FastQCFastQC Report
Wed 25 May 2016
SRR2088615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424647
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT311580.9098163985952421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289740.8460434024295058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224260.6548412142915752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122250.35697109804309757No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG68760.20077981759871896No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA65870.19234099164089027No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA65620.19161098939540339No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG61520.17963895256941811No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA58340.17035332400682465No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG50070.14620484972611777No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36830.10754393080513115No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35690.10421512056571086No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34440.10056510933827634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98850.022.0091061
GTATTGG16950.016.8082581
ACCGTTA4800.016.18758
GGACCGT6950.015.1726626
GTATCAA147850.014.7524532
TAATACT9100.014.6373634
ATTGGAC21250.014.5388233
TGTTACG3550.014.07042216
GTATTAG9250.014.01
TTAACGG5450.013.91743135
TTGGACC25150.013.8290264
TGGACCC23650.013.68921855
TATACTG4600.013.6739125
GACCGTT5600.013.5446427
ATTAGAG8400.013.4345253
GGACCCT25700.013.3891056
CTAATAC8850.013.1694913
TACCCTC8750.012.8971435
CGTTATT4050.012.79012310
CCGTTTA5950.012.74789927