##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3575955
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.709583034462124	31.0	31.0	34.0	30.0	34.0
2	31.888404076673226	31.0	31.0	34.0	30.0	34.0
3	31.968886633081233	31.0	31.0	34.0	30.0	34.0
4	35.654853598549195	37.0	35.0	37.0	33.0	37.0
5	35.55868096774149	37.0	35.0	37.0	33.0	37.0
6	35.5859984255954	37.0	35.0	37.0	33.0	37.0
7	35.45524873774978	37.0	35.0	37.0	33.0	37.0
8	35.47657842450478	37.0	35.0	37.0	33.0	37.0
9	37.08820776547803	39.0	37.0	39.0	33.0	39.0
10	36.94470875612249	39.0	37.0	39.0	33.0	39.0
11	36.989495673183804	39.0	37.0	39.0	33.0	39.0
12	36.89564857499605	39.0	37.0	39.0	33.0	39.0
13	36.93943967415697	39.0	37.0	39.0	33.0	39.0
14	37.98433733086686	40.0	37.0	41.0	33.0	41.0
15	37.94194278171845	40.0	37.0	41.0	33.0	41.0
16	37.84414373223377	40.0	37.0	41.0	32.0	41.0
17	37.86316634297691	40.0	37.0	41.0	33.0	41.0
18	37.83761876198106	40.0	37.0	41.0	32.0	41.0
19	37.85569756890118	40.0	37.0	41.0	32.0	41.0
20	37.73343344644997	40.0	37.0	41.0	32.0	41.0
21	37.63481671329757	39.0	37.0	41.0	32.0	41.0
22	37.47785500656468	39.0	36.0	41.0	32.0	41.0
23	37.353916646042805	39.0	36.0	41.0	31.0	41.0
24	37.2155810126246	39.0	36.0	41.0	31.0	41.0
25	37.01348478937794	39.0	36.0	41.0	31.0	41.0
26	37.170307512258965	39.0	36.0	41.0	31.0	41.0
27	37.098446149350316	39.0	36.0	41.0	31.0	41.0
28	36.979343420149306	39.0	36.0	41.0	31.0	41.0
29	36.836580717598515	39.0	35.0	41.0	30.0	41.0
30	36.71558870287797	39.0	35.0	41.0	30.0	41.0
31	36.543325908743256	39.0	35.0	40.0	30.0	41.0
32	36.420900990085165	39.0	35.0	40.0	30.0	41.0
33	36.2272232172944	38.0	35.0	40.0	30.0	41.0
34	36.090708356229314	38.0	35.0	40.0	29.0	41.0
35	35.90951871597937	38.0	35.0	40.0	28.0	41.0
36	35.7731238228669	38.0	35.0	40.0	27.0	41.0
37	35.6077990355024	38.0	35.0	40.0	27.0	41.0
38	35.523842721734475	38.0	35.0	40.0	26.0	41.0
39	35.39217439816776	38.0	34.0	40.0	26.0	41.0
40	35.22613735351815	38.0	34.0	40.0	25.0	41.0
41	35.09962905014185	38.0	34.0	40.0	24.0	41.0
42	34.895085089158	38.0	34.0	40.0	24.0	41.0
43	34.53972854803822	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	8.0
14	9.0
15	16.0
16	47.0
17	114.0
18	245.0
19	589.0
20	1251.0
21	2498.0
22	4671.0
23	8244.0
24	13075.0
25	20007.0
26	29132.0
27	40665.0
28	55156.0
29	73001.0
30	92436.0
31	114721.0
32	141159.0
33	173498.0
34	216301.0
35	268254.0
36	347110.0
37	493246.0
38	679265.0
39	801230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0717822232103	20.731748581847366	13.484565661480637	23.71190353346169
2	19.097527793274804	22.76905050538947	34.71808230248982	23.4153393988459
3	19.96476465727337	24.44096751776798	31.94562012105857	23.648647703900078
4	13.982111072426806	16.105851443880027	36.13197034079008	33.780067142903086
5	13.277488111567399	38.06745330967532	34.73251201427311	13.922546564484172
6	32.882069265413016	36.874932710282984	13.89379899914848	16.349199025155517
7	26.608276670148257	32.291010373452686	21.709053945030067	19.391659011368994
8	27.088791665443217	34.354850662270636	19.06321528095292	19.493142391333222
9	24.936387622327462	14.412933048654136	21.383154989366478	39.26752433965193
10	16.80798555910239	27.02511636751581	33.38042564853305	22.786472424848746
11	33.72525660977278	22.350309218096985	23.076213207380967	20.848220964749274
12	21.042071278861172	26.724721088492444	30.777037183074174	21.456170449572213
13	31.25679713531071	20.676993977832495	25.916125902031766	22.150082984825033
14	20.86211934993589	22.487363515480478	27.277468536376997	29.37304859820663
15	23.617746867619978	29.034481697896087	24.82355063192909	22.524220802554844
16	22.509763126213837	27.76978457502961	26.157711716170923	23.562740582585633
17	22.194378844252793	26.57094398559266	26.90017072362488	24.33450644652967
18	23.053422092839533	25.475264649583117	28.84502181934616	22.626291438231185
