##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3424647
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71049103747043	31.0	31.0	34.0	30.0	34.0
2	31.892589805606242	31.0	31.0	34.0	30.0	34.0
3	31.971985141826295	31.0	31.0	34.0	30.0	34.0
4	35.66036762329081	37.0	35.0	37.0	33.0	37.0
5	35.558937023290284	37.0	35.0	37.0	33.0	37.0
6	35.587186358185235	37.0	35.0	37.0	33.0	37.0
7	35.46286376376894	37.0	35.0	37.0	33.0	37.0
8	35.475999424174226	37.0	35.0	37.0	33.0	37.0
9	37.091818806434645	39.0	37.0	39.0	33.0	39.0
10	36.952112144696954	39.0	37.0	39.0	33.0	39.0
11	36.9982690186755	39.0	37.0	39.0	33.0	39.0
12	36.900110872741045	39.0	37.0	39.0	33.0	39.0
13	36.945331299839076	39.0	37.0	39.0	33.0	39.0
14	38.002471203601424	40.0	37.0	41.0	33.0	41.0
15	37.960291381856294	40.0	37.0	41.0	33.0	41.0
16	37.86334533165024	40.0	37.0	41.0	32.0	41.0
17	37.873381694522095	40.0	37.0	41.0	33.0	41.0
18	37.85493891779211	40.0	37.0	41.0	33.0	41.0
19	37.874644014404986	40.0	37.0	41.0	32.0	41.0
20	37.75456156503137	40.0	37.0	41.0	32.0	41.0
21	37.65485815034367	40.0	37.0	41.0	32.0	41.0
22	37.50817412714361	39.0	36.0	41.0	32.0	41.0
23	37.3917960011645	39.0	36.0	41.0	32.0	41.0
24	37.2589653765775	39.0	36.0	41.0	31.0	41.0
25	37.066354576106676	39.0	36.0	41.0	31.0	41.0
26	37.22914011283499	39.0	36.0	41.0	31.0	41.0
27	37.16214342675318	39.0	36.0	41.0	31.0	41.0
28	37.048154160122195	39.0	36.0	41.0	31.0	41.0
29	36.913380561558604	39.0	36.0	41.0	30.0	41.0
30	36.804687023217284	39.0	35.0	41.0	30.0	41.0
31	36.64665993312595	39.0	35.0	40.0	30.0	41.0
32	36.53976950033098	39.0	35.0	40.0	30.0	41.0
33	36.35674946936137	39.0	35.0	40.0	30.0	41.0
34	36.23132982757055	38.0	35.0	40.0	30.0	41.0
35	36.062064498910395	38.0	35.0	40.0	29.0	41.0
36	35.92853278016683	38.0	35.0	40.0	28.0	41.0
37	35.779456393607866	38.0	35.0	40.0	27.0	41.0
38	35.718514930151926	38.0	35.0	40.0	27.0	41.0
39	35.60021952627526	38.0	35.0	40.0	26.0	41.0
40	35.43631241409699	38.0	34.0	40.0	26.0	41.0
41	35.32520432032849	38.0	34.0	40.0	25.0	41.0
42	35.13008902815385	38.0	34.0	40.0	24.0	41.0
43	34.78730450174865	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	3.0
14	5.0
15	21.0
16	33.0
17	98.0
18	208.0
19	511.0
20	1032.0
21	2076.0
22	4003.0
23	7167.0
24	11733.0
25	17824.0
26	26437.0
27	37419.0
28	51332.0
29	67748.0
30	85980.0
31	107580.0
32	132733.0
33	164553.0
34	204263.0
35	254200.0
36	330801.0
37	464854.0
38	659622.0
39	792404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71037190110397	20.960496074485928	13.475491050610472	23.853640973799635
2	18.910883369877247	22.544133745755403	35.255692046508734	23.289290837858616
3	19.66666929467475	24.59450565269939	31.912515362897253	23.826309689728607
4	13.958927737661723	16.12574960280578	36.24653869435303	33.66878396517948
5	13.188191366876644	37.918623437685696	35.076637095735705	13.816548099701956
6	32.81395133571431	36.91752755831477	14.041593191940658	16.226927914030263
7	26.51444075841977	32.242651578396256	21.979725209634747	19.263182453549227
8	26.439571728122637	34.847387190563	19.29375494759022	19.41928613372415
9	24.6729370939545	14.464322892257217	21.532029432522535	39.33071058126575
10	16.553092917313812	27.052773614331638	33.93278197723736	22.46135149111719
11	33.517936301172064	22.23814600453711	23.249461915344853	20.994455778945976
12	20.87540701275197	26.73992385200577	30.949145999573098	21.435523135669165
13	30.83173243840898	20.833300483232286	26.150782839808016	22.184184238550717
14	20.741845801917684	22.45288346506954	27.32269924462288	29.482571488389897
15	23.268617174266428	29.02141446987091	25.12019486971942	22.589773486143244
16	22.512772849289284	27.86208330376824	26.30671131944402	23.31843252749845
17	22.0514698303212	26.533216416173694	27.006987873494698	24.408325880010405
18	22.89205281595446	25.711204687665617	28.937697812358472	22.45904468402145
