##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3957692
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.650564773610476	31.0	31.0	34.0	30.0	34.0
2	31.826678023454075	31.0	31.0	34.0	30.0	34.0
3	31.902117951573796	31.0	31.0	34.0	30.0	34.0
4	35.59634099874371	37.0	35.0	37.0	33.0	37.0
5	35.50056901850877	37.0	35.0	37.0	33.0	37.0
6	35.538698059374	37.0	35.0	37.0	33.0	37.0
7	35.40771591119268	37.0	35.0	37.0	33.0	37.0
8	35.434840811260706	37.0	35.0	37.0	33.0	37.0
9	37.0310200995934	39.0	37.0	39.0	33.0	39.0
10	36.883570272775145	39.0	37.0	39.0	33.0	39.0
11	36.92959027635298	39.0	37.0	39.0	33.0	39.0
12	36.833805915164696	39.0	37.0	39.0	32.0	39.0
13	36.88371909688778	39.0	37.0	39.0	33.0	39.0
14	37.897323743232164	40.0	37.0	41.0	33.0	41.0
15	37.85528787990577	40.0	37.0	41.0	32.0	41.0
16	37.75416303239363	40.0	37.0	41.0	32.0	41.0
17	37.788047932987205	40.0	37.0	41.0	32.0	41.0
18	37.765006220797375	40.0	37.0	41.0	32.0	41.0
19	37.790734852535266	40.0	37.0	41.0	32.0	41.0
20	37.67432736049192	40.0	37.0	41.0	32.0	41.0
21	37.57405326134525	39.0	37.0	41.0	32.0	41.0
22	37.419528604044984	39.0	36.0	41.0	32.0	41.0
23	37.28877689319937	39.0	36.0	41.0	31.0	41.0
24	37.14933931190199	39.0	36.0	41.0	31.0	41.0
25	36.941075505622976	39.0	36.0	41.0	30.0	41.0
26	37.08986222272981	39.0	36.0	41.0	31.0	41.0
27	37.01024864997074	39.0	36.0	41.0	31.0	41.0
28	36.88759458795682	39.0	35.0	41.0	30.0	41.0
29	36.74037949390706	39.0	35.0	41.0	30.0	41.0
30	36.61490282720333	39.0	35.0	40.0	30.0	41.0
31	36.42984598094041	39.0	35.0	40.0	30.0	41.0
32	36.30331112173459	38.0	35.0	40.0	30.0	41.0
33	36.10057553745971	38.0	35.0	40.0	30.0	41.0
34	35.958697392318555	38.0	35.0	40.0	29.0	41.0
35	35.77042579361911	38.0	35.0	40.0	28.0	41.0
36	35.62511332362397	38.0	35.0	40.0	27.0	41.0
37	35.45893060905194	38.0	34.0	40.0	26.0	41.0
38	35.367122302594545	38.0	34.0	40.0	26.0	41.0
39	35.22370361311593	38.0	34.0	40.0	25.0	41.0
40	35.0349875634587	38.0	34.0	40.0	24.0	41.0
41	34.900813403367415	38.0	34.0	40.0	24.0	41.0
42	34.68220619492371	38.0	33.0	40.0	23.0	41.0
43	34.31158791538098	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	5.0
13	5.0
14	13.0
15	33.0
16	53.0
17	142.0
18	337.0
19	735.0
20	1511.0
21	3011.0
22	5744.0
23	9504.0
24	15285.0
25	23554.0
26	34269.0
27	47841.0
28	64634.0
29	84389.0
30	105719.0
31	130496.0
32	159770.0
33	196763.0
34	243091.0
35	300137.0
36	391130.0
37	554229.0
38	754417.0
39	830869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77027924355913	21.114781039050033	13.797587078529608	23.317352638861237
2	19.182670101665313	23.192431346350347	34.720008530224185	22.904890021760156
3	20.090749861282788	24.61053058196545	32.110861582963004	23.187857973788763
4	13.96955599374585	16.35574471181689	35.9862263157416	33.688472978695664
5	13.19812153143802	38.23147430371034	34.590033787369	13.980370377482634
6	32.60796949333096	36.97773854054333	13.812671627807319	16.601620338318394
7	26.409659973540133	32.51185286778254	21.63313870811574	19.44534845056159
8	27.066911725318697	34.36636807513065	18.979066587293804	19.58765361225684
9	24.77267053626204	14.40268722275508	21.642209651483743	39.182432589499136
10	16.869276335803796	27.178870917696475	33.236290241888454	22.715562504611274
11	33.424581801716755	22.481107675887866	23.234551854970018	20.859758667425357
12	21.17193556244397	26.987976830940863	30.70870598318414	21.131381623431032
13	31.291469876887845	20.734458366138647	26.125959271211606	21.848112485761902
14	20.66254776773938	22.731986218230222	27.6386085627684	28.966857451261994
15	23.389616978784606	29.13104405294803	25.20216328102338	22.27717568724398
16	22.1953603261699	27.860177093113865	26.55055016913898	23.393912411577254
