FastQCFastQC Report
Wed 25 May 2016
SRR2088617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594611
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66041.110642083647965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63681.070952269635106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48530.8161638449339148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25400.42717003217229416No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14910.2507521724286971No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG14200.23881159278923533No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG14050.2362889351189265No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13820.23242086002445297No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA13610.22888913928602062No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG11560.19441281779180003No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8420.14160518389333532No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8170.1374007544428206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.13134637603407942No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC7180.12075121381878236No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.11822855614847354No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG6800.114360481054No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6750.11351959516389706No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA6690.11251053209577354No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA6380.10729703957713531No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.10393349601672355No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC6090.10241990141453824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA358.8643417E-426.428571
GGTATCA20650.021.7699761
GACCGTC603.7223934E-521.5833347
CGACCGC450.003823668720.55555510
CCGACCG450.003823668720.5555559
ATTAGAG1001.2865712E-820.3499983
TTAGACC555.139081E-420.1818184
TGTTACG555.139081E-420.18181816
GTATTAG1103.8422513E-818.51
TGCACCG609.229669E-418.55
CTATCCT500.00703098718.4999984
TAGGTCG500.00703098718.49999821
CATTGCG500.00703098718.49999829
AGTTCCG500.00703098718.4999985
ACCGTCG500.00703098718.4999988
TAACGGC752.0651534E-417.26666636
TTAACGG752.0651534E-417.26666635
GCCTAGT752.0651534E-417.2666661
GGGTCGA650.001578721817.07692310
TGTGCGC650.001578721817.07692310
Produced by FastQC (version 0.11.5)