##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594611
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.725097584807546	31.0	31.0	34.0	30.0	34.0
2	31.902833953626825	31.0	31.0	34.0	30.0	34.0
3	31.979532837434895	31.0	31.0	34.0	30.0	34.0
4	35.668541281611006	37.0	35.0	37.0	33.0	37.0
5	35.57841849545333	37.0	35.0	37.0	33.0	37.0
6	35.611359359312225	37.0	35.0	37.0	33.0	37.0
7	35.473142945556	37.0	35.0	37.0	33.0	37.0
8	35.503900869644184	37.0	35.0	37.0	33.0	37.0
9	37.11291752086658	39.0	37.0	39.0	33.0	39.0
10	36.98786769837759	39.0	37.0	39.0	33.0	39.0
11	37.027554148846896	39.0	37.0	39.0	33.0	39.0
12	36.930998585629936	39.0	37.0	39.0	33.0	39.0
13	36.9669582298343	39.0	37.0	39.0	33.0	39.0
14	38.000110996937494	40.0	37.0	41.0	33.0	41.0
15	37.95850228132342	40.0	37.0	41.0	33.0	41.0
16	37.84500286742089	40.0	37.0	41.0	32.0	41.0
17	37.88802427133033	40.0	37.0	41.0	33.0	41.0
18	37.869846000158084	40.0	37.0	41.0	33.0	41.0
19	37.88282927830127	40.0	37.0	41.0	32.0	41.0
20	37.783973051288996	40.0	37.0	41.0	32.0	41.0
21	37.671077393455555	40.0	37.0	41.0	32.0	41.0
22	37.52502224143179	39.0	36.0	41.0	32.0	41.0
23	37.3836087795214	39.0	36.0	41.0	31.0	41.0
24	37.246544379434624	39.0	36.0	41.0	31.0	41.0
25	37.05205251836915	39.0	36.0	41.0	31.0	41.0
26	37.206106176979574	39.0	36.0	41.0	31.0	41.0
27	37.12472019522007	39.0	36.0	41.0	31.0	41.0
28	36.99271288287637	39.0	36.0	41.0	31.0	41.0
29	36.853401635691235	39.0	35.0	41.0	30.0	41.0
30	36.72287091897056	39.0	35.0	41.0	30.0	41.0
31	36.53537186496718	39.0	35.0	40.0	30.0	41.0
32	36.41869390240006	38.0	35.0	40.0	30.0	41.0
33	36.19891660261919	38.0	35.0	40.0	30.0	41.0
34	36.05431954672887	38.0	35.0	40.0	29.0	41.0
35	35.87136464007561	38.0	35.0	40.0	29.0	41.0
36	35.70474814626706	38.0	35.0	40.0	27.0	41.0
37	35.53346978108377	38.0	35.0	40.0	27.0	41.0
38	35.44584610779148	38.0	34.0	40.0	26.0	41.0
39	35.2968613093266	38.0	34.0	40.0	25.0	41.0
40	35.10816987913106	38.0	34.0	40.0	24.0	41.0
41	34.96763934740528	38.0	34.0	40.0	24.0	41.0
42	34.72818699956778	38.0	33.0	40.0	23.0	41.0
43	34.36237304725274	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	2.0
16	11.0
17	14.0
18	46.0
19	103.0
20	198.0
21	391.0
22	703.0
23	1241.0
24	2012.0
25	3277.0
26	4808.0
27	6679.0
28	9312.0
29	12312.0
30	15174.0
31	19056.0
32	23515.0
33	28975.0
34	36332.0
35	45408.0
36	58685.0
37	83109.0
38	112545.0
39	130700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.060944045771095	20.837656888284947	13.770851867859829	23.330547198084126
2	19.242664531937688	22.706946221983785	35.12077644039549	22.929612805683046
3	20.031751851210284	24.339778443385676	32.33475330930642	23.293716396097615
4	14.041448947294954	16.111037300016314	35.647002830421904	34.20051092226682
5	13.25370704544652	37.73744515321782	34.72186017413065	14.286987627205013
6	32.74762828134696	36.33198847649976	13.618819698929215	17.30156354322406
7	26.007759694993872	32.73686494195365	21.513056435215628	19.742318927836855
8	26.699976959726612	34.50928422111262	19.013439038295626	19.77729978086514
9	24.431098651050856	14.511504159862499	21.800975763986877	39.25642142509977
10	17.117745887647555	26.70182690868484	33.176816439655504	23.0036107640121
11	33.163530442591885	22.356296805810857	23.163042728775622	21.317130022821644
12	21.085381871509274	26.854531786327527	30.635995634120462	21.424090708042737
13	31.472845271950906	20.60944045771101	26.214449446781174	21.703264823556914
14	20.779803939045863	22.88319590454936	27.26824764425818	29.068752512146595
15	23.288671080757	28.897548144921636	25.30276096473156	22.5110198095898
16	22.18004712324528	27.89521216391893	26.57064870982878	23.354092003007008
17	21.893473211898197	26.42080284421243	26.884971855549257	24.800752088340108
18	23.210973224511488	25.099939288038737	29.095829037807913	22.59325844964187
