FastQCFastQC Report
Wed 25 May 2016
SRR2088625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809823
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT438441.5603829849780573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT368631.3119331715912355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302381.0761531954148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157820.5616723900402267No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG85100.3028660524168248No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG84050.2991291622283681No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83870.2984885524817755No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82900.29503637773624886No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75960.2703373130620683No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG65530.23321753719006502No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA47330.16844477392348203No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45680.16257251791305002No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA44190.15726969278847813No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42830.15242953025866754No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC40390.14374570924930147No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39940.14214418488282002No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG38680.13765991665667196No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37950.1350618882399354No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36750.1307911565959849No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33970.12089729495416615No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC33040.11758747793010449No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC32520.11573682755105927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC909.490577E-820.5555577
GGTATCA139500.020.0648731
GTCGGGT2800.016.51785724
GTATTGG16700.016.2844311
TCGGTTC957.06548E-515.57894813
TTGGACC21750.015.140234
GTCTAAT1853.0559022E-1015.01
TAGCGAA4100.014.43902510
CGGGTAA2950.014.42372926
TAGTACT5700.014.2807034
GGTAGCG4150.014.265068
TTAACGG1304.449179E-614.23076935
CCGTTTA3650.014.19178127
TCGGGTA3000.014.18333325
ACGTTTA1702.4409019E-814.14705926
GGACCGT5800.014.0344836
ATTGGAC18500.013.93
AGTTCCG3600.013.87500132
ACCGTTA4000.013.8750018
TATTGGA19200.013.77864552
Produced by FastQC (version 0.11.5)