FastQCFastQC Report
Wed 25 May 2016
SRR2088616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039262
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT226081.108636359624217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225541.105988342841675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT167800.8228466965009892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90020.441434205119303No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG56280.2759821935582578No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53660.26313440842814706No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA52180.2558768809500692No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA47770.2342514105593102No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG47690.2338591117767114No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG39190.19217736612558856No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA29300.14367942912681156No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29250.14343424238768732No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.1313710548227741No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26020.12759517904026063No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG25800.12651635738811393No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC24190.11862134438831304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23350.11450220717102559No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.11406087104060193No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA22820.11190322773630852No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22550.11057921934503757No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC21870.10724467969294774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73600.023.0747281
TATACCG1054.801768E-717.619055
GTATTAG3900.017.0769231
TCTATAC1452.9831426E-916.5862063
CTAATAC3700.016.53
GTATTGG14000.016.1214281
GTTCTAA1902.7284841E-1115.5789481
TCTAATA4400.015.5568182
TTAACGG2400.015.41666735
GTATCAA110850.015.32070352
GTCGGGT2300.015.28260924
ATTGGAC14450.015.1072673
TTGGACC18100.014.8204424
CGGTATA1001.0940232E-414.826
GAGTAGT1752.240995E-914.7999991
ACCGTTA3500.014.7999998
TGGACCC18000.014.4916675
TATTGGA15450.014.4886722
TAATACT4100.014.4390254
CTCTAAT3850.014.4155841
Produced by FastQC (version 0.11.5)