FastQCFastQC Report
Wed 25 May 2016
SRR2088624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957692
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT468471.1836949413951363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT391640.9895666464191756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319160.8064296059420489No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171920.43439459159530347No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA82510.2084800939537488No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81560.2060797050402103No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG79320.20041984065460372No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA73440.1855626966423865No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA68530.1731564760471507No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61500.15539359808696582No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48210.12181342054914834No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44060.11132751108474334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.10677940577488092No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41850.10574344845430116No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40810.10311565427526953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140650.020.9399221
ACGTTTA1850.019.026
GTATTGG16650.017.01
ACCGTTA7200.016.701398
CCGTTTA7450.015.64429527
TAATACT10150.015.12807754
GACCGTT7950.014.8930817
TGTTACG4600.014.88043516
GGACCGT10100.014.6534646
CTAATAC11250.014.6355563
TCTAATA11000.014.1272722
ATTGGAC21250.013.9294113
CGTTTAC8450.013.792928
GTATCAA214100.013.7389072
CTCTAAT10100.013.7376231
TACTCCG9200.013.6739135
CCGTTAT8700.013.6091959
TGGACCC22500.013.4022235
CGCCGTT8650.013.26011525
GTATACT2102.046363E-913.2142854
Produced by FastQC (version 0.11.5)