17	22.065284514307834	26.49059098080396	27.097106091125838	24.347018413762363
18	22.80937981025305	25.35452480890378	29.41039878798047	22.42569659286271
19	23.057706360171533	24.818606399891653	30.243990689523088	21.879696550413726
20	23.72769280681771	24.558682181433017	30.04316657284094	21.670458438908334
21	22.729813234582174	25.361043759847913	30.362241427579505	21.546901577990404
22	21.82913677971909	25.811634659796667	29.96450961823204	22.3947189422522
23	22.38948862114586	25.917327573747528	30.710373621797753	20.98281018330886
24	22.475852087529802	26.09167161062559	28.627821467663477	22.804654834181136
25	22.517164044094386	25.934660908428448	29.606826402863085	21.941348644614084
26	22.18426800266418	26.62377466462777	29.674087826945605	21.517869505762448
27	21.542050265659885	26.616068152852723	29.248789445970026	22.593092135517367
28	21.748104703448373	26.367236257899805	29.538554288711705	22.346104749940114
29	22.085548850188445	26.36468426547594	29.03616552273396	22.51360136160166
30	21.862716957256907	26.51080478218113	30.42571276390381	21.200765496658153
31	22.316087254895027	26.17373964421688	30.21493335004341	21.295239750844686
32	21.90281608573886	26.039039925289792	29.434402677115855	22.623741311855493
33	21.68966155021664	26.11443740442662	29.897677737428786	22.29822330792795
34	21.224845187548702	26.383912644035966	30.36901305104086	22.02222911737447
35	20.98558958099822	27.96377282517184	29.438394902887843	21.612242690942093
36	22.551729644449342	26.099403389652352	29.482789464162444	21.86607750173586
37	21.6348568812328	26.42572994563498	29.48195564485564	22.45745752827658
38	21.53411634861935	25.262501478134226	30.699660307067855	22.503721866178573
39	21.022126026987443	25.2426919527846	31.315574835030112	22.419607185197837
40	20.282048224065946	25.910808622803394	30.96544652792587	22.84169662520479
41	21.179414668953523	24.868686092803582	31.120183177468082	22.831716060774816
42	20.022225074614195	26.586859209862716	30.5600587413068	22.830856974216285
43	20.34850109609338	26.210175021199223	30.077580569685562	23.363743313021832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	477.0
2	749.0
3	2113.0
4	3477.0
5	3477.0
6	5365.5
7	7254.0
8	7991.0
9	8728.0
10	12847.0
11	16966.0
12	16966.0
13	31033.5
14	45101.0
15	69999.5
16	94898.0
17	86359.5
18	77821.0
19	77821.0
20	83030.0
21	88239.0
22	70038.5
23	51838.0
24	57931.0
25	64024.0
26	64024.0
27	70468.0
28	76912.0
29	85218.0
30	93524.0
31	103195.5
32	112867.0
33	112867.0
34	125982.5
35	139098.0
36	154225.0
37	169352.0
38	182573.5
39	195795.0
40	195795.0
41	202899.0
42	210003.0
43	226934.5
44	243866.0
45	254880.0
46	265894.0
47	265894.0
48	326190.0
49	386486.0
50	370146.0
51	353806.0
52	329454.5
53	305103.0
54	305103.0
55	275804.0
56	246505.0
57	214343.0
58	182181.0
59	162225.0
60	142269.0
61	142269.0
62	126526.5
63	110784.0
64	96774.5
65	82765.0
66	71390.5
67	60016.0
68	60016.0
69	51676.0
70	43336.0
71	37018.0
72	30700.0
73	25259.5
74	19819.0
75	19819.0
76	15947.5
77	12076.0
78	9442.0
79	6808.0
80	5360.5
81	3913.0
82	3913.0
83	2996.5
84	2080.0
85	1616.5
86	1153.0
87	882.0
88	611.0
89	611.0
90	497.5
91	384.0
92	282.5
93	181.0
94	125.0
95	69.0
96	69.0
97	47.0
98	25.0
99	18.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3957692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.20001897841226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.34192683726563	32.97972688218435
2	12.370058376493892	10.687735132572072
3	4.528666187113601	5.869153956906045
4	2.0407341578220715	3.526390173912306
5	1.1627338677478511	2.511506257677493
6	0.7028405653300857	1.8217635456634618
7	0.4546779076268452	1.374946596698114
8	0.33100911041202447	1.143967988146145
9	0.23836792070043175	0.9267748828292988
