##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3462914
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.733339898132037	31.0	31.0	34.0	30.0	34.0
2	31.900294665128847	31.0	31.0	34.0	30.0	34.0
3	31.96709072186026	33.0	31.0	34.0	30.0	34.0
4	35.63524823313545	37.0	35.0	37.0	33.0	37.0
5	35.57287995023844	37.0	35.0	37.0	33.0	37.0
6	35.62909590015808	37.0	35.0	37.0	33.0	37.0
7	35.47928276590178	37.0	35.0	37.0	33.0	37.0
8	35.52824355441689	37.0	35.0	37.0	33.0	37.0
9	37.124739453535376	39.0	37.0	39.0	33.0	39.0
10	37.00994191597019	39.0	37.0	39.0	33.0	39.0
11	37.0589154105473	39.0	37.0	39.0	33.0	39.0
12	36.96038509763742	39.0	37.0	39.0	33.0	39.0
13	37.00640703176573	39.0	37.0	39.0	33.0	39.0
14	37.998518877454075	40.0	37.0	41.0	33.0	41.0
15	37.96918375680135	40.0	37.0	41.0	33.0	41.0
16	37.86772787311495	40.0	37.0	41.0	32.0	41.0
17	37.946082403432484	40.0	37.0	41.0	33.0	41.0
18	37.942273183798385	40.0	37.0	41.0	33.0	41.0
19	37.981716265549764	40.0	37.0	41.0	33.0	41.0
20	37.878340611404155	40.0	37.0	41.0	32.0	41.0
21	37.78283751776683	40.0	37.0	41.0	32.0	41.0
22	37.63121088193354	39.0	37.0	41.0	32.0	41.0
23	37.49323719849814	39.0	36.0	41.0	32.0	41.0
24	37.346021587599346	39.0	36.0	41.0	31.0	41.0
25	37.135026165824506	39.0	36.0	41.0	31.0	41.0
26	37.26094959331938	39.0	36.0	41.0	31.0	41.0
27	37.179552538700065	39.0	36.0	41.0	31.0	41.0
28	37.046784297848575	39.0	35.0	41.0	31.0	41.0
29	36.8812046732896	39.0	35.0	41.0	31.0	41.0
30	36.713215517335975	39.0	35.0	41.0	30.0	41.0
31	36.470076068883024	39.0	35.0	40.0	30.0	41.0
32	36.33112632886638	38.0	35.0	40.0	30.0	41.0
33	36.10307041988337	38.0	35.0	40.0	30.0	41.0
34	35.94016339995738	38.0	35.0	40.0	29.0	41.0
35	35.71754250899676	38.0	35.0	40.0	28.0	41.0
36	35.54598641490952	38.0	35.0	40.0	27.0	41.0
37	35.34641691939216	38.0	34.0	40.0	26.0	41.0
38	35.203210071055764	38.0	34.0	40.0	25.0	41.0
39	35.026613713190685	38.0	34.0	40.0	24.0	41.0
40	34.78902652505953	38.0	34.0	40.0	23.0	41.0
41	34.60295433268051	38.0	33.0	40.0	22.0	41.0
42	34.315419614809954	38.0	33.0	40.0	21.0	41.0
43	33.92445812977163	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	10.0
13	12.0
14	13.0
15	35.0
16	58.0
17	120.0
18	230.0
19	558.0
20	1175.0
21	2427.0
22	4312.0
23	7660.0
24	12706.0
25	19516.0
26	28784.0
27	40641.0
28	55323.0
29	72762.0
30	91134.0
31	111029.0
32	137258.0
33	170491.0
34	211456.0
35	261703.0
36	342759.0
37	502276.0
38	642754.0
39	745705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37434137838826	20.997431642830286	14.366628798751572	22.261598180029882
2	19.06804500487162	23.61164614541395	34.94178602183017	22.37852282788426
3	20.30786788236728	24.63029691179163	33.006161862523875	22.055673343317217
4	13.719543713762455	16.017492782090457	35.85535187994851	34.40761162419858
5	12.80701744253539	38.520390630549876	34.46805205096055	14.20453987595418
6	32.19002839804858	36.483002465553575	13.185340438717219	18.141628697680623
7	25.47441259009031	33.35439459368612	21.335701666284525	19.83549114993904
8	27.390082456566926	33.946497083092446	18.74404042375872	19.919380036581906
9	24.12771440468923	14.48300477574667	22.49868174606704	38.890599073497064
10	17.19072434371746	27.119241193977096	32.687788377072025	23.00224608523342
11	32.419834855846844	22.40670718360317	23.771136100983163	21.40232185956683
12	21.146612361727723	27.47862060680687	30.853148533287282	20.521618498178125
13	32.09759179696637	20.580008628571196	26.77554221675733	20.5468573577051
14	20.290368169697544	23.4253868273945	28.346531273950205	27.937713728957753
15	22.73293532556685	29.17574620680733	26.357397267157083	21.73392120046874
16	21.250253399304746	28.15380341527396	27.432301235318	23.163641950103294
17	21.39172384875859	26.43294058125613	27.45185124435663	24.723484325628647
18	22.904755936763085	24.75472968719408	30.37603590502103	21.964478471021803
19	22.583581342187532	24.31862298630575	31.725852851095926	21.371942820410787
20	23.746128260765357	23.74843845385707	31.54167270685902	20.963760578518553
21	22.31955514921826	24.858197460289226	32.19404235854543	20.628205031947083
22	20.753099845967878	25.31821465967679	31.741533286705938	22.187152207649397
