FastQCFastQC Report
Wed 25 May 2016
SRR2088628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3462914
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT547071.5797966683550329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT486801.4057524963080226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT386261.1154189795068545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205340.5929688118157136No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG113400.327469870750472No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA104530.30185560484609203No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG104180.30084489536846715No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA99300.2867527175090112No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA95320.27525950687773365No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG83360.240722120156608No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58950.17023235344568188No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57990.16746012173562497No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54910.15856587833252572No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA54800.15824822678241504No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC53240.15374335025357255No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.15339682128981547No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.14918071889743725No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG47200.1363013924111312No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45830.13234518674157084No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44770.12928418089504964No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC39810.11496098372642233No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC39310.11351711304410102No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35500.10251481844481267No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35030.10115758000343064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169150.021.5131531
GTATTGG19450.018.4524421
ATTGGAC21050.017.3135383
TTGGACC27450.015.9052824
TGTTACG4000.015.72516
TAATACT7200.015.6736124
TATTGGA22600.015.5530982
ACGTTTA1201.9381423E-615.41666726
TCTATAC3600.015.4166673
ACCGTTA6650.015.3007528
GACCGTT7150.014.7482527
GGACCCT28900.014.6591696
TGGACCC29200.014.6352745
GTATCAA254700.014.2726742
TATACAC19450.014.17223637
CGTTATT4750.014.02105210
CCGTTTA7200.013.87500127
AATACTG7350.013.8435375
ATACACA13150.013.78707237
GTATTAG6850.013.7737231
Produced by FastQC (version 0.11.5)