139789.0
59	125551.0
60	111313.0
61	111313.0
62	98730.5
63	86148.0
64	74584.5
65	63021.0
66	53896.0
67	44771.0
68	44771.0
69	38746.0
70	32721.0
71	27926.5
72	23132.0
73	19024.5
74	14917.0
75	14917.0
76	11878.0
77	8839.0
78	6972.0
79	5105.0
80	3908.0
81	2711.0
82	2711.0
83	2083.0
84	1455.0
85	1115.0
86	775.0
87	604.0
88	433.0
89	433.0
90	356.5
91	280.0
92	187.0
93	94.0
94	66.0
95	38.0
96	38.0
97	27.5
98	17.0
99	11.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3148517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.35715259531764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26497396947451	36.28141341171494
2	11.772228114393245	10.914539501716323
3	3.877412197168848	5.392373666973064
4	1.8232773984899702	3.380877943415733
5	0.9698506366662552	2.2479756979301784
6	0.5922341627221892	1.6472573672083621
7	0.3961923882295596	1.285644569878272
8	0.30019093315533235	1.1132797516810031
9	0.23939672816920785	0.9987975592703773
>10	1.4982146580729776	13.27576391933196
>50	0.1537493636467953	4.944539522417071
>100	0.09970159801794685	8.846293045269356
>500	0.008110308806500637	2.549305213048632
>1k	0.003848960111489495	3.1246042959659936
>5k	3.4365715281156205E-4	0.9101680108350879
>10k+	2.7492572224924964E-4	3.0871665233436025
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32627	1.0362656450640095	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28203	0.8957550491231269	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23190	0.7365372332434603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12872	0.4088273939762752	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6094	0.1935514402494889	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5928	0.1882791168032442	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5633	0.17890962634154428	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5632	0.17887786535692834	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5279	0.1676662377875044	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4644	0.14749801255638767	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3449	0.10954363594034906	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3388	0.10760621587877722	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3340	0.10608168861721248	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3198	0.10157162880175016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.352196923186376E-5	0.0
5	0.0	0.0	0.0	6.352196923186376E-5	0.0
6	0.0	0.0	0.0	6.352196923186376E-5	0.0
7	0.0	0.0	0.0	6.352196923186376E-5	0.0
8	0.0	0.0	0.0	9.528295384779564E-5	0.0
9	3.176098461593188E-5	0.0	0.0	4.1289280000711447E-4	0.0
10	3.176098461593188E-5	0.0	0.0	5.39936738470842E-4	0.0
11	3.176098461593188E-5	0.0	0.0	7.94024615398297E-4	0.0
12	3.176098461593188E-5	0.0	0.0	0.0012386784000213433	0.0
13	6.352196923186376E-5	0.0	0.0	0.001302200369253207	0.0
14	1.2704393846372752E-4	0.0	0.0	0.0015245272615647303	3.176098461593188E-5
15	1.2704393846372752E-4	0.0	0.0	0.0019374200615718449	3.176098461593188E-5
16	1.2704393846372752E-4	0.0	0.0	0.002636161723122346	3.176098461593188E-5
17	1.2704393846372752E-4	0.0	0.0	0.0035254692923684387	3.176098461593188E-5
18	1.2704393846372752E-4	0.0	0.0	0.004001884061607417	3.176098461593188E-5
19	1.5880492307965941E-4	0.0	0.0	0.0049229526154694414	3.176098461593188E-5
20	1.5880492307965941E-4	0.0	0.0	0.008162573046294494	3.176098461593188E-5
21	1.5880492307965941E-4	0.0	0.0	0.014578291938712734	3.176098461593188E-5
22	1.5880492307965941E-4	0.0	0.0	0.024519480123499412	6.352196923186376E-5
23	1.5880492307965941E-4	0.0	0.0	0.032142116431323066	6.352196923186376E-5
24	1.5880492307965941E-4	0.0	0.0	0.0445289004315365	6.352196923186376E-5
25	1.5880492307965941E-4	0.0	0.0	0.054279522708627584	6.352196923186376E-5
26	1.5880492307965941E-4	0.0	0.0	0.06609460898575424	6.352196923186376E-5
27	1.5880492307965941E-4	0.0	0.0	0.12199394190979436	1.2704393846372752E-4
28	1.5880492307965941E-4	0.0	0.0	0.25062592960431845	1.2704393846372752E-4
29	2.2232689231152316E-4	0.0	0.0	0.4206107192687859	1.2704393846372752E-4
30	2.2232689231152316E-4	0.0	0.0	0.6599614993344486	1.2704393846372752E-4
31	2.2232689231152316E-4	0.0	0.0	1.0429036908487392	1.2704393846372752E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11465	0.0	18.895334	1
TAATACT	895	0.0	17.156425	4
GTATTGG	1470	0.0	16.989796	1
CTCTAAT	730	0.0	16.979452	1
CTAATAC	875	0.0	16.279999	3
AATACTG	890	0.0	16.213482	5
TGCGACG	155	7.223207E-9	15.5161295	22
TCTAATA	825	0.0	14.8	2
TACTGGT	865	0.0	14.757225	7
ATACTGG	885	0.0	14.63277	6
ATTGGAC	1710	0.0	14.605263	3
GCTAGAG	735	0.0	14.598639	17
GACCGTG	330	0.0	14.575759	7
TCTAGTA	305	0.0	14.557377	2
AAGACCG	210	1.364242E-10	14.095239	5
TCTACGC	105	1.6570745E-4	14.095239	3
TGCTAGA	775	0.0	14.083872	16
GGTAAAC	750	0.0	14.059999	35
TATACCG	175	3.5723133E-8	13.742857	5
TTAACGG	475	0.0	13.63158	35
>>END_MODULE