FastQCFastQC Report
Wed 25 May 2016
SRR2088622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353005
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT234790.9978304338494818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT208980.8881409091778385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165520.7034409191650677No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89800.3816396480245473No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46830.19902210152549613No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46180.19625967645627612No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG46170.19621717760905738No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG43040.1829150384295826No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA40150.1706328715833583No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG35490.15082840877941187No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25160.10692709960242329No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.10688460075520452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75800.021.160291
GTACTAA801.6178437E-518.51
TCACGTT1601.8189894E-1218.524
CGAATTA852.7250782E-517.41176415
ACGTTTA1901.8189894E-1216.55263126
TAATACT6800.016.0514724
ATAACGC1101.45307495E-515.1363643
TTCGTTA3200.015.0312530
ATCATAC1651.6474587E-814.5757573
ATACTGG6150.014.4390246
ACTGACG908.2785566E-414.3888888
GTATCAA111950.014.3108542
GTATTGG11650.014.1330481
CACGTTT2101.364242E-1014.09523825
CGTTTAT2101.364242E-1014.09523827
TATACGG2500.014.0599992
CTAGACA1203.3032476E-513.8749994
CTGTGCG1753.5706762E-813.7428579
TCTATAC2304.0017767E-1113.6739133
TCGCATA950.001245751713.63157918
Produced by FastQC (version 0.11.5)