##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3477923
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.691029099839184	31.0	31.0	34.0	30.0	34.0
2	31.863313535118518	31.0	31.0	34.0	30.0	34.0
3	31.95048107735565	31.0	31.0	34.0	30.0	34.0
4	35.63648936448564	37.0	35.0	37.0	33.0	37.0
5	35.53847080570789	37.0	35.0	37.0	33.0	37.0
6	35.56930558842159	37.0	35.0	37.0	33.0	37.0
7	35.44470909793	37.0	35.0	37.0	33.0	37.0
8	35.46708394636684	37.0	35.0	37.0	33.0	37.0
9	37.07833410917953	39.0	37.0	39.0	33.0	39.0
10	36.94706524555029	39.0	37.0	39.0	33.0	39.0
11	36.9827652883632	39.0	37.0	39.0	33.0	39.0
12	36.8921045693076	39.0	37.0	39.0	33.0	39.0
13	36.94073819345627	39.0	37.0	39.0	33.0	39.0
14	37.97919361641991	40.0	37.0	41.0	33.0	41.0
15	37.94078189770159	40.0	37.0	41.0	33.0	41.0
16	37.84418861487158	40.0	37.0	41.0	32.0	41.0
17	37.86660400474651	40.0	37.0	41.0	33.0	41.0
18	37.83892081567073	40.0	37.0	41.0	32.0	41.0
19	37.85772111688499	40.0	37.0	41.0	32.0	41.0
20	37.7426791794988	40.0	37.0	41.0	32.0	41.0
21	37.65117916641628	40.0	37.0	41.0	32.0	41.0
22	37.50115744368119	39.0	36.0	41.0	32.0	41.0
23	37.37955325635444	39.0	36.0	41.0	31.0	41.0
24	37.23787616919638	39.0	36.0	41.0	31.0	41.0
25	37.05811169482475	39.0	36.0	41.0	31.0	41.0
26	37.20996410788853	39.0	36.0	41.0	31.0	41.0
27	37.131265125766156	39.0	36.0	41.0	31.0	41.0
28	37.017004114237146	39.0	36.0	41.0	31.0	41.0
29	36.869037928671794	39.0	35.0	41.0	30.0	41.0
30	36.76050188575193	39.0	35.0	41.0	30.0	41.0
31	36.58369607377737	39.0	35.0	40.0	30.0	41.0
32	36.46350824903254	39.0	35.0	40.0	30.0	41.0
33	36.257630775609464	38.0	35.0	40.0	30.0	41.0
34	36.12017373587627	38.0	35.0	40.0	30.0	41.0
35	35.927845728614464	38.0	35.0	40.0	29.0	41.0
36	35.78315333605718	38.0	35.0	40.0	27.0	41.0
37	35.61813156875526	38.0	35.0	40.0	27.0	41.0
38	35.53638766585689	38.0	35.0	40.0	26.0	41.0
39	35.404043447770405	38.0	34.0	40.0	26.0	41.0
40	35.23376883271999	38.0	34.0	40.0	25.0	41.0
41	35.11341826716693	38.0	34.0	40.0	24.0	41.0
42	34.90638521899421	38.0	34.0	40.0	23.0	41.0
43	34.55603444929632	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	5.0
14	10.0
15	32.0
16	46.0
17	98.0
18	266.0
19	524.0
20	1198.0
21	2413.0
22	4352.0
23	7629.0
24	12418.0
25	19112.0
26	27864.0
27	39989.0
28	54041.0
29	71485.0
30	90406.0
31	111473.0
32	136082.0
33	168969.0
34	208819.0
35	259153.0
36	337004.0
37	477598.0
38	661512.0
39	785418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02887758009594	20.987324906273084	13.586471005827327	23.39732650780365
2	18.735176138172122	23.136222394802875	35.11920764203233	23.009393824992674
3	19.995612323792102	24.80937617077779	31.922443366342495	23.27256813908761
4	14.05404892517747	16.093110744544948	35.968823921633685	33.884016408643895
5	13.41165402454281	37.96064490214418	34.58912115075578	14.038579922557226
6	32.83091661316251	36.61719940320703	14.049534736680483	16.502349246949976
7	26.301991159666272	32.47616465344402	21.888408685298668	19.333435501591037
8	26.80861537187569	34.534375832932476	19.34436156292132	19.312647232270525
9	24.718229817048854	14.4770025098313	21.90373392395404	38.90103374916581
10	16.52621406511875	27.194535359178452	33.60016883640034	22.67908173930245
11	33.23431829859373	22.39365276344531	23.463256662094015	20.908772275866948
12	20.787521747893788	26.640756566490975	31.382782195005465	21.18893949060977
13	31.297386399871417	20.74220734616609	26.267976605577527	21.69242964838497
14	20.67713977566496	22.575082887113947	27.73086120653045	29.016916130690646
15	23.208679433098432	29.11059273020133	25.159700200378214	22.52102763632202
16	22.224270060032953	27.922613582876902	26.49621627620853	23.35690008088161
17	21.78093074516026	26.55616584956021	27.33516526961638	24.32773813566315
18	23.06546177129281	25.378566460499556	29.2642189030637	22.291752865143938