19	23.269112726530395	24.960688822985748	29.668801760648556	22.101396689835305
20	24.00642625536395	24.644941001774352	29.36340082579339	21.985231917068308
21	22.970227533623884	25.422327741820016	29.720983625353227	21.88646109920287
22	22.192533183443302	25.758601548397557	29.3838708820441	22.664994386115037
23	22.644943798230123	25.939811882420223	30.07462342227461	21.34062089707505
24	22.796483736512343	26.03290589506859	28.140510716717632	23.030099651701434
25	22.857530366014114	25.909722018313992	29.011187221315705	22.221560394356192
26	22.52052948093586	26.58679988981964	29.056629627609965	21.83604100163453
27	21.903351692065478	26.597985712907462	28.72849350732881	22.77016908769825
28	22.035819801982967	26.348737609953144	28.98610301304127	22.629339575022616
29	22.379056783432677	26.384588172949602	28.50058236191451	22.73577268170321
30	22.20492148251306	26.484673325027856	29.817573207716542	21.492831984742537
31	22.55942818072375	26.10955674777787	29.716201685983183	21.6148133855152
32	22.191246813788204	26.058996827420927	28.910682600871656	22.839073757919213
33	22.000388707352304	26.043280745982543	29.398132806481065	22.55819774018409
34	21.62457301615932	26.25298696432142	29.781582821931487	22.340857197587777
35	21.355553970897283	27.73312303985928	28.973854536760108	21.937468452483323
36	22.834068102087414	25.981339250633745	28.98506832440565	22.19952432287319
37	21.97829670675386	26.28495045379486	28.97307152914396	22.763681310307316
38	21.81744457075103	25.235049098772215	30.227253978307893	22.72025235216886
39	21.284160455039284	25.23538467346485	30.798849538095418	22.681605333400448
40	20.704818712763444	25.775016743778934	30.528376335831968	22.991788207625653
41	21.43217685904884	24.758225425096235	30.666241605389327	23.143356110465596
42	20.368209331493265	26.33181905253282	30.184188559419788	23.115783056554122
43	20.663431167338516	25.948872399121353	29.749675261573483	23.63802117196665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	393.0
2	604.0
3	1754.0
4	2904.0
5	2904.0
6	4505.0
7	6106.0
8	6662.5
9	7219.0
10	10488.5
11	13758.0
12	13758.0
13	24739.0
14	35720.0
15	55650.5
16	75581.0
17	69517.5
18	63454.0
19	63454.0
20	67770.0
21	72086.0
22	58059.5
23	44033.0
24	50045.0
25	56057.0
26	56057.0
27	62329.5
28	68602.0
29	75941.5
30	83281.0
31	92091.0
32	100901.0
33	100901.0
34	113978.0
35	127055.0
36	140031.0
37	153007.0
38	164649.0
39	176291.0
40	176291.0
41	184551.5
42	192812.0
43	207575.0
44	222338.0
45	232995.5
46	243653.0
47	243653.0
48	294787.5
49	345922.0
50	331618.0
51	317314.0
52	297965.5
53	278617.0
54	278617.0
55	252551.5
56	226486.0
57	199321.0
58	172156.0
59	154250.0
60	136344.0
61	136344.0
62	120800.5
63	105257.0
64	91944.0
65	78631.0
66	67618.0
67	56605.0
68	56605.0
69	48741.5
70	40878.0
71	34766.0
72	28654.0
73	23710.5
74	18767.0
75	18767.0
76	14858.0
77	10949.0
78	8613.5
79	6278.0
80	4962.5
81	3647.0
82	3647.0
83	2688.0
84	1729.0
85	1376.0
86	1023.0
87	769.5
88	516.0
89	516.0
90	425.0
91	334.0
92	232.5
93	131.0
94	86.0
95	41.0
96	41.0
97	32.0
98	23.0
99	16.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3575955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.868295867237144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60404186570837	35.595651527897694
2	12.123853862505014	11.122010320330515
3	4.06587082459002	5.594836978207874
4	1.8771574399979514	3.44408051388846
5	1.0222697551125588	2.344488579181546
6	0.6293929832865881	1.7321510144491359
7	0.4346536486712838	1.3955775509920019
8	0.307138283336907	1.1270327721802051
9	0.2374841011119785	0.9803691912212182
>10	1.4314751809016573	12.50785873018664
>50	0.1470629010717443	4.708900979558641
>100	0.10653542977385475	9.57731270468897
>500	0.008424270574153516	2.6994724493920894
>1k	0.0040900444076906245	3.2946231300846542
>5k	3.0522719460377795E-4	0.8735036771637718
