##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2809823
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676021229807002	31.0	31.0	34.0	30.0	34.0
2	31.83862257515865	31.0	31.0	34.0	30.0	34.0
3	31.91421203399645	31.0	31.0	34.0	30.0	34.0
4	35.594094361103885	37.0	35.0	37.0	33.0	37.0
5	35.51578480210319	37.0	35.0	37.0	33.0	37.0
6	35.5665278560251	37.0	35.0	37.0	33.0	37.0
7	35.415210851359674	37.0	35.0	37.0	33.0	37.0
8	35.46432070632207	37.0	35.0	37.0	33.0	37.0
9	37.0458164090763	39.0	37.0	39.0	33.0	39.0
10	36.92553445537317	39.0	37.0	39.0	33.0	39.0
11	36.96900552098833	39.0	37.0	39.0	33.0	39.0
12	36.87703140019852	39.0	37.0	39.0	32.0	39.0
13	36.92328769463415	39.0	37.0	39.0	33.0	39.0
14	37.89921180088568	40.0	37.0	41.0	33.0	41.0
15	37.87466470307916	40.0	37.0	41.0	32.0	41.0
16	37.77242054036856	40.0	37.0	41.0	32.0	41.0
17	37.84986954694299	40.0	37.0	41.0	32.0	41.0
18	37.8400564733081	40.0	37.0	41.0	32.0	41.0
19	37.878627586150444	40.0	37.0	41.0	32.0	41.0
20	37.773236250112554	40.0	37.0	41.0	32.0	41.0
21	37.68001614336561	40.0	37.0	41.0	32.0	41.0
22	37.53394003821593	39.0	36.0	41.0	32.0	41.0
23	37.38283479066119	39.0	36.0	41.0	31.0	41.0
24	37.23126581282878	39.0	36.0	41.0	31.0	41.0
25	37.04287850159957	39.0	36.0	41.0	31.0	41.0
26	37.16438615528451	39.0	36.0	41.0	31.0	41.0
27	37.08510144589179	39.0	36.0	41.0	31.0	41.0
28	36.94149382363231	39.0	35.0	41.0	31.0	41.0
29	36.774014590954664	39.0	35.0	41.0	30.0	41.0
30	36.62061951945016	39.0	35.0	40.0	30.0	41.0
31	36.389438765359955	38.0	35.0	40.0	30.0	41.0
32	36.25625315188893	38.0	35.0	40.0	30.0	41.0
33	36.02028561941446	38.0	35.0	40.0	30.0	41.0
34	35.85022472945805	38.0	35.0	40.0	29.0	41.0
35	35.63099383840192	38.0	35.0	40.0	27.0	41.0
36	35.464922879483865	38.0	35.0	40.0	27.0	41.0
37	35.27404501991762	38.0	34.0	40.0	26.0	41.0
38	35.13923190179595	38.0	34.0	40.0	25.0	41.0
39	34.95773256891982	38.0	34.0	40.0	24.0	41.0
40	34.74322012454165	38.0	33.0	40.0	23.0	41.0
41	34.57054554681914	38.0	33.0	40.0	22.0	41.0
42	34.294194687708085	38.0	33.0	40.0	21.0	41.0
43	33.912818351903304	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	7.0
14	4.0
15	15.0
16	38.0
17	71.0
18	220.0
19	518.0
20	1010.0
21	2021.0
22	3811.0
23	6588.0
24	10813.0
25	16615.0
26	24876.0
27	34818.0
28	47632.0
29	62060.0
30	77399.0
31	93727.0
32	114459.0
33	140893.0
34	174056.0
35	213615.0
36	276683.0
37	400638.0
38	511968.0
39	595266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.242909962656014	20.97484432293422	14.398380253845172	22.383865460564596
2	18.685269499181977	23.728576497523154	35.28827260649514	22.297881396799728
3	20.171519700707126	24.815228574895997	32.93702129991818	22.076230424478695
4	13.81830812830559	15.9870568359644	35.77791198947407	34.41672304625594
5	12.946651799775289	38.447475161246814	34.38177422563628	14.224098813341623
6	32.292140821681656	36.514684376916264	13.466471019704802	17.72670378169728
7	25.38273763151629	33.33758033869037	21.580434070046405	19.69924795974693
8	27.260151262196942	34.19991223646472	19.050345875879017	19.48959062545933
9	24.128993178573882	14.661848806846553	22.483444686729378	38.72571332785019
10	16.82664708773471	27.423862641881712	33.01496215242028	22.7345281179633
11	32.37178996684133	22.47031218692423	23.896095946257113	21.26180189997733
12	20.90370105163208	27.39770441056252	31.219724516455305	20.478870021350097
13	32.066646190881066	20.655073291093426	26.875287162216267	20.402993355809247
14	20.219494252840835	23.366560811837616	28.496670430842087	27.91727450447946
15	22.4814160891985	29.42971852675418	26.090362275488527	21.998503108558793
16	21.104425438897753	28.324880250464172	27.41023189005144	23.160462420586637
17	21.326254358370615	26.474870481165542	27.69971631665055	24.499158843813294
18	23.061132320434417	24.871139570001386	30.288064408327497	21.7796637012367
19	22.429491110294137	24.469406080027106	31.699114143488753	21.401988666190004
20	23.637076072051514	24.07799352485904	31.304925612752122	20.98000479033733
