##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4436396
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76617844755067	31.0	31.0	34.0	30.0	34.0
2	31.943621353909794	31.0	31.0	34.0	30.0	34.0
3	32.032198433142575	33.0	31.0	34.0	30.0	34.0
4	35.70063650765171	37.0	35.0	37.0	35.0	37.0
5	35.61161244397479	37.0	35.0	37.0	33.0	37.0
6	35.64671954442299	37.0	35.0	37.0	33.0	37.0
7	35.52247206967097	37.0	35.0	37.0	33.0	37.0
8	35.54132656327343	37.0	35.0	37.0	33.0	37.0
9	37.17262818738453	39.0	37.0	39.0	33.0	39.0
10	37.03241798072129	39.0	37.0	39.0	33.0	39.0
11	37.07977150822424	39.0	37.0	39.0	33.0	39.0
12	36.98700611938159	39.0	37.0	39.0	33.0	39.0
13	37.02892077262715	39.0	37.0	39.0	33.0	39.0
14	38.10108340193256	40.0	37.0	41.0	33.0	41.0
15	38.065052804122985	40.0	37.0	41.0	33.0	41.0
16	37.97345638216246	40.0	37.0	41.0	33.0	41.0
17	37.9779548534441	40.0	37.0	41.0	33.0	41.0
18	37.951678569721906	40.0	37.0	41.0	33.0	41.0
19	37.96814734302348	40.0	37.0	41.0	33.0	41.0
20	37.84996267240346	40.0	37.0	41.0	32.0	41.0
21	37.75128640455	40.0	37.0	41.0	32.0	41.0
22	37.59756432924383	39.0	37.0	41.0	32.0	41.0
23	37.46841580417979	39.0	36.0	41.0	32.0	41.0
24	37.335608002531785	39.0	36.0	41.0	31.0	41.0
25	37.14249967766629	39.0	36.0	41.0	31.0	41.0
26	37.30243107242906	39.0	36.0	41.0	31.0	41.0
27	37.239647903388246	39.0	36.0	41.0	31.0	41.0
28	37.13228733413338	39.0	36.0	41.0	31.0	41.0
29	36.99097984039297	39.0	36.0	41.0	31.0	41.0
30	36.86863683945256	39.0	35.0	41.0	30.0	41.0
31	36.70212983692168	39.0	35.0	41.0	30.0	41.0
32	36.58042068381632	39.0	35.0	40.0	30.0	41.0
33	36.38768991767191	39.0	35.0	40.0	30.0	41.0
34	36.250784420507095	39.0	35.0	40.0	30.0	41.0
35	36.064984505440904	38.0	35.0	40.0	29.0	41.0
36	35.92479616337225	38.0	35.0	40.0	28.0	41.0
37	35.77013819325416	38.0	35.0	40.0	27.0	41.0
38	35.69278035594658	38.0	35.0	40.0	27.0	41.0
39	35.565151532910946	38.0	35.0	40.0	26.0	41.0
40	35.39746091196548	38.0	34.0	40.0	25.0	41.0
41	35.27748672571159	38.0	34.0	40.0	25.0	41.0
42	35.072901517357785	38.0	34.0	40.0	24.0	41.0
43	34.73248488187258	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	5.0
14	9.0
15	30.0
16	61.0
17	142.0
18	308.0
19	678.0
20	1432.0
21	2795.0
22	5381.0
23	9131.0
24	14638.0
25	22697.0
26	33601.0
27	47316.0
28	64882.0
29	85575.0
30	107679.0
31	134263.0
32	166189.0
33	207221.0
34	259304.0
35	325547.0
36	427049.0
37	613237.0
38	868258.0
39	1038964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28553988417625	20.657375942093537	13.237794822644327	23.81928935108588
2	18.87338280892869	22.566583325744592	35.11273565299401	23.44729821233271
3	19.93419884068059	24.659746334637394	31.547026009400426	23.859028815281594
4	13.950242494132626	15.933406305478591	36.07489051924129	34.04146068114749
5	13.475600464881854	37.71396872596585	34.836092179327544	13.97433862982475
6	33.08665412194944	36.51610451366379	14.117923647934044	16.279317716452724
7	26.94768456197328	31.9120069533919	21.987149028175125	19.153159456459704
8	26.880174808560824	34.3928495111798	19.263541847932423	19.46343383232696
9	24.768393083034066	14.44862000596881	21.62000416554338	39.162982745453746
10	16.610216941860013	26.857949560859762	33.85265877978431	22.679174717495915
11	33.60716671821001	22.06536567069306	23.43855237449497	20.888915236601964
12	21.031395754571953	26.357160181372446	31.092783421497987	21.518660642557606
13	31.026130219213975	20.501303310164374	26.22297468485681	22.249591785764842
14	20.831436147719906	22.089213857374318	27.547969117274473	29.531380877631303
15	23.561737951255928	28.723292510407095	24.977436640011398	22.737532898325576
16	22.551819089188612	27.59622901111623	26.333018964041983	23.518932935653176
17	22.191323768211856	26.297066357466736	27.10091705068709	24.41069282363432
18	23.10057533186848	25.412113796874756	28.984585686219173	22.502725185037583
19	23.166777717769108	24.77456926748649	29.769434468879695	22.289218545864706
20	23.880059399566676	24.511901101705078	29.537286572253695	22.07075292647455