19	23.072563407000544	24.733649394309893	30.118514457351107	22.075272741338456
20	24.11156201281174	24.136284057980763	30.012226480842095	21.7399274483654
21	22.98275679393755	24.951775194202597	30.425101452882643	21.640366558977213
22	21.75909964665975	25.369863658761776	30.0473755110484	22.82366118353007
23	22.488147713379	25.65845569624511	31.16777187102156	20.685624719354333
24	22.587372248411146	25.64718782531773	28.793951003260954	22.97148892301017
25	22.529519299172062	25.76171648354975	29.581692905109392	22.1270713121688
26	22.138339183096175	26.526418112009363	29.64122762612868	21.694015078765783
27	21.332265968843497	26.586457364562715	29.28620560332722	22.795071063266573
28	21.74194557450165	26.139274248205968	29.538303193180077	22.580476984112302
29	22.02465141075426	26.166687128223327	28.926138265185138	22.88252319583728
30	21.855969701199605	26.259184576134647	30.55358881689037	21.331256905775373
31	22.444589824271667	25.686541284974545	30.59277409936917	21.27609479138462
32	22.112271720502985	25.691250245959125	29.425288129550243	22.771189903987647
33	21.6933423700537	25.82125120456904	30.060325153755983	22.425081271621277
34	21.269367704263793	26.206208765058165	30.53626656755425	21.98815696312379
35	20.83092980116412	28.09231581655906	29.40409780511965	21.672656577157166
36	22.721745813649598	25.808637916217492	29.417383802183277	22.052232467949633
37	21.757922406413606	26.402471531808192	29.372816850007823	22.466789211770386
38	21.68493351115267	25.140638165119718	30.6428908984193	22.531537425308308
39	21.0472056520986	24.973806404523295	31.44206884837314	22.53691909500497
40	20.296126375058652	25.75028043544435	30.937369137133352	23.016224052363647
41	21.43367680718991	24.69009150520256	30.906088182021524	22.970143505586005
42	20.205478876105552	26.617906496852562	30.390961485744462	22.78565314129742
43	20.57277783290252	26.429884411825544	29.59598796524114	23.401349790030793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27.0
1	58.5
2	90.0
3	309.0
4	528.0
5	528.0
6	786.5
7	1045.0
8	1159.5
9	1274.0
10	1880.5
11	2487.0
12	2487.0
13	4545.5
14	6604.0
15	10251.5
16	13899.0
17	12864.0
18	11829.0
19	11829.0
20	12732.0
21	13635.0
22	10760.0
23	7885.0
24	8608.0
25	9331.0
26	9331.0
27	10151.0
28	10971.0
29	11831.5
30	12692.0
31	14145.5
32	15599.0
33	15599.0
34	17595.0
35	19591.0
36	21724.0
37	23857.0
38	25705.5
39	27554.0
40	27554.0
41	28908.5
42	30263.0
43	32778.0
44	35293.0
45	37896.5
46	40500.0
47	40500.0
48	51660.0
49	62820.0
50	60177.5
51	57535.0
52	53110.5
53	48686.0
54	48686.0
55	43575.5
56	38465.0
57	32943.5
58	27422.0
59	24195.5
60	20969.0
61	20969.0
62	18659.5
63	16350.0
64	14100.5
65	11851.0
66	10229.5
67	8608.0
68	8608.0
69	7424.0
70	6240.0
71	5208.5
72	4177.0
73	3469.0
74	2761.0
75	2761.0
76	2218.5
77	1676.0
78	1312.5
79	949.0
80	740.0
81	531.0
82	531.0
83	409.0
84	287.0
85	211.0
86	135.0
87	112.5
88	90.0
89	90.0
90	76.5
91	63.0
92	45.0
93	27.0
94	18.0
95	9.0
96	9.0
97	6.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	594611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.85465638078664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51507309927165	50.32960242635484
2	8.049650736146793	9.475188561225316
3	2.296369881924026	4.054561809714785
4	1.108601522751867	2.6098544673911186
5	0.6330687161949243	1.8629520878539005
6	0.45274574392869943	1.5987717112072337
7	0.28972122465661804	1.1936010186370163
8	0.23929108925657033	1.1266715866543675
9	0.19041023688351957	1.008587615684736
>10	1.0831107739303594	11.960921601891254
>50	0.09158515055873352	3.636877357772649
>100	0.04350293933765042	4.758377801422712
>500	0.0040068496758359955	1.569899150740142
>1k	0.0022896283861919974	2.6290757451450792
>5k	5.724070965479993E-4	2.1850570583048414