21	22.246027596756093	25.06976418087545	31.990164505023984	20.694043717344474
22	20.74682284257763	25.47519897160782	31.623486603960465	22.15449158185409
23	21.90219810998771	25.604495372128422	32.58603833764618	19.90726818023769
24	21.79870404648264	25.609904965544096	30.301695160157777	22.28969582781549
25	21.70474795031573	26.077087417961913	31.014551450393853	21.203613181328503
26	21.434161511241097	26.72698600588009	31.089324843593353	20.749527639285464
27	20.24750313453908	26.846495313049967	30.930631573590222	21.97536997882073
28	20.872951783795635	26.52028259431288	30.785818181429935	21.820947440461552
29	20.837611479441946	26.417251193402574	30.316713899772335	22.428423427383148
30	20.973669871732135	26.690898323488703	32.06077393487063	20.274657869908534
31	21.707381568162834	26.07989898296085	31.883147087912654	20.329572360963663
32	21.422025515486208	26.04434514202496	30.585734403910852	21.94789493857798
33	20.932492900798376	26.16816077026916	31.242394983598608	21.65695134533385
34	20.145397058818297	26.7861356391488	31.70285103367721	21.365616268355694
35	19.67106113089686	28.945488737190917	30.697129320957227	20.686320810954996
36	21.8947243296108	26.178730831087936	30.78991096592205	21.136633873379214
37	20.98281635533626	26.99533031084164	30.57263749353607	21.449215840286026
38	20.73806784270753	25.37601122917707	32.037178142537805	21.848742785577596
39	20.149454253880048	25.202619524432677	32.87990026418034	21.768025957506932
40	19.004542278997643	25.972418903254763	32.41531584017926	22.607722977568336
41	20.575175019921183	24.9878729016027	32.27633911459903	22.160612963877085
42	19.159676605964147	27.07074431378774	31.620532681240064	22.14904639900805
43	19.35417284291573	27.24328187220334	30.654671130530286	22.747874154350647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159.0
1	415.0
2	671.0
3	1970.0
4	3269.0
5	3269.0
6	5135.5
7	7002.0
8	7664.0
9	8326.0
10	12145.5
11	15965.0
12	15965.0
13	28811.5
14	41658.0
15	65439.0
16	89220.0
17	80641.0
18	72062.0
19	72062.0
20	76466.5
21	80871.0
22	62401.0
23	43931.0
24	48245.0
25	52559.0
26	52559.0
27	56125.5
28	59692.0
29	63146.5
30	66601.0
31	70911.0
32	75221.0
33	75221.0
34	81376.0
35	87531.0
36	97197.0
37	106863.0
38	112972.0
39	119081.0
40	119081.0
41	125094.0
42	131107.0
43	140636.0
44	150165.0
45	167883.0
46	185601.0
47	185601.0
48	250202.0
49	314803.0
50	293773.0
51	272743.0
52	249392.0
53	226041.0
54	226041.0
55	198451.0
56	170861.0
57	143818.5
58	116776.0
59	102628.5
60	88481.0
61	88481.0
62	77666.0
63	66851.0
64	57922.0
65	48993.0
66	42463.0
67	35933.0
68	35933.0
69	30958.0
70	25983.0
71	21913.5
72	17844.0
73	14734.0
74	11624.0
75	11624.0
76	9219.5
77	6815.0
78	5354.0
79	3893.0
80	3040.5
81	2188.0
82	2188.0
83	1677.5
84	1167.0
85	876.5
86	586.0
87	465.0
88	344.0
89	344.0
90	288.0
91	232.0
92	153.0
93	74.0
94	47.5
95	21.0
96	21.0
97	16.5
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2809823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.82187055085697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09510383590816	35.0036863478881
2	11.683405237370495	10.473441542852493
3	4.096078596886118	5.507817139072963
4	1.8484619777492797	3.314060939394371
5	0.9850314912540956	2.207547699475434
6	0.6227913084883652	1.67488028455586
7	0.41454189195095187	1.3006380113253033
8	0.3152621017455686	1.1304509691224782
9	0.22726133490104306	0.9167650320734555
>10	1.4568944156347765	12.460094294279395
>50	0.14439694929089608	4.505550444592852
>100	0.09758277781831545	8.253206955511779
>500	0.006991271856539575	2.1113112902206996
>1k	0.005402346434597669	4.927864861778776
>5k	4.7667762658214723E-4	1.7000238199121342