19	22.904446130636014	24.97392840497044	30.142616728432458	21.979008735961088
20	23.773815579010808	24.647641710296632	29.858682897809985	21.719859812882575
21	22.8461067136909	25.30366543480117	30.184682064554046	21.66554578695388
22	21.817648061788603	25.6363352495153	29.97625306828242	22.569763620413678
23	22.388448507916937	25.789731400033872	30.641535192124724	21.180284899924466
24	22.505242352979064	25.821071944375994	28.908086809282434	22.765598893362505
25	22.51450075231683	25.958596553172686	29.442888758606788	22.084013935903698
26	22.321281983528678	26.38137762106867	29.54867028395971	21.74867011144295
27	21.49130386152885	26.57479765940764	29.416953739343853	22.516944739719655
28	21.813248884463516	26.299949711365088	29.427160980849777	22.45964042332162
29	21.862272396484915	26.281087879173864	29.130374651767738	22.726265072573486
30	21.867332888048413	26.50323195769429	30.264327301093207	21.365107853164087
31	22.506478723076963	25.97771716050068	30.147217175308366	21.368586941113993
32	22.069637539416483	26.008137615467625	29.275116211601006	22.647108633514886
33	21.86776417994303	25.94663539129532	29.867768780389902	22.317831648371744
34	21.279280766135418	26.346097944089042	30.146814636206727	22.22780665356881
35	20.996497047231927	27.86286527907605	29.460571726286062	21.680065947405964
36	22.470278956722158	26.042468450279088	29.515259538523424	21.971993054475327
37	21.834698468022438	26.357771578036658	29.43664940253134	22.370880551409563
38	21.55300735525197	25.329801723614924	30.582735730492022	22.534455190641083
39	21.143826358432893	25.123327917265563	31.19640659094523	22.536439133356318
40	20.380382199375894	25.732743364358555	30.962416361719335	22.924458074546216
41	21.337303902357814	24.873322382352917	30.94573974179417	22.843633973495102
42	20.11197487695961	26.44348365389343	30.562234989101256	22.882306480045706
43	20.295388943343482	26.41677230922019	29.938213123177253	23.349625624259076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	461.5
2	687.0
3	2007.5
4	3328.0
5	3328.0
6	5155.0
7	6982.0
8	7451.0
9	7920.0
10	11447.5
11	14975.0
12	14975.0
13	26428.0
14	37881.0
15	57987.0
16	78093.0
17	72511.5
18	66930.0
19	66930.0
20	71953.0
21	76976.0
22	62946.0
23	48916.0
24	54902.5
25	60889.0
26	60889.0
27	67246.0
28	73603.0
29	80856.5
30	88110.0
31	95004.5
32	101899.0
33	101899.0
34	111631.0
35	121363.0
36	132901.0
37	144439.0
38	154098.5
39	163758.0
40	163758.0
41	171056.5
42	178355.0
43	189095.0
44	199835.0
45	215468.0
46	231101.0
47	231101.0
48	285823.5
49	340546.0
50	325656.5
51	310767.0
52	292153.0
53	273539.0
54	273539.0
55	246894.0
56	220249.0
57	193012.0
58	165775.0
59	148383.0
60	130991.0
61	130991.0
62	116144.0
63	101297.0
64	87838.0
65	74379.0
66	63511.5
67	52644.0
68	52644.0
69	45267.0
70	37890.0
71	31772.0
72	25654.0
73	20876.5
74	16099.0
75	16099.0
76	12993.0
77	9887.0
78	7675.5
79	5464.0
80	4246.0
81	3028.0
82	3028.0
83	2341.5
84	1655.0
85	1228.5
86	802.0
87	635.5
88	469.0
89	469.0
90	389.0
91	309.0
92	212.5
93	116.0
94	85.5
95	55.0
96	55.0
97	39.5
98	24.0
99	16.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3477923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.66061933832236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2573061942278	38.863062933540824
2	12.172482221106137	12.089860119696972
3	4.021879517045452	5.9918708316177
4	1.7642296186840407	3.5045094207544696
5	0.9416236637696499	2.338080716321052
6	0.5776378802581629	1.7211512932137636
7	0.3738703028091985	1.2996641552797643
8	0.2630293392116707	1.0449759911520995
9	0.2025461077722244	0.9052708640881726
>10	1.2114189486053146	11.420836729422168
>50	0.12207033187077773	4.206716517897573
>100	0.08319773684189195	7.586748006152234
>500	0.004992665671507122	1.732314284176963
>1k	0.0031349296064130745	2.9870291127832953
>5k	3.4832551182367494E-4	1.1299669817623008