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6604	1.110642083647965	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6368	1.070952269635106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4853	0.8161638449339148	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2540	0.42717003217229416	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1491	0.2507521724286971	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1420	0.23881159278923533	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1405	0.2362889351189265	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1382	0.23242086002445297	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1361	0.22888913928602062	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1156	0.19441281779180003	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	842	0.14160518389333532	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	817	0.1374007544428206	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	781	0.13134637603407942	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	718	0.12075121381878236	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	703	0.11822855614847354	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	680	0.114360481054	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	675	0.11351959516389706	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	669	0.11251053209577354	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	638	0.10729703957713531	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.10393349601672355	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	609	0.10241990141453824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.363543560411765E-4	0.0	0.0	0.0	0.0
8	3.363543560411765E-4	0.0	0.0	0.0	0.0
9	3.363543560411765E-4	0.0	0.0	3.363543560411765E-4	0.0
10	5.045315340617648E-4	0.0	0.0	3.363543560411765E-4	0.0
11	5.045315340617648E-4	0.0	0.0	5.045315340617648E-4	0.0
12	6.72708712082353E-4	0.0	0.0	0.0010090630681235295	0.0
13	6.72708712082353E-4	0.0	0.0	0.0010090630681235295	0.0
14	6.72708712082353E-4	0.0	0.0	0.0015135946021852943	0.0
15	6.72708712082353E-4	0.0	0.0	0.002186303314267647	0.0
16	6.72708712082353E-4	0.0	0.0	0.003363543560411765	0.0
17	6.72708712082353E-4	0.0	0.0	0.0048771381625970596	0.0
18	6.72708712082353E-4	0.0	0.0	0.005213492518638236	0.0
19	6.72708712082353E-4	0.0	0.0	0.0065589099428029415	0.0
20	6.72708712082353E-4	0.0	0.0	0.010426985037276472	0.0
21	6.72708712082353E-4	0.0	0.0	0.0171540721581	0.0
22	6.72708712082353E-4	0.0	0.0	0.02875829744152059	0.0
23	6.72708712082353E-4	0.0	0.0	0.03380361278213824	0.0
24	6.72708712082353E-4	0.0	0.0	0.04137158579306471	0.0
25	6.72708712082353E-4	0.0	0.0	0.047762318557847065	0.0
26	6.72708712082353E-4	0.0	0.0	0.06054378408741177	0.0
27	6.72708712082353E-4	0.0	0.0	0.1173876702583706	0.0
28	6.72708712082353E-4	0.0	0.0	0.22872096210800003	0.0
29	6.72708712082353E-4	0.0	0.0	0.3883211040495383	0.0
30	6.72708712082353E-4	0.0	0.0	0.6088013844345295	0.0
31	6.72708712082353E-4	0.0	0.0	0.9226199986209471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	35	8.8643417E-4	26.42857	1
GGTATCA	2065	0.0	21.769976	1
GACCGTC	60	3.7223934E-5	21.583334	7
CGACCGC	45	0.0038236687	20.555555	10
CCGACCG	45	0.0038236687	20.555555	9
ATTAGAG	100	1.2865712E-8	20.349998	3
TTAGACC	55	5.139081E-4	20.181818	4
TGTTACG	55	5.139081E-4	20.181818	16
GTATTAG	110	3.8422513E-8	18.5	1
TGCACCG	60	9.229669E-4	18.5	5
CTATCCT	50	0.007030987	18.499998	4
TAGGTCG	50	0.007030987	18.499998	21
CATTGCG	50	0.007030987	18.499998	29
AGTTCCG	50	0.007030987	18.499998	5
ACCGTCG	50	0.007030987	18.499998	8
TAACGGC	75	2.0651534E-4	17.266666	36
TTAACGG	75	2.0651534E-4	17.266666	35
GCCTAGT	75	2.0651534E-4	17.266666	1
GGGTCGA	65	0.0015787218	17.076923	10
TGTGCGC	65	0.0015787218	17.076923	10
>>END_MODULE