FastQCFastQC Report
Wed 25 May 2016
SRR2088623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575955
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT367321.0271941341543727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT313500.8766888845077748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255550.7146342725230044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135800.37975869383143807No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG67090.18761421774043577No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63390.17726733138420367No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG62790.1755894579210309No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA61540.17209388820608762No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA57150.15981744736720682No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG49940.13965500125141395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.10352479267776021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.020.4418091
GACCGTT6600.017.3787887
TAGACCG1401.873559E-917.1785725
ACGTTTA1851.8189894E-1217.026
GTATTGG15500.016.3516141
ACCGTTA7100.015.6338028
TTATGCG4200.015.4166684
TAGTACT6700.015.1865674
GGACCGT8950.014.8826826
TGTTACG4250.014.79999916
TCTACAC4700.014.5638283
GCTTATG6100.014.5573762
ATTGGAC19150.014.2976513
CCGTTTA7400.014.2527
TTGGACC23850.014.11744
GTATTAG7450.013.906041
TAGGTCG1907.1413524E-913.63157921
GTATCAA172150.013.5082782
CGTTCGC6300.013.507936523
CGTTTAC7900.013.34810128
Produced by FastQC (version 0.11.5)