##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1804527
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.684182059897136	31.0	31.0	34.0	30.0	34.0
2	31.866352235239482	31.0	31.0	34.0	30.0	34.0
3	31.948460732369202	31.0	31.0	34.0	30.0	34.0
4	35.630014402666184	37.0	35.0	37.0	33.0	37.0
5	35.53752922510996	37.0	35.0	37.0	33.0	37.0
6	35.57134085552613	37.0	35.0	37.0	33.0	37.0
7	35.434122071878114	37.0	35.0	37.0	33.0	37.0
8	35.46036773071281	37.0	35.0	37.0	33.0	37.0
9	37.062812027750205	39.0	37.0	39.0	33.0	39.0
10	36.931638595598734	39.0	37.0	39.0	33.0	39.0
11	36.96759981978657	39.0	37.0	39.0	33.0	39.0
12	36.87167828466961	39.0	37.0	39.0	33.0	39.0
13	36.92038578530551	39.0	37.0	39.0	33.0	39.0
14	37.95023571273802	40.0	37.0	41.0	33.0	41.0
15	37.907488222675525	40.0	37.0	41.0	33.0	41.0
16	37.80371698511577	40.0	37.0	41.0	32.0	41.0
17	37.83748982420324	40.0	37.0	41.0	32.0	41.0
18	37.81129403993401	40.0	37.0	41.0	32.0	41.0
19	37.829098151482356	40.0	37.0	41.0	32.0	41.0
20	37.71185357714238	40.0	37.0	41.0	32.0	41.0
21	37.61370043230165	39.0	37.0	41.0	32.0	41.0
22	37.45703112228301	39.0	36.0	41.0	32.0	41.0
23	37.31837428866401	39.0	36.0	41.0	31.0	41.0
24	37.171102455103195	39.0	36.0	41.0	31.0	41.0
25	36.97232127865086	39.0	36.0	41.0	31.0	41.0
26	37.12948767183866	39.0	36.0	41.0	31.0	41.0
27	37.05731418815014	39.0	36.0	41.0	31.0	41.0
28	36.93641879561791	39.0	36.0	41.0	31.0	41.0
29	36.78986293915248	39.0	35.0	41.0	30.0	41.0
30	36.664232233709996	39.0	35.0	40.0	30.0	41.0
31	36.47976561170877	39.0	35.0	40.0	30.0	41.0
32	36.35625845443155	38.0	35.0	40.0	30.0	41.0
33	36.14685787466743	38.0	35.0	40.0	30.0	41.0
34	36.003969461249405	38.0	35.0	40.0	29.0	41.0
35	35.81126854848944	38.0	35.0	40.0	28.0	41.0
36	35.68056837054807	38.0	35.0	40.0	27.0	41.0
37	35.505267585356165	38.0	34.0	40.0	26.0	41.0
38	35.42127050468073	38.0	34.0	40.0	26.0	41.0
39	35.28032387434491	38.0	34.0	40.0	25.0	41.0
40	35.10745253465313	38.0	34.0	40.0	24.0	41.0
41	34.980954565933345	38.0	34.0	40.0	24.0	41.0
42	34.7600839444353	38.0	33.0	40.0	23.0	41.0
43	34.403363873192255	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	3.0
14	5.0
15	12.0
16	29.0
17	50.0
18	126.0
19	280.0
20	629.0
21	1380.0
22	2467.0
23	4186.0
24	7021.0
25	10417.0
26	15269.0
27	21292.0
28	28645.0
29	38180.0
30	47670.0
31	58869.0
32	72152.0
33	88963.0
34	109386.0
35	135076.0
36	176246.0
37	250058.0
38	342689.0
39	393422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.995381615237676	20.8762185326127	13.565770974887048	23.562628877262572
2	19.01296018291774	22.768958292117546	35.10061085259461	23.1174706723701
3	19.90022870259076	24.681924958728796	31.919998980342218	23.497847358338223
4	13.973855752781752	16.031347826882058	36.12209736956	33.87269905077619
5	13.25726907937648	37.969839187776074	34.77603826376663	13.996853469080817
6	32.872547764594266	36.85292600221554	13.798463530886487	16.476062702303707
7	26.49918787582563	32.348476913894885	21.818182825748796	19.334152384530682
8	26.744903235030566	34.54833316431397	19.222433357882704	19.48433024277276
9	24.613984717324815	14.422671425808536	21.732564821695657	39.23077903517099
10	16.750594477112283	26.85163480513176	33.615900454800624	22.781870262955334
11	33.32867837388967	22.303517763934817	23.38363460341685	20.984169258758666
12	20.99841121800893	26.705502328310963	30.944119982687983	21.35196647099212
13	31.04331495178515	20.67605527653507	26.300132943425066	21.98049682825472
14	20.723269865177965	22.523963343302704	27.478225595959493	29.274541195559834
15	23.296298697664263	28.909625624886743	25.242293409852003	22.55178226759699
16	22.279245475407127	27.868909692124305	26.430083894560735	23.42176093790783