21	22.921533605205667	25.344649125100645	29.84127205957268	21.89254521012101
22	22.082699560634353	25.725814377255773	29.417369414272304	22.77411664783757
23	22.63075703791997	25.764697290323046	30.22710326129588	21.377442410461104
24	22.837073155777798	25.851592148221215	28.22115068176962	23.090184014231372
25	22.867052445273146	25.755320309548562	29.00031917799944	22.377308067178856
26	22.64869952997884	26.35794911004338	29.150238166295345	21.84311319368244
27	21.91706962137735	26.42978670073636	28.78092938502334	22.872214292862946
28	22.13533237339498	26.198698222611327	29.088566485047775	22.577402918945918
29	22.510005869629314	26.185196271928834	28.611760537156737	22.693037321285114
30	22.262552756787265	26.28962788714082	29.798985482810824	21.64883387326109
31	22.75524998219275	25.881684141812407	29.75602268147388	21.60704319452096
32	22.297986924521616	25.77330788324577	28.935491782068144	22.99321341016447
33	22.103482195908573	25.891106204225235	29.40657236189015	22.59883923797605
34	21.797535657321845	26.22782096097824	29.65675291385169	22.317890467848226
35	21.555920616644684	27.703500769543567	28.84778545467988	21.89279315913187
36	23.03518441545795	25.94966725242742	28.867125477527257	22.148022854587374
37	22.078619672364685	26.154473135400895	28.921268525172234	22.845638667062182
38	21.979552772115024	25.245401898297626	29.96179781967164	22.81324750991571
39	21.545754707199265	25.16285741849916	30.445501258228525	22.845886616073045
40	20.8663969582517	25.85903061854713	30.206523493394187	23.068048929806988
41	21.843654173342504	24.71720288270028	30.31176657809627	23.12737636586094
42	20.52352855786544	26.464364317342277	29.861671500921016	23.150435623871267
43	20.990709575971124	26.10317023097127	29.340753169915402	23.56536702314221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	216.0
1	490.0
2	764.0
3	2460.5
4	4157.0
5	4157.0
6	6274.0
7	8391.0
8	8892.0
9	9393.0
10	13369.5
11	17346.0
12	17346.0
13	30448.0
14	43550.0
15	65976.0
16	88402.0
17	82211.0
18	76020.0
19	76020.0
20	82191.0
21	88362.0
22	71686.0
23	55010.0
24	61908.0
25	68806.0
26	68806.0
27	76856.0
28	84906.0
29	94016.5
30	103127.0
31	114044.0
32	124961.0
33	124961.0
34	140566.0
35	156171.0
36	172806.0
37	189441.0
38	204448.5
39	219456.0
40	219456.0
41	228297.5
42	237139.0
43	255773.5
44	274408.0
45	286589.5
46	298771.0
47	298771.0
48	364120.0
49	429469.0
50	414529.5
51	399590.0
52	376253.0
53	352916.0
54	352916.0
55	319387.0
56	285858.0
57	246936.0
58	208014.0
59	187849.5
60	167685.0
61	167685.0
62	149490.0
63	131295.0
64	114771.0
65	98247.0
66	84374.0
67	70501.0
68	70501.0
69	61199.5
70	51898.0
71	44099.0
72	36300.0
73	29775.5
74	23251.0
75	23251.0
76	18866.5
77	14482.0
78	11295.0
79	8108.0
80	6384.0
81	4660.0
82	4660.0
83	3571.0
84	2482.0
85	1918.5
86	1355.0
87	1040.0
88	725.0
89	725.0
90	607.5
91	490.0
92	324.0
93	158.0
94	118.5
95	79.0
96	79.0
97	52.5
98	26.0
99	18.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4436396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.43279342132551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08243758653771	33.04472794686985
2	12.443728319601629	10.809317629927111
3	4.5064208169822715	5.871793332406558
4	2.1373029879794956	3.713161566227808
5	1.1669759890367963	2.53425135297411
6	0.6978836878332519	1.8186622827464676
7	0.49541600138000047	1.5062110591889672
8	0.338097235890552	1.1747605922204407
9	0.25394787376739714	0.9926698987011789
>10	1.5858778594931517	13.153868029984913
>50	0.1634572647131349	4.956072694284828
>100	0.113164347660901	9.503700966188584
>500	0.009673283810973056	2.909318287679773
>1k	0.005044669401169457	3.979811099020426
>5k	3.640483073008881E-4	1.191316339503357
>10k+	2.0802760417193606E-4	2.840356922075599
