FastQCFastQC Report
Wed 25 May 2016
SRR2088627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269713
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135271.0653588645623067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127321.0027462899096096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97450.767496276717652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.40623353466492035No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30070.23682517230271724No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29360.23123335745952037No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA28110.22138861301727242No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG26780.21091380493072054No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26510.208787340131195No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG21570.16988091009543102No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16200.12758788797153373No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.11963333446219737No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14970.11790065944036172No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.11112747526409511No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC13040.10270037402153084No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.10191279446615101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.022.8772321
TTATACC1155.456968E-1222.5217384
AGTACCG1409.458745E-1118.55
TCGGGTA1552.1827873E-1117.90322725
CGTAAGG1258.580173E-917.763
TTAACGG2050.017.14634335
CCGTTTA2400.016.95833227
ACCTAAG904.4465458E-516.4444451
CTAATAC3150.016.4444453
CTATCCT1606.2937033E-1016.18754
TCTAACG803.3829155E-416.18752
AGTTCCG1606.2937033E-1016.187532
CGGGTAA1951.8189894E-1216.12820626
CGTTTAC2550.015.96078428
GTATTGG5950.015.8571421
GTATTAG3500.015.8571421
ATTACAC700.00259269415.8571423
TCTAATA2800.015.8571422
TACCGTG1403.473542E-815.8571427
GTAAGGT1302.589386E-715.6538464
Produced by FastQC (version 0.11.5)