>10k+	2.441817556830224E-4	3.002129880576616
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36732	1.0271941341543727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31350	0.8766888845077748	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25555	0.7146342725230044	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13580	0.37975869383143807	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6709	0.18761421774043577	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6339	0.17726733138420367	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6279	0.1755894579210309	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6154	0.17209388820608762	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5715	0.15981744736720682	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4994	0.13965500125141395	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3702	0.10352479267776021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.7964557719546248E-5
3	0.0	0.0	0.0	0.0	2.7964557719546248E-5
4	2.7964557719546248E-5	0.0	0.0	0.0	2.7964557719546248E-5
5	2.7964557719546248E-5	0.0	0.0	0.0	2.7964557719546248E-5
6	5.5929115439092495E-5	0.0	0.0	0.0	2.7964557719546248E-5
7	5.5929115439092495E-5	0.0	0.0	0.0	2.7964557719546248E-5
8	1.1185823087818499E-4	0.0	0.0	0.0	2.7964557719546248E-5
9	1.1185823087818499E-4	0.0	0.0	1.6778734631727748E-4	2.7964557719546248E-5
10	1.3982278859773123E-4	0.0	0.0	2.2371646175636998E-4	2.7964557719546248E-5
11	1.6778734631727748E-4	0.0	0.0	5.313265966713787E-4	2.7964557719546248E-5
12	1.9575190403682373E-4	0.0	0.0	9.507949624645724E-4	2.7964557719546248E-5
13	2.2371646175636998E-4	0.0	0.0	0.0010346886356232112	2.7964557719546248E-5
14	2.5168101947591623E-4	0.0	0.0	0.0012863696550991274	2.7964557719546248E-5
15	2.5168101947591623E-4	0.0	0.0	0.0015380506745750436	2.7964557719546248E-5
16	2.5168101947591623E-4	0.0	0.0	0.00201344815580733	2.7964557719546248E-5
17	2.5168101947591623E-4	0.0	0.0	0.0023210582907223385	2.7964557719546248E-5
18	3.0761013491500873E-4	0.0	0.0	0.002936278560552356	2.7964557719546248E-5
19	3.0761013491500873E-4	0.0	0.0	0.003915038080736475	2.7964557719546248E-5
20	3.3557469263455496E-4	0.0	0.0	0.006068309025141536	2.7964557719546248E-5
21	3.3557469263455496E-4	0.0	0.0	0.010179099009914833	2.7964557719546248E-5
22	3.3557469263455496E-4	0.0	0.0	0.01661094728541047	2.7964557719546248E-5
23	3.3557469263455496E-4	0.0	2.7964557719546248E-5	0.02175642590580698	2.7964557719546248E-5
24	3.3557469263455496E-4	0.0	5.5929115439092495E-5	0.029390750163243107	2.7964557719546248E-5
25	3.3557469263455496E-4	0.0	5.5929115439092495E-5	0.03445233511048098	2.7964557719546248E-5
26	3.3557469263455496E-4	0.0	5.5929115439092495E-5	0.04365267460021169	2.7964557719546248E-5
27	3.6353925035410124E-4	0.0	5.5929115439092495E-5	0.09740055453717958	2.7964557719546248E-5
28	3.6353925035410124E-4	0.0	5.5929115439092495E-5	0.21297807159206422	2.7964557719546248E-5
29	3.6353925035410124E-4	0.0	5.5929115439092495E-5	0.36591623776026266	2.7964557719546248E-5
30	4.4743292351273996E-4	0.0	5.5929115439092495E-5	0.5656111444355424	2.7964557719546248E-5
31	4.4743292351273996E-4	0.0	5.5929115439092495E-5	0.8632379322446732	2.7964557719546248E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11385	0.0	20.441809	1
GACCGTT	660	0.0	17.378788	7
TAGACCG	140	1.873559E-9	17.178572	5
ACGTTTA	185	1.8189894E-12	17.0	26
GTATTGG	1550	0.0	16.351614	1
ACCGTTA	710	0.0	15.633802	8
TTATGCG	420	0.0	15.416668	4
TAGTACT	670	0.0	15.186567	4
GGACCGT	895	0.0	14.882682	6
TGTTACG	425	0.0	14.799999	16
TCTACAC	470	0.0	14.563828	3
GCTTATG	610	0.0	14.557376	2
ATTGGAC	1915	0.0	14.297651	3
CCGTTTA	740	0.0	14.25	27
TTGGACC	2385	0.0	14.1174	4
GTATTAG	745	0.0	13.90604	1
TAGGTCG	190	7.1413524E-9	13.631579	21
GTATCAA	17215	0.0	13.508278	2
CGTTCGC	630	0.0	13.5079365	23
CGTTTAC	790	0.0	13.348101	28
>>END_MODULE