FastQCFastQC Report
Wed 25 May 2016
SRR2088619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3941254
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT507041.2864940955340611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT425331.0791742932579327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT349250.8861392846033267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182330.46261925772863155No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG100670.255426318628538No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94870.2407101902084971No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA94610.24005050169311595No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG94090.23873112466235366No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA84200.21363758844266315No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG73120.1855247086333436No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54510.13830623451317778No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50300.1276243550910446No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47240.11986032871771268No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA47170.1196827202712639No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.11486191958194017No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC45140.1145320753242496No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44760.11356791518638484No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41390.10501733712163693No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40860.10367258745566767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144500.021.2013851
TAATACT9250.017.84
TCTAATA9400.016.9255312
CTAATAC9550.016.8534053
ACCGTTA7400.016.58
GTATTGG20550.016.2944051
TGTTACG4250.015.670588516
ATAACGC1902.7284841E-1115.5789483
CCGTTAT8050.015.3975169
CTCTAAT9200.015.2826071
AATACTG11150.015.2645745
ATTGGAC22750.015.0439563
TATACCG2109.094947E-1214.976195
CCGTTTA8050.014.93788827
GACCGTT8300.014.9337357
TTGGACC28150.014.5896984
TACTGGT10850.014.4930887
TTCGCTA5700.014.28070326
GTATTAG7400.014.251
ATACTGG10050.014.174136
Produced by FastQC (version 0.11.5)