>10k+	2.3221700788244993E-4	3.1779420421405935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37367	1.0744056150754344	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32398	0.9315329867855039	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26377	0.7584124202864756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14088	0.40506934742373535	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7413	0.21314445431943146	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7088	0.2037997966027425	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7088	0.2037997966027425	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6393	0.18381660548551534	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5868	0.1687213891739409	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5344	0.15365492565534083	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4226	0.12150930310993084	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3977	0.11434985765929838	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3728	0.10719041220866592	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3482	0.10011722513695673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.8752792974427555E-5	0.0
5	0.0	0.0	0.0	2.8752792974427555E-5	0.0
6	0.0	0.0	5.750558594885511E-5	2.8752792974427555E-5	0.0
7	2.8752792974427555E-5	0.0	5.750558594885511E-5	2.8752792974427555E-5	0.0
8	2.8752792974427555E-5	0.0	5.750558594885511E-5	2.8752792974427555E-5	0.0
9	2.8752792974427555E-5	0.0	5.750558594885511E-5	1.1501117189771022E-4	0.0
10	2.8752792974427555E-5	0.0	5.750558594885511E-5	1.7251675784656531E-4	0.0
11	2.8752792974427555E-5	0.0	5.750558594885511E-5	3.737863086675582E-4	0.0
12	2.8752792974427555E-5	0.0	5.750558594885511E-5	9.200893751816817E-4	0.0
13	5.750558594885511E-5	0.0	5.750558594885511E-5	0.001035100547079392	0.0
14	5.750558594885511E-5	0.0	8.625837892328266E-5	0.0012076173049259573	0.0
15	1.1501117189771022E-4	0.0	8.625837892328266E-5	0.0014376396487213777	0.0
16	1.1501117189771022E-4	0.0	1.1501117189771022E-4	0.0020414483011843565	0.0
17	1.1501117189771022E-4	0.0	1.1501117189771022E-4	0.0028752792974427554	0.0
18	1.1501117189771022E-4	0.0	1.1501117189771022E-4	0.003622851914777872	0.0
19	1.1501117189771022E-4	0.0	1.1501117189771022E-4	0.004485435704010698	0.0
20	1.1501117189771022E-4	0.0	1.4376396487213777E-4	0.007907018067967578	0.0
21	1.1501117189771022E-4	0.0	1.4376396487213777E-4	0.013197531975262248	0.0
22	1.1501117189771022E-4	0.0	1.4376396487213777E-4	0.022254661762206928	0.0
23	1.1501117189771022E-4	0.0	1.4376396487213777E-4	0.029184084869043968	0.0
24	1.1501117189771022E-4	0.0	1.4376396487213777E-4	0.04103023557450812	0.0
25	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.04896600643545013	0.0
26	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.06170349372312153	0.0
27	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.11403357693657967	0.0
28	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.23424900436266127	0.0
29	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.38804769398287425	0.0
30	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.5975405435945534	0.0
31	2.3002234379542044E-4	0.0	1.4376396487213777E-4	0.9154314227198245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11755	0.0	19.98724	1
GTATTGG	1820	0.0	16.365385	1
TTGGACC	2275	0.0	15.287911	4
ATTGGAC	2000	0.0	15.2625	3
ACCGTTA	490	0.0	15.10204	8
TATACTG	560	0.0	14.866071	5
TCGGGTA	380	0.0	14.605264	25
AAGACCG	385	0.0	14.415584	5
GTCTATA	270	0.0	14.388889	1
CGGGTAA	405	0.0	14.160494	26
ATTAGAC	280	0.0	13.875	3
TTCGCTA	430	0.0	13.767442	26
TATTGGA	2310	0.0	13.694805	2
CTGTGCG	370	0.0	13.5	9
TGGACCC	2550	0.0	13.494118	5
GACCGTT	580	0.0	13.396551	7
CGCTACC	500	0.0	13.32	28
GTATCAA	17665	0.0	13.310785	2
CGTTCGC	520	0.0	13.163462	23
TGCGACG	225	4.0745363E-10	13.155556	22
>>END_MODULE