FastQCFastQC Report
Wed 25 May 2016
SRR2088621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477923
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT373671.0744056150754344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323980.9315329867855039No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263770.7584124202864756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140880.40506934742373535No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG74130.21314445431943146No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA70880.2037997966027425No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70880.2037997966027425No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG63930.18381660548551534No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA58680.1687213891739409No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG53440.15365492565534083No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42260.12150930310993084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.11434985765929838No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37280.10719041220866592No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC34820.10011722513695673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117550.019.987241
GTATTGG18200.016.3653851
TTGGACC22750.015.2879114
ATTGGAC20000.015.26253
ACCGTTA4900.015.102048
TATACTG5600.014.8660715
TCGGGTA3800.014.60526425
AAGACCG3850.014.4155845
GTCTATA2700.014.3888891
CGGGTAA4050.014.16049426
ATTAGAC2800.013.8753
TTCGCTA4300.013.76744226
TATTGGA23100.013.6948052
CTGTGCG3700.013.59
TGGACCC25500.013.4941185
GACCGTT5800.013.3965517
CGCTACC5000.013.3228
GTATCAA176650.013.3107852
CGTTCGC5200.013.16346223
TGCGACG2254.0745363E-1013.15555622
Produced by FastQC (version 0.11.5)