19	23.10287746445108	24.96961000652038	29.73106425275364	22.196448276274896
20	23.842982941015524	24.750346532065933	29.52552482051435	21.881145706404194
21	22.89053441128385	25.486772797313122	29.822139332900587	21.80055345850244
22	21.964745563557354	25.82657424254237	29.464467432701824	22.744212761198455
23	22.549418962012727	25.995642762597136	30.298392797856245	21.15654547753389
24	22.748709575030652	26.125086760766873	28.151368593609792	22.97483507059268
25	22.771485645089847	25.94722901367645	29.07160942427059	22.20967591696312
26	22.44222543228543	26.596230210004123	29.109131539688615	21.852412818021826
27	21.738941268983343	26.615327068746065	28.77654251664478	22.86918914562581
28	21.952393925563715	26.286417257019483	29.168349321842513	22.59283949557429
29	22.35529676489285	26.36759350671763	28.588435538027714	22.68867419036181
30	22.219808348130478	26.339649020760387	29.877006301671383	21.56353632943775
31	22.52541648818112	26.040815301547866	29.810722097781174	21.623046112489842
32	22.16105776741369	25.807418983620796	29.05043935915147	22.981083889814045
33	21.947517510563863	25.93776818457493	29.61610349913436	22.498610805726837
34	21.61592129057389	26.228893080075117	29.986477438404602	22.168708190946397
35	21.35227367959384	27.662354689403024	29.038029320978193	21.947342310024943
36	22.857801110596217	25.883368417241254	29.059491386995507	22.199339085167026
37	21.951517922869133	26.170726501154718	29.13403921630463	22.743716359671524
38	21.920040226043735	25.084220359061828	30.266272699054824	22.72946671583962
39	21.319598779085844	25.172842631663933	30.807671564397733	22.69988702485249
40	20.640579890423744	25.718095908863013	30.5529007807228	23.088423419990438
41	21.63931056251929	24.677229507157964	30.628529013355244	23.0549309169675
42	20.392846328395304	26.38055834659748	30.247818242288915	22.978777082718306
43	20.850674536674873	26.00484079089027	29.6064090693143	23.538075603120557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	378.5
2	572.0
3	1556.5
4	2541.0
5	2541.0
6	3932.0
7	5323.0
8	5755.0
9	6187.0
10	9079.5
11	11972.0
12	11972.0
13	22025.5
14	32079.0
15	48603.0
16	65127.0
17	60661.5
18	56196.0
19	56196.0
20	61274.0
21	66352.0
22	54715.5
23	43079.0
24	49019.5
25	54960.0
26	54960.0
27	61270.0
28	67580.0
29	74856.0
30	82132.0
31	92126.5
32	102121.0
33	102121.0
34	115491.5
35	128862.0
36	141801.5
37	154741.0
38	166225.0
39	177709.0
40	177709.0
41	185016.5
42	192324.0
43	205245.0
44	218166.0
45	225985.0
46	233804.0
47	233804.0
48	282494.0
49	331184.0
50	317695.5
51	304207.0
52	284970.0
53	265733.0
54	265733.0
55	241671.5
56	217610.0
57	187927.5
58	158245.0
59	141926.5
60	125608.0
61	125608.0
62	111311.0
63	97014.0
64	84721.0
65	72428.0
66	62014.0
67	51600.0
68	51600.0
69	44309.0
70	37018.0
71	31316.5
72	25615.0
73	20813.5
74	16012.0
75	16012.0
76	12631.5
77	9251.0
78	7256.5
79	5262.0
80	4022.0
81	2782.0
82	2782.0
83	2106.0
84	1430.0
85	1091.0
86	752.0
87	583.5
88	415.0
89	415.0
90	356.5
91	298.0
92	199.0
93	100.0
94	70.5
95	41.0
96	41.0
97	32.0
98	23.0
99	15.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3424647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.30959348116737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26202429717026	37.02544554512368
2	12.017284497993938	11.370656886952553
3	3.990261528295838	5.663329523616531
4	1.7433604964784495	3.299107055180862
5	0.9466600314382451	2.2393050626106246
6	0.55897220255157	1.5866848601992514
7	0.40187139041304654	1.330866047850691
8	0.27201864061136927	1.0295273045298916
9	0.215321257205274	0.9168085031611855
>10	1.3448644966699974	12.135176786128708
>50	0.13978616276298872	4.607238106222409
>100	0.09366551932108756	8.573765379601586
>500	0.009087526338057253	2.9574895218406847
>1k	0.004203753679542457	3.4098489529628546
>5k	3.709194423125697E-4	1.0826560699618226
>10k+	2.472796282083798E-4	2.772094394056714
