##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1269713
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72470786705342	31.0	31.0	34.0	30.0	34.0
2	31.91478231694879	31.0	31.0	34.0	30.0	34.0
3	31.98734123380638	33.0	31.0	34.0	30.0	34.0
4	35.670839788204106	37.0	35.0	37.0	33.0	37.0
5	35.58454075842336	37.0	35.0	37.0	33.0	37.0
6	35.61903280505122	37.0	35.0	37.0	33.0	37.0
7	35.4942353114444	37.0	35.0	37.0	33.0	37.0
8	35.51649152209988	37.0	35.0	37.0	33.0	37.0
9	37.128912596783685	39.0	37.0	39.0	33.0	39.0
10	36.991158631911304	39.0	37.0	39.0	33.0	39.0
11	37.04282227558511	39.0	37.0	39.0	33.0	39.0
12	36.94132217280598	39.0	37.0	39.0	33.0	39.0
13	36.98613466192754	39.0	37.0	39.0	33.0	39.0
14	38.0252159346246	40.0	37.0	41.0	33.0	41.0
15	37.983478943666796	40.0	37.0	41.0	33.0	41.0
16	37.88674842267505	40.0	37.0	41.0	33.0	41.0
17	37.919183311504256	40.0	37.0	41.0	33.0	41.0
18	37.896239543896925	40.0	37.0	41.0	33.0	41.0
19	37.92138459636154	40.0	37.0	41.0	32.0	41.0
20	37.798709629656464	40.0	37.0	41.0	32.0	41.0
21	37.705770516644314	40.0	37.0	41.0	32.0	41.0
22	37.553813342070214	39.0	36.0	41.0	32.0	41.0
23	37.4246471446697	39.0	36.0	41.0	32.0	41.0
24	37.28324274855814	39.0	36.0	41.0	31.0	41.0
25	37.08539488845117	39.0	36.0	41.0	31.0	41.0
26	37.247256663513724	39.0	36.0	41.0	31.0	41.0
27	37.18463463790636	39.0	36.0	41.0	31.0	41.0
28	37.07088767304107	39.0	36.0	41.0	31.0	41.0
29	36.92713628985448	39.0	36.0	41.0	30.0	41.0
30	36.80423607539657	39.0	35.0	41.0	30.0	41.0
31	36.61667715460108	39.0	35.0	40.0	30.0	41.0
32	36.50202998630399	39.0	35.0	40.0	30.0	41.0
33	36.30935967419409	38.0	35.0	40.0	30.0	41.0
34	36.17679900890989	38.0	35.0	40.0	30.0	41.0
35	35.99244947480258	38.0	35.0	40.0	29.0	41.0
36	35.84807196586945	38.0	35.0	40.0	28.0	41.0
37	35.68654491211794	38.0	35.0	40.0	27.0	41.0
38	35.609965401630134	38.0	35.0	40.0	27.0	41.0
39	35.48014866351687	38.0	35.0	40.0	26.0	41.0
40	35.30024186568146	38.0	34.0	40.0	25.0	41.0
41	35.168035611197176	38.0	34.0	40.0	24.0	41.0
42	34.95317366995533	38.0	34.0	40.0	24.0	41.0
43	34.59625521673008	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	8.0
16	8.0
17	45.0
18	84.0
19	185.0
20	403.0
21	802.0
22	1512.0
23	2703.0
24	4264.0
25	6628.0
26	9792.0
27	13783.0
28	19013.0
29	24868.0
30	31702.0
31	39195.0
32	48596.0
33	60948.0
34	76075.0
35	95409.0
36	124741.0
37	178047.0
38	247023.0
39	283873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61609749604832	21.148558768792633	13.846908710866156	23.388435024292892
2	19.169922651811866	22.72041004541971	35.13132495296181	22.97834234980661
3	19.877011576631883	24.445996851256936	32.28501243981908	23.3919791322921
4	13.95890252364117	16.254460653706783	36.066339401108756	33.72029742154329
5	13.106977718586798	38.04938596359965	34.91922977869802	13.924406539115534
6	32.610597828013105	36.904954111677206	13.753974323331336	16.730473736978357
7	26.256563491119643	32.53002843949774	21.74389015470425	19.469517914678356
8	26.671617916804824	34.69287941448185	19.045327566150778	19.590175102562547
9	24.516879011241123	14.48941611214503	21.72687843630805	39.2668264403058
10	16.846484205485808	26.978301395669728	33.45937231484595	22.71584208399851
11	33.33005175185259	22.37482013651904	23.282820605916456	21.01230750571192
12	20.999784990781382	26.92135939381577	30.71851670416858	21.360338911234273
13	31.235562682275443	20.745711826215846	26.24002432045667	21.778701171052038
14	20.521015379065975	22.7975140838914	27.432971072990508	29.248499464052113
15	23.31259111310981	29.168402623269984	25.136704121325053	22.38230214229515
16	22.28078313760669	27.884332916178696	26.465350831250838	23.369533114963776
