##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088619_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3941254
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71410063903519	31.0	31.0	34.0	30.0	34.0
2	31.884892219582905	31.0	31.0	34.0	30.0	34.0
3	31.958979045755488	31.0	31.0	34.0	30.0	34.0
4	35.6396215519223	37.0	35.0	37.0	33.0	37.0
5	35.555001783696255	37.0	35.0	37.0	33.0	37.0
6	35.59379527429595	37.0	35.0	37.0	33.0	37.0
7	35.45674574640457	37.0	35.0	37.0	33.0	37.0
8	35.48968399397755	37.0	35.0	37.0	33.0	37.0
9	37.095523911932595	39.0	37.0	39.0	33.0	39.0
10	36.96091193310556	39.0	37.0	39.0	33.0	39.0
11	37.00622263878451	39.0	37.0	39.0	33.0	39.0
12	36.90945013947338	39.0	37.0	39.0	33.0	39.0
13	36.955053137909914	39.0	37.0	39.0	33.0	39.0
14	37.973969198635764	40.0	37.0	41.0	33.0	41.0
15	37.94248683287096	40.0	37.0	41.0	33.0	41.0
16	37.844389374549316	40.0	37.0	41.0	32.0	41.0
17	37.8860319583564	40.0	37.0	41.0	33.0	41.0
18	37.86913860410925	40.0	37.0	41.0	33.0	41.0
19	37.89838056618528	40.0	37.0	41.0	32.0	41.0
20	37.78344658831935	40.0	37.0	41.0	32.0	41.0
21	37.68924915775538	40.0	37.0	41.0	32.0	41.0
22	37.534152581893984	39.0	36.0	41.0	32.0	41.0
23	37.40581627065903	39.0	36.0	41.0	32.0	41.0
24	37.26223709509714	39.0	36.0	41.0	31.0	41.0
25	37.06396771179934	39.0	36.0	41.0	31.0	41.0
26	37.20518266521265	39.0	36.0	41.0	31.0	41.0
27	37.131459175176225	39.0	36.0	41.0	31.0	41.0
28	37.00986767155834	39.0	36.0	41.0	31.0	41.0
29	36.86032135964848	39.0	35.0	41.0	30.0	41.0
30	36.72677782249	39.0	35.0	41.0	30.0	41.0
31	36.528059851001736	39.0	35.0	40.0	30.0	41.0
32	36.401117258618704	38.0	35.0	40.0	30.0	41.0
33	36.19061217571869	38.0	35.0	40.0	30.0	41.0
34	36.04416893709464	38.0	35.0	40.0	29.0	41.0
35	35.849638719047284	38.0	35.0	40.0	28.0	41.0
36	35.70749284364824	38.0	35.0	40.0	27.0	41.0
37	35.52706016917458	38.0	35.0	40.0	27.0	41.0
38	35.42479703160466	38.0	34.0	40.0	26.0	41.0
39	35.28052467564892	38.0	34.0	40.0	25.0	41.0
40	35.09044431036416	38.0	34.0	40.0	24.0	41.0
41	34.946600498217066	38.0	34.0	40.0	24.0	41.0
42	34.712211900070386	38.0	33.0	40.0	23.0	41.0
43	34.34434598734312	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	6.0
14	12.0
15	15.0
16	46.0
17	84.0
18	238.0
19	596.0
20	1263.0
21	2748.0
22	5111.0
23	8864.0
24	14100.0
25	22273.0
26	32224.0
27	45470.0
28	61702.0
29	81371.0
30	102901.0
31	126578.0
32	155414.0
33	192224.0
34	238982.0
35	295938.0
36	386261.0
37	552974.0
38	739273.0
39	874581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20253249346528	20.791225330821103	13.853382705098428	23.15285947061519
2	19.045156693783248	23.12685251952805	34.825920887108516	23.002069899580185
3	20.04159082363126	24.421110641435444	32.33980859898905	23.197489935944247
4	13.780131907255914	15.95738310700097	35.935745323696466	34.32673966204665
5	13.093776752272246	38.25559073330468	34.60449389965732	14.046138614765757
6	32.77398513265067	36.64678805273652	13.58022091446022	16.999005900152593
7	26.312513732938807	32.601704939595365	21.45299440228922	19.632786925176607
8	27.2608159737992	34.24476067769294	18.832001185409517	19.662422163098345
9	24.596740022338068	14.353528090298164	21.68330181206286	39.366430075300904
10	16.843953726402816	27.078716570918797	33.14698824282829	22.930341459850087
11	33.41601429392777	22.216685349383724	23.332066393082	21.035233963606505
12	21.11117426078096	26.900346945413823	30.815877383188194	21.172601410617027
13	31.645461063915192	20.56946849911221	26.21592011070588	21.569150326266715
14	20.62528829656754	22.655606565829046	27.671827291516866	29.04727784608655
15	23.15811160610303	29.062805898833215	25.441598029459662	22.337484465604092
16	22.028420396148025	27.83814491529853	26.623049415236878	23.510385273316565
17	21.99492851767483	26.37178420878228	27.039033769455102	24.594253504087785
