FastQCFastQC Report
Wed 25 May 2016
SRR2088618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078720
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT422931.3737202473755328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT378121.2281727471156845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT298640.9700135121089283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157000.5099521879222534No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG85510.27774529674669995No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA84100.27316547136472297No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA84100.27316547136472297No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG81500.2647204032844819No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75880.24646606381872987No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG64780.21041211932231577No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46580.1512966427606278No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.14353367633302153No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43590.1415848144683505No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA41750.135608304750026No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40540.13167809998960608No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC40080.13018397256002495No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39590.12859240203721028No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG38250.1242399438727783No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC37790.12274581644319718No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC34600.11238436752936286No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34020.11050046772684752No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC31510.10234772892630704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131800.022.6968881
TGTTACG3700.018.016
GTATTGG17450.017.5988521
TAGCGAA5050.016.85148610
AGTTCCG4200.016.73809432
CGGGTAA3850.016.33766226
GTCGGGT3900.016.12820424
ATTGGAC20450.016.102693
CGGTATA1504.6802597E-916.03333326
CGTTATT4050.015.98765510
ACCGTTA4950.015.696978
TCGGGTA4100.015.34146325
GTATTAG6300.015.2698411
TACACAG4000.015.2625015
TAATACT6700.015.1865674
GTATCAA198100.015.11004452
GTAGTGT1353.978148E-715.0740736
TGTCGGG5600.014.86607223
TTGGACC24850.014.8148894
TTAACGG3400.014.69117635
Produced by FastQC (version 0.11.5)