FastQCFastQC Report
Wed 25 May 2016
SRR2088626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804527
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188391.0439854876097725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168560.9340951950289467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135910.7531613547483634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72230.4002710959714097No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40160.22255139435431No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG38470.21318605928312517No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA37390.20720111142698336No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG35650.19755869543653268No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA32550.18037967844205158No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG29180.161704424483535No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20580.11404650636981326No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.1127165179573373No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19990.11077695152247652No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.1063990729980765No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.10407159327624359No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC18490.10246452394450181No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG18400.10196577828982333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA652.6842554E-622.7692314
GGTATCA62500.020.66081
GTCGTAC500.007036399618.4999981
TTAACGG2100.017.61904735
GTGCGCG1401.87174E-917.17857211
TTAGAGT1452.9831426E-916.5862064
TAACGGC2300.016.08695636
CGTAATA700.002593260715.85714314
TTATGCG2400.015.4166674
ACCGTTA2500.014.8000018
CCGTTTA2900.014.67241427
ATTGGAC10100.014.103963
GTATCAA92300.013.9501632
CTAATAC3750.013.8133333
TACCCCG2304.0017767E-1113.6739135
TCTATAC1501.3065619E-613.5666673
TTGGACC13150.013.5057034
CCTATGT3600.013.3611128
TAGCGAA3050.013.34426210
CGCAATA1254.8473703E-513.32000128
Produced by FastQC (version 0.11.5)