>10k+	3.177850843880982E-4	4.512660367943802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43844	1.5603829849780573	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36863	1.3119331715912355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30238	1.0761531954148	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15782	0.5616723900402267	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8510	0.3028660524168248	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8405	0.2991291622283681	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8387	0.2984885524817755	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8290	0.29503637773624886	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7596	0.2703373130620683	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6553	0.23321753719006502	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4733	0.16844477392348203	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4568	0.16257251791305002	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4419	0.15726969278847813	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4283	0.15242953025866754	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4039	0.14374570924930147	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3994	0.14214418488282002	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3868	0.13765991665667196	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3795	0.1350618882399354	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3675	0.1307911565959849	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3397	0.12089729495416615	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3304	0.11758747793010449	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3252	0.11573682755105927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.558943036625439E-5	0.0
2	0.0	0.0	0.0	3.558943036625439E-5	0.0
3	0.0	0.0	0.0	3.558943036625439E-5	0.0
4	0.0	0.0	0.0	3.558943036625439E-5	0.0
5	0.0	0.0	0.0	3.558943036625439E-5	0.0
6	3.558943036625439E-5	0.0	0.0	3.558943036625439E-5	0.0
7	7.117886073250877E-5	0.0	0.0	3.558943036625439E-5	0.0
8	1.0676829109876315E-4	0.0	0.0	7.117886073250877E-5	0.0
9	1.0676829109876315E-4	0.0	0.0	7.117886073250877E-5	0.0
10	1.7794715183127194E-4	0.0	0.0	2.847154429300351E-4	0.0
11	1.7794715183127194E-4	0.0	0.0	7.117886073250878E-4	0.0
12	1.7794715183127194E-4	0.0	0.0	0.0014235772146501755	0.0
13	1.7794715183127194E-4	0.0	0.0	0.0017082926575802105	0.0
14	2.491260125637807E-4	0.0	0.0	0.0019218292397777368	0.0
15	2.491260125637807E-4	0.0	0.0	0.0026336178471028245	0.0
16	2.491260125637807E-4	0.0	0.0	0.0036301218973579475	0.0
17	2.847154429300351E-4	0.0	0.0	0.005018109681641869	0.0
18	3.203048732962895E-4	0.0	0.0	0.005623129997868193	0.0
19	3.914837340287983E-4	0.0	0.0	0.007295833225082149	0.0
20	3.914837340287983E-4	0.0	0.0	0.012990142083682852	0.0
21	3.914837340287983E-4	3.558943036625439E-5	0.0	0.023382255750629133	0.0
22	3.914837340287983E-4	3.558943036625439E-5	0.0	0.03722654416310209	0.0
23	3.914837340287983E-4	3.558943036625439E-5	0.0	0.04712040580492081	0.0
24	4.270731643950526E-4	3.558943036625439E-5	0.0	0.061961198267648884	0.0
25	4.270731643950526E-4	3.558943036625439E-5	0.0	0.07267361680789146	0.0
26	4.62662594761307E-4	3.558943036625439E-5	0.0	0.0876211775617183	0.0
27	4.62662594761307E-4	3.558943036625439E-5	0.0	0.15221599367647	0.0
28	4.62662594761307E-4	3.558943036625439E-5	0.0	0.3014068857718084	0.0
29	4.62662594761307E-4	3.558943036625439E-5	0.0	0.5021668624678494	0.0
30	4.62662594761307E-4	3.558943036625439E-5	0.0	0.7856722647654318	0.0
31	4.982520251275614E-4	3.558943036625439E-5	0.0	1.1884022587899665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTC	90	9.490577E-8	20.555557	7
GGTATCA	13950	0.0	20.064873	1
GTCGGGT	280	0.0	16.517857	24
GTATTGG	1670	0.0	16.284431	1
TCGGTTC	95	7.06548E-5	15.578948	13
TTGGACC	2175	0.0	15.14023	4
GTCTAAT	185	3.0559022E-10	15.0	1
TAGCGAA	410	0.0	14.439025	10
CGGGTAA	295	0.0	14.423729	26
TAGTACT	570	0.0	14.280703	4
GGTAGCG	415	0.0	14.26506	8
TTAACGG	130	4.449179E-6	14.230769	35
CCGTTTA	365	0.0	14.191781	27
TCGGGTA	300	0.0	14.183333	25
ACGTTTA	170	2.4409019E-8	14.147059	26
GGACCGT	580	0.0	14.034483	6
ATTGGAC	1850	0.0	13.9	3
AGTTCCG	360	0.0	13.875001	32
ACCGTTA	400	0.0	13.875001	8
TATTGGA	1920	0.0	13.7786455	2
>>END_MODULE