18	23.096659083631756	25.14879781917126	29.452656438788267	22.30188665840872
19	23.03071560472885	24.558934795879686	30.462766419012834	21.94758318037863
20	23.952655677609208	24.12678807303462	30.262144992431345	21.658411256924825
21	22.761486572547724	25.023406255978426	30.725322448134527	21.489784723339323
22	21.716235492561506	25.407065872942976	30.2918563482587	22.584842286236817
23	22.3877476559491	25.676802357828244	31.19798926940512	20.73746071681754
24	22.48210848628381	25.703392879525143	28.882507953052507	22.931990681138544
25	22.405229401606697	25.781845067585092	29.843572629422006	21.96935290138621
26	22.164823683020686	26.485276006063046	29.892364206925002	21.457536103991266
27	21.29154832446729	26.52891693861903	29.573100338115736	22.606434398797944
28	21.582014252316647	26.155051158844365	29.804676379649724	22.458258209189257
29	21.980288507160413	26.070433420429133	29.23584727094473	22.713430801465726
30	21.80009205192053	26.233858563797206	30.762467986077528	21.203581398204737
31	22.307113421261356	25.8081057450243	30.69449469635806	21.19028613735628
32	21.934059565813318	25.76837219829019	29.640033349791718	22.657534886104777
33	21.687259943155148	25.82853071636591	30.19186279290804	22.292346547570897
34	21.077580891766935	26.30769293224948	30.644180760742646	21.970545415240935
35	20.768719803392525	28.10580084409683	29.6319648517959	21.493514500714745
36	22.6222922958023	25.81876225181123	29.6836742823477	21.875271170038772
37	21.66828121202034	26.468301713109582	29.579215143200617	22.284201931669465
38	21.484811686838757	25.113428365692748	30.939974941985472	22.461785005483026
39	20.982661863457672	25.016149682309237	31.657690674084947	22.343497780148144
40	20.097664347438656	25.729171476895424	31.1778941423212	22.995270033344717
41	21.247983509816926	24.645633090381892	31.34695201070522	22.75943138909596
42	20.006754195492093	26.516230621015545	30.71639128054168	22.760623902950687
43	20.265275975615882	26.28414712677742	30.02894510224411	23.421631795362593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	490.0
2	768.0
3	2301.5
4	3835.0
5	3835.0
6	5879.5
7	7924.0
8	8615.0
9	9306.0
10	13591.5
11	17877.0
12	17877.0
13	32669.5
14	47462.0
15	74172.5
16	100883.0
17	91562.0
18	82241.0
19	82241.0
20	87202.5
21	92164.0
22	71723.0
23	51282.0
24	57198.0
25	63114.0
26	63114.0
27	69567.5
28	76021.0
29	82567.5
30	89114.0
31	98204.5
32	107295.0
33	107295.0
34	118832.0
35	130369.0
36	143812.5
37	157256.0
38	169565.0
39	181874.0
40	181874.0
41	189783.0
42	197692.0
43	214563.0
44	231434.0
45	247409.0
46	263384.0
47	263384.0
48	336560.0
49	409736.0
50	388222.5
51	366709.0
52	339806.0
53	312903.0
54	312903.0
55	280423.0
56	247943.0
57	213545.5
58	179148.0
59	160246.0
60	141344.0
61	141344.0
62	125399.5
63	109455.0
64	95512.5
65	81570.0
66	70468.5
67	59367.0
68	59367.0
69	51521.0
70	43675.0
71	37335.0
72	30995.0
73	25486.5
74	19978.0
75	19978.0
76	15992.0
77	12006.0
78	9463.0
79	6920.0
80	5415.5
81	3911.0
82	3911.0
83	2926.0
84	1941.0
85	1476.0
86	1011.0
87	787.0
88	563.0
89	563.0
90	468.5
91	374.0
92	245.5
93	117.0
94	82.0
95	47.0
96	47.0
97	36.0
98	25.0
99	17.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3941254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.302630980429775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35891147319786	32.30182854117995
2	12.322855535920477	10.425784207223803
3	4.431599713249284	5.624049819676886
4	2.0642976639807418	3.4930088925256224
5	1.104097061635486	2.335310528247139
6	0.7227459090570089	1.8344432090072342
7	0.46927962656940736	1.3896234008579669
8	0.34561344372647934	1.1696286377469431
9	0.261818168962276	0.9968037647024676
>10	1.6141077852512553	13.177987607906037
>50	0.16888879860690045	5.012378347320974
>100	0.12055475724169014	9.877666936401233
>500	0.009330670768940447	2.6991419851329987