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31158	0.9098163985952421	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28974	0.8460434024295058	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22426	0.6548412142915752	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12225	0.35697109804309757	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6876	0.20077981759871896	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6587	0.19234099164089027	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6562	0.19161098939540339	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6152	0.17963895256941811	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5834	0.17035332400682465	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5007	0.14620484972611777	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3683	0.10754393080513115	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3569	0.10421512056571086	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3444	0.10056510933827634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.9200089819476284E-5	0.0
2	0.0	0.0	0.0	2.9200089819476284E-5	0.0
3	0.0	0.0	0.0	2.9200089819476284E-5	0.0
4	0.0	0.0	0.0	2.9200089819476284E-5	0.0
5	0.0	5.840017963895257E-5	0.0	2.9200089819476284E-5	0.0
6	2.9200089819476284E-5	5.840017963895257E-5	0.0	2.9200089819476284E-5	0.0
7	5.840017963895257E-5	8.760026945842885E-5	0.0	2.9200089819476284E-5	0.0
8	1.4600044909738143E-4	8.760026945842885E-5	0.0	5.840017963895257E-5	0.0
9	1.4600044909738143E-4	8.760026945842885E-5	0.0	2.04400628736334E-4	0.0
10	1.752005389168577E-4	8.760026945842885E-5	0.0	2.3360071855581027E-4	0.0
11	2.04400628736334E-4	1.1680035927790514E-4	0.0	4.3800134729214424E-4	0.0
12	2.3360071855581027E-4	1.1680035927790514E-4	0.0	9.052027844037648E-4	0.0
13	2.3360071855581027E-4	1.1680035927790514E-4	0.0	0.00102200314368167	0.0
14	2.6280080837528657E-4	1.1680035927790514E-4	0.0	0.0011096034131400988	0.0
15	2.6280080837528657E-4	1.4600044909738143E-4	0.0	0.0014892045807932905	0.0
16	2.6280080837528657E-4	1.4600044909738143E-4	0.0	0.00204400628736334	0.0
17	2.6280080837528657E-4	1.4600044909738143E-4	0.0	0.0026864082633918183	0.0
18	2.9200089819476287E-4	1.4600044909738143E-4	0.0	0.003182809790322915	0.0
19	2.9200089819476287E-4	1.4600044909738143E-4	0.0	0.003825211766351393	0.0
20	2.9200089819476287E-4	1.4600044909738143E-4	0.0	0.005956818323173162	0.0
21	2.9200089819476287E-4	1.4600044909738143E-4	0.0	0.010745633053567273	0.0
22	3.212009880142391E-4	1.752005389168577E-4	0.0	0.01877565775392325	0.0
23	3.212009880142391E-4	1.752005389168577E-4	0.0	0.023798073202873173	0.0
24	3.212009880142391E-4	1.752005389168577E-4	0.0	0.03247049987925763	0.0
25	3.212009880142391E-4	1.752005389168577E-4	0.0	0.038952919819181364	0.0
26	3.212009880142391E-4	1.752005389168577E-4	0.0	0.049464952154192825	0.0
27	3.212009880142391E-4	1.752005389168577E-4	0.0	0.09965990655387257	0.0
28	3.504010778337154E-4	1.752005389168577E-4	0.0	0.21406585846658063	0.0
29	3.504010778337154E-4	1.752005389168577E-4	0.0	0.3581975018155156	0.0
30	3.504010778337154E-4	2.04400628736334E-4	0.0	0.5562909111508427	0.0
31	3.504010778337154E-4	2.04400628736334E-4	0.0	0.8590374423991728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9885	0.0	22.009106	1
GTATTGG	1695	0.0	16.808258	1
ACCGTTA	480	0.0	16.1875	8
GGACCGT	695	0.0	15.172662	6
GTATCAA	14785	0.0	14.752453	2
TAATACT	910	0.0	14.637363	4
ATTGGAC	2125	0.0	14.538823	3
TGTTACG	355	0.0	14.070422	16
GTATTAG	925	0.0	14.0	1
TTAACGG	545	0.0	13.917431	35
TTGGACC	2515	0.0	13.829026	4
TGGACCC	2365	0.0	13.6892185	5
TATACTG	460	0.0	13.673912	5
GACCGTT	560	0.0	13.544642	7
ATTAGAG	840	0.0	13.434525	3
GGACCCT	2570	0.0	13.389105	6
CTAATAC	885	0.0	13.169491	3
TACCCTC	875	0.0	12.897143	5
CGTTATT	405	0.0	12.790123	10
CCGTTTA	595	0.0	12.747899	27
>>END_MODULE