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42227	0.9518311710676864	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37318	0.8411782897649354	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29735	0.6702512580031178	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16466	0.37115712844389903	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9516	0.21449843521633327	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9209	0.20757840373131706	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8199	0.1848121763701888	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7386	0.16648649038543897	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6731	0.15172225382945975	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5988	0.13497442518657035	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5712	0.1287531590958066	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4671	0.10528816634042587	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4458	0.10048697185733645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.2540819169433926E-5	0.0	0.0	0.0	0.0
3	2.2540819169433926E-5	0.0	0.0	0.0	0.0
4	2.2540819169433926E-5	0.0	0.0	0.0	0.0
5	2.2540819169433926E-5	0.0	0.0	0.0	0.0
6	2.2540819169433926E-5	0.0	0.0	0.0	0.0
7	2.2540819169433926E-5	0.0	2.2540819169433926E-5	2.2540819169433926E-5	0.0
8	6.762245750830179E-5	0.0	2.2540819169433926E-5	4.508163833886785E-5	0.0
9	1.3524491501660358E-4	0.0	2.2540819169433926E-5	2.479490108637732E-4	0.0
10	2.0286737252490534E-4	0.0	2.2540819169433926E-5	3.3811228754150895E-4	2.2540819169433926E-5
11	2.0286737252490534E-4	0.0	2.2540819169433926E-5	4.7335720255811247E-4	2.2540819169433926E-5
12	2.0286737252490534E-4	0.0	2.2540819169433926E-5	6.086021175747161E-4	2.2540819169433926E-5
13	2.0286737252490534E-4	0.0	2.2540819169433926E-5	6.53683755913584E-4	2.2540819169433926E-5
14	2.7048983003320716E-4	0.0	2.2540819169433926E-5	8.790919476079231E-4	2.2540819169433926E-5
15	2.7048983003320716E-4	0.0	2.2540819169433926E-5	0.0011270409584716965	2.2540819169433926E-5
16	2.7048983003320716E-4	0.0	2.2540819169433926E-5	0.0017807247143852803	2.2540819169433926E-5
17	2.7048983003320716E-4	0.0	2.2540819169433926E-5	0.0023893268319599963	2.2540819169433926E-5
18	2.930306492026411E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.0028626840345181087	2.2540819169433926E-5
19	2.930306492026411E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.003809398439634334	2.2540819169433926E-5
20	2.930306492026411E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.0061761844524248965	2.2540819169433926E-5
21	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.010571644190464512	2.2540819169433926E-5
22	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.017762165505513936	2.2540819169433926E-5
23	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.023600237670397324	2.2540819169433926E-5
24	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.03367598383913429	2.2540819169433926E-5
25	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.04133986235674182	2.2540819169433926E-5
26	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.0518213432705286	2.2540819169433926E-5
27	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.09830051239790136	2.2540819169433926E-5
28	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.2039718726642076	2.2540819169433926E-5
29	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.33396477681433306	2.2540819169433926E-5
30	3.3811228754150895E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.5082503906323962	2.2540819169433926E-5
31	3.606531067109428E-4	2.2540819169433926E-5	2.2540819169433926E-5	0.7842627213621146	2.2540819169433926E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15365	0.0	18.819069	1
ACCGTTA	585	0.0	16.444445	8
GTATTGG	2485	0.0	15.782696	1
CTAATAC	1040	0.0	15.298076	3
GTATTAG	1180	0.0	14.894068	1
TGTTACG	355	0.0	14.59155	16
GACCGTT	665	0.0	14.466166	7
CCGTTTA	645	0.0	14.341085	27
ATTGGAC	2905	0.0	14.328743	3
TTGGACC	3340	0.0	14.068862	4
ATTAGAC	320	0.0	13.875001	3
ATTAGAG	975	0.0	13.851282	3
TTAACGG	710	0.0	13.809859	35
TAATACT	1225	0.0	13.742857	4
TGGACCC	3175	0.0	13.576378	5
GCGCTAG	150	1.3077952E-6	13.566667	11
CGTTATT	355	0.0	13.549296	10
TAACGGC	725	0.0	13.524138	36
CTCTAAT	1000	0.0	13.505	1
CGTTTAC	675	0.0	13.429629	28
>>END_MODULE