>1k	0.005177017322100048	4.288354040135551
>5k	4.213851308686085E-4	1.3896406174002198
>10k+	3.009893791918632E-4	3.9843494645349677
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	50704	1.2864940955340611	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42533	1.0791742932579327	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34925	0.8861392846033267	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18233	0.46261925772863155	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10067	0.255426318628538	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9487	0.2407101902084971	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9461	0.24005050169311595	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9409	0.23873112466235366	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8420	0.21363758844266315	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7312	0.1855247086333436	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5451	0.13830623451317778	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5030	0.1276243550910446	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4724	0.11986032871771268	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4717	0.1196827202712639	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4527	0.11486191958194017	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4514	0.1145320753242496	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4476	0.11356791518638484	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4139	0.10501733712163693	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4086	0.10367258745566767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.537263520696712E-5	0.0	0.0	0.0	0.0
6	5.074527041393424E-5	0.0	0.0	0.0	0.0
7	5.074527041393424E-5	0.0	0.0	2.537263520696712E-5	0.0
8	5.074527041393424E-5	0.0	0.0	2.537263520696712E-5	0.0
9	7.611790562090137E-5	0.0	0.0	1.2686317603483562E-4	0.0
10	1.0149054082786848E-4	0.0	0.0	2.0298108165573697E-4	0.0
11	1.2686317603483562E-4	0.0	0.0	4.313347985184411E-4	0.0
12	1.2686317603483562E-4	0.0	0.0	8.37296961829915E-4	0.0
13	1.2686317603483562E-4	0.0	0.0	9.387875026577835E-4	0.0
14	1.5223581124180273E-4	0.0	0.0	0.0011163959491065534	0.0
15	1.5223581124180273E-4	0.0	0.0	0.001446240206797126	0.0
16	1.5223581124180273E-4	0.0	0.0	0.0021820466277991724	0.0
17	1.5223581124180273E-4	0.0	0.0	0.00317157940087089	0.0
18	1.5223581124180273E-4	0.0	0.0	0.00405962163311474	0.0
19	1.5223581124180273E-4	0.0	0.0	0.005099899676600392	0.0
20	1.7760844644876985E-4	0.0	0.0	0.009032658133680295	0.0
21	1.7760844644876985E-4	0.0	0.0	0.015578798017077813	0.0
22	1.7760844644876985E-4	0.0	0.0	0.024002512905790897	0.0
23	1.7760844644876985E-4	0.0	0.0	0.030624770694809317	0.0
24	1.7760844644876985E-4	0.0	0.0	0.04112904167049371	0.0
25	1.7760844644876985E-4	0.0	0.0	0.0482333795284445	0.0
26	1.7760844644876985E-4	0.0	0.0	0.0593465937490961	0.0
27	1.7760844644876985E-4	0.0	0.0	0.1183125979700877	0.0
28	1.7760844644876985E-4	0.0	0.0	0.25184877706435566	0.0
29	1.7760844644876985E-4	0.0	0.0	0.41829326402205996	0.0
30	1.7760844644876985E-4	0.0	0.0	0.6440082268232395	0.0
31	2.0298108165573697E-4	0.0	0.0	0.9653780243546851	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14450	0.0	21.201385	1
TAATACT	925	0.0	17.8	4
TCTAATA	940	0.0	16.925531	2
CTAATAC	955	0.0	16.853405	3
ACCGTTA	740	0.0	16.5	8
GTATTGG	2055	0.0	16.294405	1
TGTTACG	425	0.0	15.6705885	16
ATAACGC	190	2.7284841E-11	15.578948	3
CCGTTAT	805	0.0	15.397516	9
CTCTAAT	920	0.0	15.282607	1
AATACTG	1115	0.0	15.264574	5
ATTGGAC	2275	0.0	15.043956	3
TATACCG	210	9.094947E-12	14.97619	5
CCGTTTA	805	0.0	14.937888	27
GACCGTT	830	0.0	14.933735	7
TTGGACC	2815	0.0	14.589698	4
TACTGGT	1085	0.0	14.493088	7
TTCGCTA	570	0.0	14.280703	26
GTATTAG	740	0.0	14.25	1
ATACTGG	1005	0.0	14.17413	6
>>END_MODULE