23	21.80550830889823	25.58836286433911	32.84955964831931	19.756569178443357
24	21.85584164088395	25.616229568507908	30.064361979535153	22.463566811072987
25	21.706170005954522	25.89763996449233	31.169818251333993	21.226371778219153
26	21.45360814620288	26.703204295573034	31.198955561703233	20.644231996520848
27	20.28609431247787	26.768380618173023	30.91064346385732	22.03488160549179
28	20.724078045253215	26.376658502059247	30.981653024013877	21.917610428673655
29	21.02327692804384	26.238364568106515	30.328734701468186	22.40962380238146
30	20.96644617798767	26.453992215804377	32.31241087708213	20.26715072912582
31	21.68390552003313	26.102496336900078	32.02756406887379	20.186034074193007
32	21.4053539879997	25.906418698240845	30.722564868778147	21.96566244498131
33	20.858155876813576	26.049910566649935	31.38784272436451	21.70409083217198
34	20.089265861063833	26.667136405928648	31.956843282853686	21.28675445015383
35	19.651051108979317	28.939211311629453	30.703014859739515	20.706722719651715
36	22.02058728573681	26.18346860476466	30.6513820441397	21.144562065358826
37	20.979527646369505	26.895816644594696	30.562324100454124	21.562331608581676
38	20.686970568717562	25.326386967738728	32.169871963323374	21.816770500220333
39	20.17685105665344	25.100969876814727	32.90751661750768	21.814662449024144
40	18.980806338245767	26.018549695429915	32.4007757628402	22.599868203484117
41	20.56762021811688	24.84046672831032	32.35116436619564	22.240748687377163
42	19.09856843109589	27.04589256331517	31.65998347056843	22.195555535020507
43	19.383819523095287	27.00973804142985	30.733798182686606	22.87264425278826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	483.5
2	739.0
3	2295.0
4	3851.0
5	3851.0
6	6071.5
7	8292.0
8	9079.0
9	9866.0
10	14571.5
11	19277.0
12	19277.0
13	35937.5
14	52598.0
15	81646.5
16	110695.0
17	100313.5
18	89932.0
19	89932.0
20	95285.0
21	100638.0
22	76030.0
23	51422.0
24	56193.0
25	60964.0
26	60964.0
27	64050.0
28	67136.0
29	71252.0
30	75368.0
31	81867.5
32	88367.0
33	88367.0
34	97043.0
35	105719.0
36	118348.0
37	130977.0
38	139351.5
39	147726.0
40	147726.0
41	155120.0
42	162514.0
43	177007.0
44	191500.0
45	210380.0
46	229260.0
47	229260.0
48	310853.0
49	392446.0
50	368589.5
51	344733.0
52	314812.5
53	284892.0
54	284892.0
55	248650.0
56	212408.0
57	178902.5
58	145397.0
59	127197.0
60	108997.0
61	108997.0
62	95249.0
63	81501.0
64	70939.0
65	60377.0
66	51863.5
67	43350.0
68	43350.0
69	36948.0
70	30546.0
71	25501.5
72	20457.0
73	16940.0
74	13423.0
75	13423.0
76	10552.5
77	7682.0
78	6132.5
79	4583.0
80	3495.5
81	2408.0
82	2408.0
83	1852.5
84	1297.0
85	958.0
86	619.0
87	499.5
88	380.0
89	380.0
90	305.5
91	231.0
92	147.5
93	64.0
94	49.5
95	35.0
96	35.0
97	24.0
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3462914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.14783161923312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.02222310138849	30.922752440135525
2	12.145273900835372	9.752128230804104
3	4.2301354787429215	5.094923007813447
4	1.9465195216297873	3.1259415199177165
5	1.049015953679273	2.105785793710235
6	0.6644201679832443	1.6005017417168328
7	0.43496731674402833	1.2224096214756222
8	0.3307307313429752	1.0622497370610877
9	0.23785695509212965	0.8594496884252083
>10	1.5935451177800974	12.505421268288583
>50	0.18776657279527467	5.2766187352786975
>100	0.1389527731338273	11.033804288640793
>500	0.010953667231072094	2.952594627174977
>1k	0.006413660418117219	4.933508945506814
>5k	7.206360020356426E-4	1.9171771821301131
>10k+	5.044452014249498E-4	5.634733171920268
