FastQCFastQC Report
Wed 25 May 2016
SRR2088620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156266
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT452131.0878273912208698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT393100.9458008703004092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT322470.7758646823855836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170400.4099833841241153No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG97200.23386376136657278No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA93320.22452845895811288No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87150.20968340332404137No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78810.1896173151574033No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA74830.1800414121714058No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG65630.15790615903794417No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54690.13158445585532783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47370.11397249357957359No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA44980.10822213977642432No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC43920.10567177365452549No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.10461313111335992No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG43220.1039875696117621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142450.020.8051951
TAATACT10700.015.9065424
GTATTGG21750.015.6505751
AATACTG11250.015.2933345
CTAATAC10200.014.5098053
ATTGGAC24650.014.3346863
TTATGCG3750.013.8133334
CCGTTTA6700.013.8059727
GTATCAA214300.013.7951472
ACCGTTA6450.013.7674428
ATACTGG9950.013.7587946
TAGAACA4250.013.4941184
TCTAATA9950.013.3869342
TTGGACC29850.013.2010054
GTATTAG8150.013.1656441
TTCGTTA6650.013.07518930
TCGCTAA850.00941014213.05882414
GACCGTT7200.012.8472227
CGTTCGC6950.012.77697923
CTCTAAT10000.012.7650011
Produced by FastQC (version 0.11.5)