17	22.0458325090176	26.40320704539195	27.018770015632903	24.532190429957545
18	23.097243765263695	25.331845962958717	29.181829919973488	22.389080351804104
19	23.145234180480536	24.72786497514307	30.007531059385645	22.11936978499075
20	23.92377614743365	24.52332384054104	29.776556405085653	21.77634360693966
21	22.91852657233724	25.285130119970496	30.07070550897825	21.72563779871401
22	21.88074769731902	25.65248400273313	29.80869779172049	22.65807050822736
23	22.519807129513715	25.744585700297083	30.654902919158317	21.08070425103088
24	22.64770768184682	25.835966987470954	28.53645304281953	22.979872287862694
25	22.658292172962778	25.817513398247847	29.309619639938887	22.214574788850484
26	22.413795969802614	26.408526999041854	29.431424412048145	21.74625261910739
27	21.65027178867371	26.58148090884758	29.037858674322965	22.730388628155744
28	21.912224089747617	26.153834218052708	29.36326250590875	22.570679186290924
29	22.26350727919283	26.18292771457562	28.908849798312797	22.64471520791875
30	22.083183016934633	26.261397030911702	30.1257891957283	21.52963075642537
31	22.491600291932457	25.85375558248782	30.161255553394323	21.4933885721854
32	22.04810457255558	25.81330176827501	29.257528427116913	22.8810652320525
33	21.877090229184713	25.855528900371123	29.784758000295923	22.482622870148244
34	21.404722678020335	26.226651083635765	30.17084255320092	22.197783685142976
35	21.16743057876108	27.856385634573495	29.185044058636972	21.79113972802845
36	22.75344176063866	25.813080103539598	29.243785213521328	22.189692922300413
37	21.907347465568538	26.157048356716196	29.314828761221083	22.620775416494183
38	21.839850553635383	25.052603812522616	30.318083353698782	22.78946228014322
39	21.23304334044323	25.044568465863907	31.095627829342536	22.626760364350325
40	20.62562654922869	25.7151042904872	30.65091295392089	23.008356206363217
41	21.541212738850678	24.699713553745664	30.702339172536625	23.056734534867033
42	20.282157041706775	26.42742391773578	30.32124207617841	22.96917696437903
43	20.68486644976772	26.15328005621418	29.607537044333505	23.5543164496846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	221.5
2	348.0
3	950.0
4	1552.0
5	1552.0
6	2397.0
7	3242.0
8	3525.5
9	3809.0
10	5633.0
11	7457.0
12	7457.0
13	13302.5
14	19148.0
15	29187.0
16	39226.0
17	36277.0
18	33328.0
19	33328.0
20	35812.0
21	38296.0
22	30910.0
23	23524.0
24	26260.5
25	28997.0
26	28997.0
27	31810.5
28	34624.0
29	38293.0
30	41962.0
31	46460.5
32	50959.0
33	50959.0
34	57115.0
35	63271.0
36	70135.5
37	77000.0
38	82540.5
39	88081.0
40	88081.0
41	91802.0
42	95523.0
43	102617.0
44	109711.0
45	116047.0
46	122383.0
47	122383.0
48	150654.0
49	178925.0
50	170988.5
51	163052.0
52	152541.5
53	142031.0
54	142031.0
55	127942.0
56	113853.0
57	98978.5
58	84104.0
59	75348.0
60	66592.0
61	66592.0
62	59088.0
63	51584.0
64	44854.5
65	38125.0
66	33013.5
67	27902.0
68	27902.0
69	24027.0
70	20152.0
71	16991.5
72	13831.0
73	11387.5
74	8944.0
75	8944.0
76	7195.0
77	5446.0
78	4332.0
79	3218.0
80	2643.0
81	2068.0
82	2068.0
83	1537.0
84	1006.0
85	784.5
86	563.0
87	435.5
88	308.0
89	308.0
90	246.0
91	184.0
92	123.5
93	63.0
94	46.5
95	30.0
96	30.0
97	18.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1804527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.844931398963645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1415840036488	41.2563827226877
2	10.326641816304944	10.501147894633885
3	3.261233678800899	4.974516080238646
4	1.4615492874510592	2.9724949302661323
5	0.821897881196227	2.0894670693187867
6	0.5047352247313001	1.5397936725662074
7	0.3759068819587241	1.3379071737912758
8	0.294160966834584	1.1965275343165793
9	0.21245326499437886	0.9721954515712535