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	54707	1.5797966683550329	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	48680	1.4057524963080226	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38626	1.1154189795068545	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20534	0.5929688118157136	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11340	0.327469870750472	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10453	0.30185560484609203	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10418	0.30084489536846715	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9930	0.2867527175090112	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9532	0.27525950687773365	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8336	0.240722120156608	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5895	0.17023235344568188	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5799	0.16746012173562497	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5491	0.15856587833252572	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5480	0.15824822678241504	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5324	0.15374335025357255	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5312	0.15339682128981547	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5166	0.14918071889743725	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4720	0.1363013924111312	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4583	0.13234518674157084	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4477	0.12928418089504964	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3981	0.11496098372642233	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3931	0.11351711304410102	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3550	0.10251481844481267	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3503	0.10115758000343064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8877413646426104E-5
2	0.0	0.0	0.0	0.0	2.8877413646426104E-5
3	0.0	0.0	0.0	2.8877413646426104E-5	2.8877413646426104E-5
4	0.0	0.0	0.0	2.8877413646426104E-5	2.8877413646426104E-5
5	0.0	0.0	0.0	5.775482729285221E-5	2.8877413646426104E-5
6	0.0	0.0	0.0	5.775482729285221E-5	1.732644818785566E-4
7	0.0	0.0	0.0	5.775482729285221E-5	1.732644818785566E-4
8	0.0	0.0	0.0	5.775482729285221E-5	2.3101930917140883E-4
9	2.8877413646426104E-5	0.0	0.0	1.443870682321305E-4	2.3101930917140883E-4
10	5.775482729285221E-5	0.0	0.0	4.6203861834281766E-4	2.3101930917140883E-4
11	5.775482729285221E-5	0.0	0.0	9.529546503320614E-4	2.598967228178349E-4
12	5.775482729285221E-5	0.0	0.0	0.0013861158550284528	2.598967228178349E-4
13	8.66322409392783E-5	0.0	0.0	0.001674889991492714	2.598967228178349E-4
14	1.732644818785566E-4	0.0	0.0	0.0019059093006641228	3.176515501106871E-4
15	1.732644818785566E-4	0.0	0.0	0.0023968253326533667	3.176515501106871E-4
16	2.0214189552498273E-4	0.0	0.0	0.0032631477420461494	3.176515501106871E-4
17	2.0214189552498273E-4	0.0	0.0	0.0045626313561353245	3.176515501106871E-4
18	2.0214189552498273E-4	0.0	0.0	0.005746605315638795	3.176515501106871E-4
19	2.0214189552498273E-4	0.0	0.0	0.007219353411606525	3.176515501106871E-4
20	2.0214189552498273E-4	0.0	0.0	0.01230177821337752	3.176515501106871E-4
21	2.0214189552498273E-4	0.0	0.0	0.020705105584487515	3.176515501106871E-4
22	2.0214189552498273E-4	0.0	0.0	0.03401759327548995	3.176515501106871E-4
23	2.0214189552498273E-4	0.0	0.0	0.0432583656423463	3.176515501106871E-4
24	2.3101930917140883E-4	0.0	0.0	0.05579116316489523	3.176515501106871E-4
25	2.3101930917140883E-4	0.0	0.0	0.06451214208611591	3.176515501106871E-4
26	2.598967228178349E-4	0.0	0.0	0.07857544253192543	3.176515501106871E-4
27	2.598967228178349E-4	0.0	0.0	0.14597532598268395	3.176515501106871E-4
28	2.598967228178349E-4	0.0	0.0	0.29749511538548173	3.176515501106871E-4
29	2.598967228178349E-4	0.0	0.0	0.5001568043561001	3.176515501106871E-4
30	2.598967228178349E-4	0.0	0.0	0.7826645420590866	3.176515501106871E-4
31	2.598967228178349E-4	0.0	0.0	1.1550965458570441	3.465289637571132E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16915	0.0	21.513153	1
GTATTGG	1945	0.0	18.452442	1
ATTGGAC	2105	0.0	17.313538	3
TTGGACC	2745	0.0	15.905282	4
TGTTACG	400	0.0	15.725	16
TAATACT	720	0.0	15.673612	4
TATTGGA	2260	0.0	15.553098	2
ACGTTTA	120	1.9381423E-6	15.416667	26
TCTATAC	360	0.0	15.416667	3
ACCGTTA	665	0.0	15.300752	8
GACCGTT	715	0.0	14.748252	7
GGACCCT	2890	0.0	14.659169	6
TGGACCC	2920	0.0	14.635274	5
GTATCAA	25470	0.0	14.272674	2
TATACAC	1945	0.0	14.172236	37
CGTTATT	475	0.0	14.021052	10
CCGTTTA	720	0.0	13.875001	27
AATACTG	735	0.0	13.843537	5
ATACACA	1315	0.0	13.787072	37
GTATTAG	685	0.0	13.773723	1
>>END_MODULE