17	21.93062526728481	26.591836107844845	26.914979999417195	24.562558625453153
18	23.036386963038105	25.371166554961633	29.166906222114765	22.4255402598855
19	23.141607591636852	24.751420202833238	29.960707655982098	22.146264549547812
20	23.888311768092475	24.52837767274967	29.84674489431864	21.73656566483922
21	22.965504803053918	25.233261374814624	30.237620627653648	21.563613194477806
22	21.752632287768968	25.615158701218306	29.88297355386611	22.749235457146614
23	22.522176271330608	25.80772190250868	30.841300356852297	20.82880146930842
24	22.595657443847546	25.93893265643496	28.51148251612766	22.953927383589836
25	22.616843333887264	25.883172023914064	29.47036062480261	22.029624017396056
26	22.348830011191506	26.478109620047995	29.51816670381417	21.654893664946332
27	21.47753074907479	26.656181357519376	29.131622658033745	22.734665235372088
28	21.80957428962293	26.25483081609781	29.39191770108678	22.543677193192476
29	22.087117324938784	26.3093313213301	28.909603981372168	22.69394737235895
30	22.03112041855128	26.267904636717116	30.235179131031977	21.465795813699632
31	22.431210832684236	25.892622978578622	30.32078902870176	21.355377160035378
32	22.098852260313944	25.792836648911997	29.285988250888195	22.822322839885864
33	21.785710629094922	25.975948895537808	29.91542183154776	22.32291864381951
34	21.293000859249293	26.39509873491096	30.235966710587352	22.07593369525239
35	21.037037503750845	28.028853764590895	29.315680000126015	21.618428731532244
36	22.7185198544868	25.887818743290808	29.418852921880774	21.974808480341622
37	21.798075628114386	26.38832555073469	29.33064401167823	22.48295480947269
38	21.77247929256454	25.12118880408407	30.66653645351351	22.439795449837877
39	21.1700596906545	25.18584908558076	31.16846090415708	22.47563031960766
40	20.397759178649032	25.76826416678415	30.851381375161157	22.98259527940566
41	21.454297152191085	24.710229792086874	30.953687959405002	22.881785096317042
42	20.20180938527053	26.64554903352175	30.430498860766175	22.72214272044155
43	20.606073971047	26.319176065772343	29.664105195426053	23.4106447677546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	155.0
2	232.0
3	606.0
4	980.0
5	980.0
6	1554.5
7	2129.0
8	2353.0
9	2577.0
10	3761.0
11	4945.0
12	4945.0
13	9180.5
14	13416.0
15	20677.0
16	27938.0
17	25629.5
18	23321.0
19	23321.0
20	25511.0
21	27701.0
22	21918.0
23	16135.0
24	18308.0
25	20481.0
26	20481.0
27	22586.5
28	24692.0
29	27260.5
30	29829.0
31	32949.0
32	36069.0
33	36069.0
34	40429.5
35	44790.0
36	49278.0
37	53766.0
38	57837.0
39	61908.0
40	61908.0
41	64962.0
42	68016.0
43	73276.5
44	78537.0
45	82830.0
46	87123.0
47	87123.0
48	108036.0
49	128949.0
50	122587.0
51	116225.0
52	108598.0
53	100971.0
54	100971.0
55	90557.0
56	80143.0
57	69025.5
58	57908.0
59	51783.0
60	45658.0
61	45658.0
62	40504.0
63	35350.0
64	30608.5
65	25867.0
66	22104.0
67	18341.0
68	18341.0
69	15811.0
70	13281.0
71	11227.0
72	9173.0
73	7439.0
74	5705.0
75	5705.0
76	4562.5
77	3420.0
78	2633.0
79	1846.0
80	1446.0
81	1046.0
82	1046.0
83	791.5
84	537.0
85	414.5
86	292.0
87	234.5
88	177.0
89	177.0
90	141.5
91	106.0
92	69.0
93	32.0
94	25.5
95	19.0
96	19.0
97	10.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1269713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.52134329394056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57196963630008	43.36790763724952
2	9.700015325563857	10.189156697408478
3	2.850436099370514	4.491261987374389
4	1.325984592541402	2.785699679493715
5	0.7543623973035499	1.9810063218409861
6	0.48002784354419054	1.5127024296860656
7	0.3574807938540741	1.3142760046500157
8	0.2513911344360425	1.0562720058214843
9	0.21170012158221208	1.0006897284889484