>10	1.3927413959471735	13.315233444471966
>50	0.11631084719538266	4.119235137487479
>100	0.08204535177057756	7.755440556588529
>500	0.005352881989129492	1.8699533561967825
>1k	0.0029495472184999246	2.960953885585994
>5k	1.0924248957407129E-4	0.40119625413798365
>10k+	3.277274687222138E-4	2.737554836140754
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18839	1.0439854876097725	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16856	0.9340951950289467	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13591	0.7531613547483634	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7223	0.4002710959714097	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4016	0.22255139435431	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3847	0.21318605928312517	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3739	0.20720111142698336	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3565	0.19755869543653268	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3255	0.18037967844205158	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2918	0.161704424483535	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2058	0.11404650636981326	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2034	0.1127165179573373	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1999	0.11077695152247652	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1920	0.1063990729980765	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1878	0.10407159327624359	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1849	0.10246452394450181	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1840	0.10196577828982333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.108323677063297E-4	0.0
8	0.0	0.0	0.0	1.6624855155949453E-4	0.0
9	0.0	0.0	0.0	3.3249710311898905E-4	0.0
10	0.0	0.0	0.0	6.095780223848133E-4	0.0
11	1.108323677063297E-4	0.0	0.0	0.0012191560447696267	0.0
12	1.108323677063297E-4	0.0	0.0	0.0015516531478886157	0.0
13	1.108323677063297E-4	0.0	0.0	0.0015516531478886157	0.0
14	1.108323677063297E-4	0.0	0.0	0.0018287340671544398	0.0
15	1.108323677063297E-4	0.0	0.0	0.002549144457245583	0.0
16	1.108323677063297E-4	0.0	0.0	0.0034912195827493855	0.0
17	1.108323677063297E-4	0.0	0.0	0.004433294708253188	0.0
18	1.108323677063297E-4	0.0	0.0	0.005319953649903825	0.0
19	2.216647354126594E-4	0.0	0.0	0.006649942062379782	0.0
20	2.216647354126594E-4	0.0	0.0	0.010584491115954485	0.0
21	2.216647354126594E-4	0.0	0.0	0.017289849362187432	0.0
22	2.216647354126594E-4	0.0	0.0	0.03097764677391915	0.0
23	2.216647354126594E-4	0.0	0.0	0.03879132869721539	0.0
24	2.216647354126594E-4	0.0	0.0	0.051647883351149636	0.0
25	2.216647354126594E-4	0.0	0.0	0.06095780223848133	0.0
26	2.216647354126594E-4	0.0	0.0	0.07326019505388393	0.0
27	2.216647354126594E-4	0.0	0.0	0.12490807840503357	0.0
28	2.216647354126594E-4	0.0	0.0	0.24787659037520635	0.0
29	2.216647354126594E-4	0.0	0.0	0.4034298184510401	0.0
30	2.216647354126594E-4	0.0	0.0	0.6129584095998564	0.0
31	2.216647354126594E-4	0.0	0.0	0.9260044321863846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	65	2.6842554E-6	22.76923	14
GGTATCA	6250	0.0	20.6608	1
GTCGTAC	50	0.0070363996	18.499998	1
TTAACGG	210	0.0	17.619047	35
GTGCGCG	140	1.87174E-9	17.178572	11
TTAGAGT	145	2.9831426E-9	16.586206	4
TAACGGC	230	0.0	16.086956	36
CGTAATA	70	0.0025932607	15.857143	14
TTATGCG	240	0.0	15.416667	4
ACCGTTA	250	0.0	14.800001	8
CCGTTTA	290	0.0	14.672414	27
ATTGGAC	1010	0.0	14.10396	3
GTATCAA	9230	0.0	13.950163	2
CTAATAC	375	0.0	13.813333	3
TACCCCG	230	4.0017767E-11	13.673913	5
TCTATAC	150	1.3065619E-6	13.566667	3
TTGGACC	1315	0.0	13.505703	4
CCTATGT	360	0.0	13.361112	8
TAGCGAA	305	0.0	13.344262	10
CGCAATA	125	4.8473703E-5	13.320001	28
>>END_MODULE