FastQCFastQC Report
Wed 25 May 2016
SRR2088629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4436396
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT422270.9518311710676864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT373180.8411782897649354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT297350.6702512580031178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164660.37115712844389903No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95160.21449843521633327No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG92090.20757840373131706No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA81990.1848121763701888No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG73860.16648649038543897No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA67310.15172225382945975No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG59880.13497442518657035No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57120.1287531590958066No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG46710.10528816634042587No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44580.10048697185733645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153650.018.8190691
ACCGTTA5850.016.4444458
GTATTGG24850.015.7826961
CTAATAC10400.015.2980763
GTATTAG11800.014.8940681
TGTTACG3550.014.5915516
GACCGTT6650.014.4661667
CCGTTTA6450.014.34108527
ATTGGAC29050.014.3287433
TTGGACC33400.014.0688624
ATTAGAC3200.013.8750013
ATTAGAG9750.013.8512823
TTAACGG7100.013.80985935
TAATACT12250.013.7428574
TGGACCC31750.013.5763785
GCGCTAG1501.3077952E-613.56666711
CGTTATT3550.013.54929610
TAACGGC7250.013.52413836
CTCTAAT10000.013.5051
CGTTTAC6750.013.42962928
Produced by FastQC (version 0.11.5)