>10	1.5382713075743082	12.732063650621905
>50	0.159486551640444	4.798699100428106
>100	0.1154624484420603	9.89020201019907
>500	0.00931820495522232	2.7453933390629857
>1k	0.005860506250505256	4.439481713324233
>5k	3.5163037503031536E-4	1.1313332525910973
>10k+	2.344202500202102E-4	3.4208615171833787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	46847	1.1836949413951363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39164	0.9895666464191756	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31916	0.8064296059420489	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17192	0.43439459159530347	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8251	0.2084800939537488	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8156	0.2060797050402103	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7932	0.20041984065460372	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7344	0.1855626966423865	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6853	0.1731564760471507	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6150	0.15539359808696582	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4821	0.12181342054914834	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4406	0.11132751108474334	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4226	0.10677940577488092	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4185	0.10574344845430116	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4081	0.10311565427526953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.526725172145786E-5	0.0
5	0.0	0.0	0.0	2.526725172145786E-5	0.0
6	7.580175516437358E-5	0.0	0.0	5.053450344291572E-5	0.0
7	7.580175516437358E-5	0.0	0.0	5.053450344291572E-5	0.0
8	7.580175516437358E-5	0.0	0.0	5.053450344291572E-5	0.0
9	7.580175516437358E-5	0.0	0.0	1.5160351032874716E-4	0.0
10	7.580175516437358E-5	0.0	0.0	1.76870762050205E-4	0.0
11	7.580175516437358E-5	0.0	0.0	4.295432792647836E-4	0.0
12	7.580175516437358E-5	0.0	0.0	5.55879537872073E-4	0.0
13	7.580175516437358E-5	0.0	0.0	6.316812930364465E-4	0.0
14	7.580175516437358E-5	0.0	0.0	7.580175516437358E-4	0.0
15	7.580175516437358E-5	0.0	0.0	0.0010106900688583143	0.0
16	1.0106900688583144E-4	0.0	0.0	0.0015665696067303874	0.0
17	1.0106900688583144E-4	0.0	0.0	0.00247619066870287	0.0
18	1.263362586072893E-4	0.0	0.0	0.0029310011996891115	0.0
19	1.263362586072893E-4	0.0	0.0	0.0038658895133830526	0.0
20	1.263362586072893E-4	0.0	0.0	0.00636734743380738	0.0
21	1.263362586072893E-4	0.0	0.0	0.010940719995391254	0.0
22	1.76870762050205E-4	0.0	0.0	0.01841982650494278	0.0
23	1.76870762050205E-4	0.0	0.0	0.02382701837333476	0.0
24	1.76870762050205E-4	0.0	0.0	0.0327968927344523	0.0
25	1.76870762050205E-4	0.0	0.0	0.03898736940620948	0.0
26	1.76870762050205E-4	0.0	0.0	0.04863945956380638	0.0
27	2.0213801377166288E-4	0.0	0.0	0.10261030924084037	0.0
28	2.0213801377166288E-4	0.0	0.0	0.22295822919014416	0.0
29	2.2740526549312073E-4	0.0	0.0	0.3791098448287537	0.0
30	2.2740526549312073E-4	0.0	0.0	0.5967872184091132	0.0
31	2.2740526549312073E-4	0.0	0.0	0.9171254357337559	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14065	0.0	20.939922	1
ACGTTTA	185	0.0	19.0	26
GTATTGG	1665	0.0	17.0	1
ACCGTTA	720	0.0	16.70139	8
CCGTTTA	745	0.0	15.644295	27
TAATACT	1015	0.0	15.1280775	4
GACCGTT	795	0.0	14.893081	7
TGTTACG	460	0.0	14.880435	16
GGACCGT	1010	0.0	14.653464	6
CTAATAC	1125	0.0	14.635556	3
TCTAATA	1100	0.0	14.127272	2
ATTGGAC	2125	0.0	13.929411	3
CGTTTAC	845	0.0	13.7929	28
GTATCAA	21410	0.0	13.738907	2
CTCTAAT	1010	0.0	13.737623	1
TACTCCG	920	0.0	13.673913	5
CCGTTAT	870	0.0	13.609195	9
TGGACCC	2250	0.0	13.402223	5
CGCCGTT	865	0.0	13.260115	25
GTATACT	210	2.046363E-9	13.214285	4
>>END_MODULE