>10	1.2861200734427782	12.826752668050545
>50	0.12513714292711117	4.566873377520988
>100	0.07695760812530575	7.380442943843615
>500	0.005110461683981752	1.7643899365475884
>1k	0.002705538538578574	2.5130912411658133
>5k	3.0061539317539714E-4	1.1764967885623585
>10k+	3.0061539317539714E-4	2.0729805522954416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13527	1.0653588645623067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12732	1.0027462899096096	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9745	0.767496276717652	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5158	0.40623353466492035	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3007	0.23682517230271724	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2936	0.23123335745952037	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2811	0.22138861301727242	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2678	0.21091380493072054	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2651	0.208787340131195	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2157	0.16988091009543102	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1620	0.12758788797153373	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1519	0.11963333446219737	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1497	0.11790065944036172	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1411	0.11112747526409511	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1304	0.10270037402153084	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1294	0.10191279446615101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.5751591107596757E-4	0.0
10	0.0	0.0	0.0	2.3627386661395134E-4	0.0
11	0.0	0.0	0.0	6.300636443038703E-4	0.0
12	0.0	0.0	0.0	0.001496401155221692	0.0
13	0.0	0.0	0.0	0.0015751591107596755	0.0
14	7.875795553798379E-5	0.0	0.0	0.0017326750218356432	0.0
15	1.5751591107596757E-4	0.0	0.0	0.002126464799525562	0.0
16	1.5751591107596757E-4	0.0	0.0	0.0030715602659813672	0.0
17	1.5751591107596757E-4	0.0	0.0	0.004095413687975157	0.0
18	1.5751591107596757E-4	0.0	0.0	0.004725477332279027	0.0
19	1.5751591107596757E-4	0.0	0.0	0.005434298932120881	0.0
20	1.5751591107596757E-4	0.0	0.0	0.00937219670902007	0.0
21	1.5751591107596757E-4	0.0	0.0	0.016145380885286675	0.0
22	1.5751591107596757E-4	0.0	0.0	0.026856462838452468	0.0
23	1.5751591107596757E-4	0.0	0.0	0.03339337314810512	0.0
24	1.5751591107596757E-4	0.0	0.0	0.04394693919019495	0.0
25	1.5751591107596757E-4	0.0	0.0	0.05016881767769567	7.875795553798379E-5
26	2.3627386661395134E-4	0.0	0.0	0.06001356211994364	7.875795553798379E-5
27	3.1503182215193514E-4	0.0	0.0	0.11207257073055092	7.875795553798379E-5
28	3.1503182215193514E-4	0.0	0.0	0.2340686438588878	7.875795553798379E-5
29	3.1503182215193514E-4	0.0	0.0	0.3874891412468802	7.875795553798379E-5
30	3.1503182215193514E-4	0.0	0.0	0.5941500165785496	7.875795553798379E-5
31	3.1503182215193514E-4	0.0	0.0	0.8956354703779515	7.875795553798379E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4480	0.0	22.877232	1
TTATACC	115	5.456968E-12	22.521738	4
AGTACCG	140	9.458745E-11	18.5	5
TCGGGTA	155	2.1827873E-11	17.903227	25
CGTAAGG	125	8.580173E-9	17.76	3
TTAACGG	205	0.0	17.146343	35
CCGTTTA	240	0.0	16.958332	27
ACCTAAG	90	4.4465458E-5	16.444445	1
CTAATAC	315	0.0	16.444445	3
CTATCCT	160	6.2937033E-10	16.1875	4
TCTAACG	80	3.3829155E-4	16.1875	2
AGTTCCG	160	6.2937033E-10	16.1875	32
CGGGTAA	195	1.8189894E-12	16.128206	26
CGTTTAC	255	0.0	15.960784	28
GTATTGG	595	0.0	15.857142	1
GTATTAG	350	0.0	15.857142	1
ATTACAC	70	0.002592694	15.857142	3
TCTAATA	280	0.0	15.857142	2
TACCGTG	140	3.473542E-8	15.857142	7
GTAAGGT	130	2.589386E-7	15.653846	4
>>END_MODULE